SH-SY5Y
Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
... animals) SH- SY5Y cells into the abdominal cavity on day ...the SH-SY5Y, control siRNA-transfected, and survivin siRNA-transfected groups, ...
6
Gadd153 and NF-κB crosstalk regulates 27-hydroxycholesterol-induced increase in BACE1 and β-amyloid production in human neuroblastoma SH-SY5Y cells.
... same study also demonstrated that BACE1 elevation in the neurons that surround the amyloid plaques is an early event preceding the subsequent neuronal loss that eventually occurs, thereby dispelling the speculation that ...
13
Identification of novel microRNAs in post-transcriptional control of Nrf2 expression and redox homeostasis in neuronal, SH-SY5Y cells.
... Nuclear factor-erythroid 2-related factor 2 (Nrf2/NFE2L2), a redox-sensitive transcription factor plays a critical role in adaptation to cellular stress and affords cellular defense by initiating transcription of ...
16
Hydrogen sulfide inhibits A2A adenosine receptor agonist induced β-amyloid production in SH-SY5Y neuroblastoma cells via a cAMP dependent pathway.
... samples were diluted by standard diluent buffer and AEBSF was added to diluted samples and standards which prevent proteolysis of Ab peptides. The standards, controls and samples were pipetted into the antibody ...
12
The TrkAIII oncoprotein inhibits mitochondrial free radical ROS-induced death of SH-SY5Y neuroblastoma cells by augmenting SOD2 expression and activity at the mitochondria, within the context of a tumour stem cell-like phenotype.
... in SH-SY5Y ...TrkAIII SH-SY5Y cell ...TrkAIII SH-SY5Y ...TrkAIII SH-SY5Y cell ...TrkAIII SH-SY5Y ...TrkAIII SH-SY5Y cell ...TrkAIII ...
20
Methyllycaconitine alleviates amyloid-β peptides-induced cytotoxicity in SH-SY5Y cells.
... The role of autophagy in Ab-mediated growth inhibition was further studied by siRNA knockdown of the expression of beclin 1, a component of the class III phosphatidy-linositol 3-kinase complex essential for autophagosome ...
9
Ropinirole alters gene expression pro files in SH-SY5Y cells: a whole genome microarray study
... Intriguingly, we observed the distinct promotion of PIK3C2B transcript and protein expression levels in SH- SY5Y cells following ROP treatment. This indicated that ROP might exert neuroprotective effects ...
6
Microglia are mediators of Borrelia burgdorferi-induced apoptosis in SH-SY5Y neuronal cells.
... Inflammation has long been implicated as a contributor to pathogenesis in many CNS illnesses, including Lyme neuroborreliosis. Borrelia burgdorferi is the spirochete that causes Lyme disease and it is known to potently ...
15
Synthesis and biological evaluation of mono and bis-naphthalimide derivatives against SH-SY5Y, human brain cancer cells
... Synthesis and biological evaluation of mono and bis-naphthalimide derivatives against SH-SY5Y, human brain cancer cells.. Master Thesis.[r] ...
169
Human ClC-6 is a late endosomal glycoprotein that associates with detergent-resistant lipid domains.
... Background. The mammalian CLC protein family comprises nine members (ClC-1 to -7 and ClC-Ka, -Kb) that function either as plasma membrane chloride channels or as intracellular chloride/proton antiporters, and that ...
13
Cytotoxicity of superoxide dismutase 1 in cultured cells is linked to Zn2+ chelation.
... The active SOD1 enzyme exists within the cell as a dimeric metallated holo protein, with each subunit containing one redox- active Cu 1+/2+ ion and one Zn 2+ ion. However, a small fraction of the SOD1 molecules are ...
12
Conseqüências da expressão da enzima Cu,Zn-superóxido dismutase (SOD1) e sua mutante...
... Comparar ao longo do tempo de cultura algumas das características dos neuroblastomas humanos parentais (SH-SY5Y) com aquelas dos transfectados com uma única cópia do gene SOD1 selvagem (SH-SY5YG WT ) ...
82
Novel 5' untranslated region directed blockers of iron-regulatory protein-1 dependent amyloid precursor protein translation: implications for down syndrome and Alzheimer's disease.
... in SH-SY5Y cells with a high degree of selectivity in the absence of changes to the levels of b-actin and a-synuclein ( SNCA ...of SH-SY5Y cells for 48 h to measure the capacity of JTR-009 and ...
14
Comparative in vitro study of the inhibition of human and hen esterases by methamidophos enantiomers
... in SH-SY5Y human neu- roblastoma cells the potential of the methamidophos enantiomers to induce delayed neurotoxicity using the ratio between NTE inhi- bition and AChE inhibition as a possible ...
6
Orientador: Doutor José Manuel Fuentes Rodríguez Co-orientador: Doutora Mireia Niso Santano Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Universidad de Extremadura, Espanha
... Rab7 has been localized to late endosomes and shown to be important in the late endocytic pathway. ER is anocther organelle that has an important role in endocytic pathway. Calnexin is a 67 kDa integral protein of the ...
94
LMNA knock-down affects differentiation and progression of human neuroblastoma cells.
... experiments, SH-SY5Y and SMS-KCNR cells were seeded at a density of 5610 3 cells/cm 2 . The following day cells were induced to differentiate by 10 mM all-trans retinoic acid (ATRA, named RA throughout the ...
14
RACK1 is a ribosome scaffold protein for β-actin mRNA/ZBP1 complex.
... To further investigate how Src may regulate the interaction of RACK1 with ZBP1, we co-expressed, in SH-SY5Y cells, Flag- ZBP1 with GFP-RACK1 wt , which is reported to inhibit the Src kin[r] ...
11
FBXO7 Y52C polymorphism as a potential protective factor in Parkinson's disease.
... Mutations in the F-box only protein 7 gene (FBXO7), the substrate-specifying subunit of SCF E3 ubiquitin ligase complex, cause Parkinson’s disease (PD)-15 (PARK15). To identify new variants, we sequenced FBXO7 cDNA in 80 ...
11
Nucleosome Repositioning: A Novel Mechanism for Nicotine- and Cocaine-Induced Epigenetic Changes.
... the SH-SY5Y cells and the microarray discussed above have permitted the very first description of the effects of drugs of abuse on chromatin structure at the level of DNA-nucleosome ...
19
Atividade citoprotetora da berberina, um alcalóide isoquinolínico, sobre a toxicidade celular induzida pela 6 (6OHDA) em células SHSY5Y
... células SH-SY5Y são úteis como um modelo para neurônios dopaminérgicos, pois possuem capacidade de sintetizar a DA e NA, por expressar TH e dopamina- β-hydroxilase (OYARCE; FLEMING, ...linhagem ...
95