Recombinant proteins
Establishment and evaluation of flexible insect cell lines for rapid production of recombinant proteins
... A flexible Sf9 insect cell line was recently developed leveraging the recombinase mediated cassette exchange (RMCE) technology, which competes with the popular baculovirus expression vector system (BEVS) in terms of ...
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A double built-in containment strategy for production of recombinant proteins in transgenic rice.
... valuable proteins at a large scale ...of recombinant pharmaceutical proteins and industrial enzymes ...of recombinant proteins have been successfully expressed in rice seeds, including ...
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Analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in Asian seabass.
... The recombinant proteins of the c-type and g-type lysozymes showed lytic activities against the bacterial pathogens Vibrio harveyi and Photobacterium damselae in a dosage-dependent ...
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Potencial antigênico de proteínas recombinantes de Leptospira Interrogans / Antigenic potential of recombinant proteins from Leptospira Interrogans
... of recombinant proteins from Leptospira interrogans serovar Copenhageni, in order to select candidates for the development of a recombinant vaccine against ...The recombinant proteins ...
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Production of Recombinant Proteins in Milk of Transgenic and Non-transgenic Goats
... produce recombinant proteins from years to weeks (Bösze et ...of recombinant proteins expressed in the milk of different species at the same time (Sanchez et ...various recombinant ...
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Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins.
... remaining recombinant proteins (rH2B, rH3, rH4 and rKMP11) presented lower values for AUC and likelihood ...other recombinant proteins ...employing recombinant proteins, serum ...
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Immunogenicity of Recombinant Proteins Consisting of Plasmodium vivax Circumsporozoite Protein Allelic Variant-Derived Epitopes Fused with Salmonella enterica Serovar Typhimurium Flagellin
... 3 recombinant fusion proteins, each of which expressed 1 of the 3 allelic ...the recombinant fusion proteins was assessed by im- munizing mice with the recombinant proteins ...
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Recombinant proteins in differentiation of stem cells
... of recombinant proteins in cardiac differentiation, Fonoudi and collaborators (2013) developed a recombinant protein which was added to hESCs cultures, and efficiently penetrated into the cells, ...
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Production of recombinant proteins in Escherichia coli
... of recombinant genes is often desirable or necessary since leaky expression can be detrimental or even lethal to cell ...of recombinant proteins due to two main reasons: First, they do not allow the ...
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Optimization of a gellan gum support by experimental design for recombinant proteins partition
... (NaCl) and bromophenol blue were acquired from Sigma-Aldrich (St. Louis, MO). Acrylamide 30% / Bis-acrylamide solution was obtained from BioRad (Hercules, CA). Tris(hydroxymethyl)aminomethane (Tris) was bought from ...
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Targeted amino-terminal acetylation of recombinant proteins in E. coli.
... The lack of Nat complexes or their equivalents within prokaryotes has prevented the use of E. coli expression systems for producing N-terminally acetylated proteins. Although gram- negative bacteria, such as E. ...
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Improvement of STEAP 1 Biosynthesis from Pichia pastoris X33 cells under an optimized feeding strategy
... of proteins, stability, immunogenicity, and biological functions, that take part in many physiological processes ...therapeutic proteins, for instance enzymes and ion channels, is mandatory the study of ...
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Rev. Soc. Bras. Med. Trop. vol.41 suppl.2 en v41s2a18
... leprae proteins with potential diagnostic ...reactive recombinant Mycobacterium leprae proteins were identiied and may be used in conjunction with PGL-I to improve MB patient ...leprae ...
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Structural characterization and comparative analysis of human and piscine cartilage acidic protein (CRTAC1/CRTAC2)
... three recombinant proteins [human (h) CRTAC1 and sea bass (Dicentrarchus labrax, dl) CRTAC1 and CRTAC2] were over-expressed in ...refolded recombinant proteins were obtained with a ...
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Mutant and chimeric recobinant plasminogen activatorsproduction in eukaryotic cellsand preliminary characterization
... In the present study, we found that the recombinant proteins, produced by transfected eukaryotic cells, are efficiently secreted in the culture medium and, in most instanc[r] ...
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Characterization of protein phosphatase 5 from three lepidopteran insects: Helicoverpa armigera, Mythimna separata and Plutella xylostella.
... predicted proteins of 490 ...coli-produced recombinant PP5 proteins could be enhanced by almost 2-fold by a known PP5 activator: arachidonic ...three recombinant proteins against ...
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Novel recombinant multiepitope proteins for the diagnosis of asymptomatic leishmania infantum-infected dogs.
... insert. Recombinant plasmids were used to transform ...of recombinant proteins were affinity purified using an A ¨ KTA Prime chromatog- raphy system (GE Healthcare Life Science) with a 5 mL HIS-Trap ...
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An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.
... Accurate FRET efficiencies can be obtained by measuring changes to the donor fluorescence lifetime in the presence of the acceptor [34], and FRET-induced changes to CFP lifetimes can be observed by fluorescence lifetime ...
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Recombinant Technology and Probiotics
... of recombinant probiotic carrier organisms is crucial, especially when the strain has to be used under diseased ...of recombinant Escherichia coli Nissle 1917 (rEcN) to serve as a safe carrier for targeted ...
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Baculovirus superinfection: a probable restriction factor on the surface display of proteins for library screening.
... of recombinant proteins, baculoviruses have been developed as a platform for the display of complex eukaryotic proteins on the surface of virus particles or infected insect ...two recombinant ...
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