Whole genome
The "most wanted" taxa from the human microbiome for whole genome sequencing.
... high-quality whole-genome sequences are available, (ii) the ‘‘GOLD-Human’’ database ...represents whole-genome sequenced strains isolated from humans that are being completed as part of the ...
11
Whole genome characterization, phylogenetic and genome signature analysis of human pandemic H1N1 virus in Thailand, 2009-2012.
... 25 m l. PCR reactions were performed in a thermal cycler (Eppendorf, Germany) under the following conditions: Initial denaturation at 94 uC for 3 minutes, followed by 40 amplification cycles comprising denaturation for ...
10
Prediction of expected years of life using whole-genome markers.
... Genetic factors are believed to account for 25% of the interindividual differences in Years of Life (YL) among humans. However, the genetic loci that have thus far been found to be associated with YL explain a very small ...
7
Evaluation of whole genome sequencing for outbreak detection of Salmonella enterica.
... of whole genome sequencing (WGS) has decreased dramatically and the technology becomes increasingly available for routine use around the world ...bacterial genome in the near future ...
11
Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences
... [11-13]. Whole genome sequencing has the potential to overcome such problems and extend rapid testing to the full range of anti-TB drugs and has been applied in a clinical ...bacterial genome in a ...
10
Plantagora: modeling whole genome sequencing and assembly of plant genomes.
... The whole process was run with a perl script, ...the genome identified in ...(reference genome) (assembly sequences) (gff file) ...The genome regions analyzed were: the whole ...
8
Identification of Salmonella for public health surveillance using whole genome sequencing
... in whole genome sequencing (WGS) methodologies have resulted in the ability to perform high throughput sequencing of bacterial genomes at low cost making WGS an economically viable alternative to ...
18
Microbiota present in cystic fibrosis lungs as revealed by whole genome sequencing.
... However, the PCR approach used so far suffers from several limitations and may introduce biases in the observed microbiota compositions. Indeed, selected groups of organisms will be preferentially amplified by the ...
7
Deep Whole-Genome Sequencing to Detect Mixed Infection of Mycobacterium tuberculosis.
... Mixed infection by multiple Mycobacterium tuberculosis (MTB) strains is associated with poor treatment outcome of tuberculosis (TB). Traditional genotyping methods have been used to detect mixed infections of MTB, ...
14
Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences
... [40,41]. Whole- genome sequencing has the potential to determine the full antibiogram if the genetic determinants of antibiotic resistance are known ...loci, whole-genome sequencing has the ...
10
Phylogenetics and differentiation of Salmonella Newport lineages by whole genome sequencing.
... Therefore, whole genome sequencing data was able to provide more accurate phylogenetic relationship than small sets of ...of whole genome sequencing and phylogenetic analysis has been proven ...
12
Quantification of trace-level DNA by real-time whole genome amplification.
... time whole genome amplification method adopting the DOP-PCR strategy is highly stable and accurate for quantification of a wide range of DNA samples from 80 fg to 8 ...one genome-equivalent amounts ...
8
Genomic view of bipolar disorder revealed by whole genome sequencing in a genetic isolate.
... associated with the expression level of genes under linkage peaks (‘eSNPs’ see Methods) [54]. To test for association in the presence of allelic heterogeneity we then performed rareFBAT burden tests for all genes under ...
15
Whole Genome Sequence of Multiple Myeloma-Prone C57BL/KaLwRij Mouse Strain Suggests the Origin of Disease Involves Multiple Cell Types.
... and whole exome and whole genome sequencing of KaLwRij mice, we identified novel KaLwRij gene variants, including deletion of Samsn1 and deleterious point mutations in Tnfrsf22 and ...
13
Enhanced de novo assembly of high throughput pyrosequencing data using whole genome mapping.
... continuous genome coverage across the resulting merged ...draft genome sequence was aligned with the WGRM to identify discordant ...full genome coverage was ...the genome assembly: Firstly, ...
9
Digital Droplet Multiple Displacement Amplification (ddMDA) for Whole Genome Sequencing of Limited DNA Samples.
... Another feature of the ddMDA reactions was identified is the decreased amplification gain. It is likely that the volume restriction in each droplet limits the amplification reaction and decreases the gain. However, the ...
12
Sequence to Medical Phenotypes: A Framework for Interpretation of Human Whole Genome DNA Sequence Data.
... of whole genome sequence data is ...in whole genome sequence data from twelve unrelated adults, and for disease gene discovery in one father-mother-child trio with apparently simplex con- ...
15
Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013.
... understand whole genomic relatedness of the Chinese G3P[8] strains and other G3 RVA strains for which the whole genome data are available, total 121 G3P[8] strains in the world were analyzed by the ...
22
Relationship estimation from whole-genome sequence data.
... detection. Whole-genome sequencing (WGS) marks the final step in increasing genetic marker density by assaying all single-nucleotide variants (SNVs), and thus has the potential to further improve ...
12
Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level
... Comparing both species on a whole genome level (Table 1) reveals new details. S. sobrinus DSM 20742 has seven instead of three genes encoding for GTFs, one which is in- soluble (GTF-I), one intermediate ...
13