[PDF] Top 20 Bioprocessing of human Pluripotent Stem Cells for cardiac cell therapy and pre-clinical research

... autocrine and paracrine effects, and consequently cardiac tissue organization and development (Shimko and Claycomb, ...2008). Cardiac cells experience mechanical strains ... See full document

... any cell-based therapy, the efficacy of this treatment largely depends on the ability of the stem cells to adequately target and distribute throughout tumor ...timing ... See full document

... Advancing pluripotent stem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing ... See full document

... commitment of a specialized cell type from induced pluripotent stem cells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ...progenitor ... See full document

... embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to proliferate indefinitely in an undifferentiated state, and to ... See full document

... Induced pluripotent stem cells (Also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from adult ...introduction ... See full document

... discovery of a simple approach for reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) has revolutionized regenerative medicine ... See full document

... laboratories, and several research groups have reported this culture condition to maintain the undifferentiated growth of hPSCs ...pluripotency of hPSCs was confirmed by teratoma and EB ... See full document

... culture of ASCs the replacement of FBS with pooled allogeneic human serum and human serum derivatives has been reported to support equal or higher proliferation rates and ... See full document

... improvement of 100% (CM monolayers) and 44% (cardiospheres) on metabolic activity recovery was observed when ROCK inhibitor pretreatment was performed using this medium ...enhances cell –cell ... See full document

... The cells exhibited traditional human ES/iPS morphology with a high nuclear to cytoplasmic ratio ...5 cells we routinely used to start reprogramming, the number of human ES like ... See full document

... value of the second iteration, we compared the purity and yield of beta cells isolated based on the CD9/CD56 combination to isolation based on CD9 ...proportion of beta cells ... See full document

... new and better options for men suffering from ED. The introduction of the PDE5i’s drugs revolutionized the manner in which we currently treat ED patients and opened the pathway for further ... See full document

... characterization of MELAS cases is helpful to unveil the still unknown mechanisms and in the search for therapeutic strategies for this ...understanding of mech- anism of mtDNA segregation may ... See full document

... specimens of gingiva (n = 3) were cut into 4–6 mm-thick sections with a cryostat, and the sections placed on slides, air dried and fixed for 10 minutes with 100% ...USA) and the Fc receptor ... See full document

... case of the meganuclease XPCm, we used the epigenetic drug 5aza-dC to demethylate the XPC locus and rescue its nuclease ...enhancement of HGT frequency in 293-H cells (12-fold, 12% HGT, figure ... See full document

... Structural and functional analysis of co-cultured neuronal ...images of co-cultured neurons 48 days after cell plating (bottom ...images of cells and the fluorescence ... See full document

... subtype of type 1 diabetes character- ized by extremely acute and severe insulin insufficiency as a result of almost complete destruction of the pancreatic beta cells even at ... See full document

... myelin-producing cells in vitro that might at least partially contribute to the observed effect of MSC ...MSCs and these cells were washed with PBS before the addition of fresh MMM, ... See full document

... One of the diffi culties with alchemy and this new age of cardiac myocyte biology is related to defi ning a rigorous set of criteria that allow one to make the claim that the cell ... See full document