16S rDNA
RFLP, and 16S rDNA sequencing
... Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. ...
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Identidade molecular dos fitoplasmas associados aos enfezamentos do tomateiro e da berinjela com base na análise do gene 16S rDNA.
... A extração de DNA total foi feita a partir de material foliar (16) Para detecção de fitoplasma foi feita a amplificação da região do DNA genômico correspondente ao 16S rDNA. Iniciadores (“primers”) ...
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Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy
... by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was ...later 16S rDNA amplicons were utilized to construct the WGA library for further clone ...
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Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis
... Analyzing clones distribution in the different phyla, the majority of the NFA sequences, 44.78%, fell within the Acidobacteria phylum (Table 2). It was expected to find a larger percentage of this phylum in EAA, given ...
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IDENTIFICAÇÃO DE BACTÉRIAS CONTAMINANTES DE FERMENTO DE CACHAÇA POR SEQÜENCIAMENTO DO GENE 16S RDNA
... ABSTRACT: Cachaça is a typical Brazilian liquor produced from the distillation of fermented sugarcane juice mainly by Saccharomyces cerevisiae. Most of the domestic production is artisanal, and producers usually are not ...
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A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks ( Amblyomma cajennense ) from Rio de Janeiro, Brazil
... sp. 16S rDNA specific primer set, all samples proved to be positive for Eubacteria, although only one presented positive amplifi- cation using the Rickettsia primer ...
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Xiao Lei Liu, Su Lin Liu, Min Liu, Bi He Kong, Lei Liu, Yan Hong Li
... About 2 g of surface-disinfected G. montana was frozen with liquid nitrogen and ground to a fine powder in a sterilized and precooled mortar. Next, the cetyltrimethyl- ammonium bromide (CTAB) procedure was used to ...
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16S-23S RDNA: POLYMORPHISMS AND THEIR USE FOR DETECTION AND IDENTIFICATION OF XYLELLA FASTIDIOSA STRAINS
... The rDNA genetic locus is extremely important in all organisms and there is sufficient conservation for it to be used in a universal characterization of relationship of many bacterial species ...subunit, ...
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Molecular analysis of Muticaria syracusana and M. neuteboomi from Southeastern Sicily, Italy (Gastropoda, Pulmonata, Clausiliidae)
... ribosomal 16S rDNA subunit and the cytochrome oxydase I (COI) mitochondrial genes by Neighbour Joining, Maximum Likelihood, Maximum Parsimony and Bayesian Inference ...for 16S rDNA and 12% for ...
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Phylogeography of the Land Snail Eobania vermiculata (O.F. Müller, 1774) (Gastropoda: Pulmonata) along the Croatian Coast and Islands
... 0.0039±0.0005 (table 2). Partial COI gene sequences (523 bp) were obtained for 74 individuals and grouped into 13 haplotypes. The most frequent COI haplotypes were H1, H2 and H7 and only three haplotypes were unique ...
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Characterization of the core microbiota of the drainage and surrounding soil of a Brazilian copper mine
... a 16S rDNA pyrosequencing analysis was performed to characterize the core microbiota of a neu- tral mine drainage and the surrounding high heavy metal content soil, in order to obtain new insights into ...
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Correction: Genomic and Genetic Diversity within the Pseudomonas fluorescens Complex.
... Fig 2. Phylogeny of the P. fluorescens complex inferred by MLSA. Phylogenetic tree of 127 sequenced and type strains belonging to the P. fluorescens complex based on the concatenated partial sequences of the 16S ...
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The canine oral microbiome.
... length 16S rDNA reference sequences, which are required for recognition and placement of previously unrecognized rare taxa members such as those in candidate divisions GN02 and ...
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The chitinase C gene PsChiC from Pseudomonas sp. and its synergistic
... the 16S rDNA sequence analysis, strain TXG6-1 belongs to the genus Pseudomonas, and comparison of the amino acid sequence of PsChiC from strain TXG6-1 with the chiC se- quences of other bacterial chitinase ...
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Endophytic bacterial diversity in banana ‘Prata Anã’ ( Musa spp.) roots
... Various molecular techniques have been used to char- acterize endophytic bacteria, including repetitive extra- genic palindromic sequence PCR (rep-PCR), which uses the conserved sequences of ERIC, REP and BOX, 16S ...
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Feeding bovine milks with low or high IgA levels is associated with altered re-establishment of murine intestinal microbiota after antibiotic treatment
... for 16S rDNA sequencing data of bacterial communities (PC1 versus PC2, PC1 versus PC3 and PC2 versus PC3) in individual mice that were exposed to antibiotics and then fed water/milk for a period of 14 days ...
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Construction of a phylogenetic tree of photosynthetic prokaryotes based on average similarities of whole genome sequences.
... of 16S rDNA and conserved orthologous genes, our tree differed slightly from trees based on single gene comparisons especially for the closely related ...Although rDNA is a good marker for ...
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16SrXV phytoplasma in Costa Rica
... The 16S rDNA sequence (about 1460 nt), obtained from the P1A/16S-SR semi-nested-PCR products of two phytoplasma strains (GenBank accession numbers: HQ258882 and HQ258883), shared ...
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MATERIALS AND METHODS Soil and rhizosphere samples
... amostras de solo com etanol e posterior agitação com partículas foi uma estratégia bem sucedida para lise das células sem degradação significativa dos ácidos nucléicos, resultando em bom rendimento de DNA e RNA íntegros. ...
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BACTERIAL DIVERSITY CHARACTERIZATION IN PETROLEUM SAMPLES FROM BRAZILIAN RESERVOIRS Valéria Maia de Oliveira1 ; Lara Durães Sette1 ; Karen Christina Marques Simioni1 ; Eugênio Vaz dos Santos Neto2
... protocols shall be tested and adapted for the analysis of such water and oil samples, where the abundance of microbial groups is very low. Yoshida et al. (44) have recently used 2,2,4- trimethylpentane (isooctane) to ...
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