Whole Genome Sequencing (WGS)
PulseNet International: vision for the implementation of whole genome sequencing (WGS) for global food-borne disease surveillance
... of whole genome sequencing (WGS) to identify and subtype food-borne bacterial pathogens worldwide, replacing traditional methods to strengthen preparedness and response, reduce global social and ...
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Identification of Salmonella for public health surveillance using whole genome sequencing
... Approximately 8,000 isolates are referred to the Salmonella Reference Service (SRS) at Public Health England (PHE) each year from local and regional hospital laboratories. In April 2015, PHE implemented whole ...
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Whole genome sequencing versus traditional genotyping for investigation of a Mycobacterium tuberculosis outbreak: a longitudinal molecular epidemiological study.
... bacterial genome (blueprint), but because these methods examine only a small part of the genome, they may not distinguish between related transmission ...of whole genome sequencing ...
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Genomic view of bipolar disorder revealed by whole genome sequencing in a genetic isolate.
... obtained whole genome sequences (WGS) for 50 individuals comprising 18 parent-child trios and 8 additional parent-child pairs from the extended pedigree (Figure ...for whole genome ...
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Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach
... conducted whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis on a subset of these isolates for a more in-depth ...
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Microbiota present in cystic fibrosis lungs as revealed by whole genome sequencing.
... explored whole genome sequencing (WGS) using the Illumina high-throughput technology applied directly to DNA extracted from sputa obtained from two cystic fibrosis ...to whole DNA ...
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Deep Whole-Genome Sequencing to Detect Mixed Infection of Mycobacterium tuberculosis.
... Deep whole-genome sequencing (WGS) has been proved highly sensitive and discrimina- tive for studying population heterogeneity of ...the sequencing depth of minor strains was as low as 1× ...
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Retrospective validation of whole genome sequencing-enhanced surveillance of listeriosis in Europe, 2010 to 2015
... routine whole genome sequencing (WGS). Methods: WGS and core genome multilocus sequence typing (cgMLST) were performed on isolates from 2,726 cases from 27 EU/ EEA countries from ...the ...
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Evaluation of whole genome sequencing for outbreak detection of Salmonella enterica.
... essential. Whole genome sequencing (WGS) shows great promises for using as a routine epidemiological typing ...and whole genome sequencing ...
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The "most wanted" taxa from the human microbiome for whole genome sequencing.
... rare sequencing errors and ...or sequencing or PCR ...the sequencing of the most prevalent taxa first represents a rational deployment of sequencing ...for whole genome ...
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Phylogenetics and differentiation of Salmonella Newport lineages by whole genome sequencing.
... study, whole genome sequencing data revealed that strains of ...on whole genome sequencing data by Fricke et ...on whole genome sequencing data provided a ...
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Fast homozygosity mapping and identification of a zebrafish ENU-induced mutation by whole-genome sequencing.
... high-throughput sequencing of the whole zebrafish genome can directly locate the interval carrying the causative mutation and at the same time pinpoint the molecular ...by sequencing the m1045 ...
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Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades
... on whole genome sequencing of the bony-tongued Asian arowana (Scleropages formosus) suggested that the branching of Elopomorpha and Osteoglossomorpha occurred almost simultaneously, placing them as ...
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Insights into the genetic structure and diversity of 38 South Asian Indians from deep whole-genome sequencing.
... targeted sequencing of the mitochondria and Y ...deep whole-genome sequencing with a target coverage of 30X as part of the Singapore Sequencing Indian Project ...Singapore ...
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Identifying Likely Transmission Pathways within a 10-Year Community Outbreak of Tuberculosis by High-Depth Whole Genome Sequencing.
... In total 23 patient isolates were identified with identical MIRU-24 loci and spoligotype (MIRU-12: 23’3425153322; MIRU-24: 242324223342; spoligotype: 777737777740371). The isolate from the earliest clinical case ...
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INSaFLU: an automated open web-based bioinformatics suite "from-reads" for influenza whole-genome-sequencing-based surveillance
... during genome replica- tion or the exchange of genomic segments [ 3 , 4 ...virus genome [ 5 ...next-generation sequencing (NGS) technolo- gies allowing rapid and affordable whole-genome ...
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Tuberculosis: new era for diagnosis and surveillance using whole-genome sequencing-based approaches
... The current study describes the implementation of a WGS-based laboratory workflow for TB surveillance in the Portuguese NRL, in the frame of the demanding supra-national short-term transition from traditional genotyping ...
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Whole genome sequencing and evolutionary analysis of human respiratory syncytial virus A and B from Milwaukee, WI 1998-2010.
... RSV genome, 87–96 different primers were used (Table S1), allowing for the sequencing of 4 complete genomes for a given 384-well ...the sequencing reaction, the plate was pulse centrifuged and ...
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The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs
... When the MiSeq raw sequence data were subjected to analysis using TBProfiler, agreement with phenotypic susceptibility testing was high (95.3%, 82/86; Table 1). Discrepant results were recorded for PZA (×2) and PAS (×2) ...
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Whole genome and exome sequencing of monozygotic twins with trisomy 21, discordant for a congenital heart defect and epilepsy.
... platforms—whole genome sequencing (WGS), duplicate array hybridization (DAH) and RNA sequencing (RNA-Seq), to identify discordant SNVs for monozy- gotic twins discordant for multiple ...Sanger ...
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