[PDF] Top 20 BLG ) gene by lentivirus-mediated RNAi
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BLG ) gene by lentivirus-mediated RNAi
... for BLG (Z19569) which span intron(s) to prevent amplification of genomic DNA, using the forward primer (5’-GGC GAG TGT GCT CAG AAG A-3’) and the reverse primer(5’-GGG CTC AGC ACT GTT TTC ...followed by 40 ... See full document
6
Lentiviral-mediated RNAi targeting caspase-3 inhibits apoptosis induced by serum deprivation in rat endplate chondrocytes in vitro
... processes. Gene therapies, using virus vectors or plasmids encoding exogenous proteins to stimulate matrix synthesis or inhibit its degradation, have overcome the limitations of mole- cular treatment ... See full document
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RNAi-mediated knockdown of pituitary tumor-transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
... PTTG1 is highly expressed in a variety of other human tumor tissues (10-12). In addition, PTTG1 can regulate the expression of many genes involved in proliferation and cell cycle control. For example, PTTG1 can activate ... See full document
8
CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
... CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in ...used ... See full document
6
RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
... using gene transfer techniques under the mechanism of ...carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; ...average by more than 51% compared to the con- trol plants (wild ... See full document
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EZH2 depletion blocks the proliferation of colon cancer cells.
... The Enhancer of Zeste 2 (EZH2) protein has been reported to stimulate cell growth in some cancers and is therefore considered to represent an interesting new target for therapeutic intervention. Here, we investigated a ... See full document
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Lentivirus-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth and Induces Apoptosis through MAPK Pathways in Human Retinoblastoma Cells.
... elevated gene-1 (AEG-1) was cloned as a neuropathology-associated gene in primary human fetal astrocytes and has in the meantime emerged as an important oncogene that is markedly overexpressed in multiple ... See full document
14
Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.
... allele-specific gene silencing with mismatched ...indicated by gray bars, in which nucleotide variation and the seed region are indicated by red and blue boxes, ...indicated by green boxes. ... See full document
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Ago-2-mediated slicer activity is essential for anti-flaviviral efficacy of RNAi.
... replication by binding to viral coding region or 39UTR, our results suggest that synthetic siRNAs designed to mimic miRNA having a seed match to the viral sequences may not be enough for antiviral activity in an ... See full document
6
RNAi-mediated silencing of the Bmi-1 gene causes growth inhibition
... quantified by spectrophotometry at 260 ...followed by 30 re- action cycles at 94 °C for 30 s, 51 °C for 30 s, 72 °C for 30 s, and a final extension cycle at 72 °C for 5 ...analyzed by using Vistra ... See full document
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Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
... Protein phosphatase magnesium/manganese-dependent 1D (PPM1D), also called wild-type p53-induced phospha- tase (Wip1), is a member of the magnesium-dependent serine/threonine protein phosphatase (PPM) family (6,7). It was ... See full document
6
Signalling pathways involved in adult heart formation revealed by gene expression profiling in Drosophila.
... Transcription factors dynamically expressed during the heart remodelling process. The first column corresponds to the expression matrix specifically constructed from transcription factors signifi- cantly regulated during ... See full document
15
Resistance of renal cell carcinoma to sorafenib is mediated by potentially reversible gene expression.
... week by gavage beginning when the tumors had grown to a diameter of 12 mm as previously described ...Growth by 2 mm from that size is the minimal reproducible growth that can be accurately measured ... See full document
10
Dissecting mitosis by RNAi in Drosophila tissue culture cells
... after RNAi in Drosophila tissue culture cells can be complex since the penetrance of RNAi is not absolute, these cells are hard to synchronize, live cell analysis is not yet well developed and the resulting ... See full document
9
As Mediated by Communications Institutions
... uenced by the costs of acquiring and maintaining radio and television equipment or even paying for copies or subscriptions to newspapers, magazines and now electronic services including the ... See full document
26
Centromere-independent accumulation of cohesin at ectopic heterochromatin sites induces chromosome stretching during anaphase.
... mechanism by which chromo- somal translocations involving long stretches of heterochromatin may induce mitotic errors, as the abnormal chromatin stretching could potentially be problematic to the ...on gene ... See full document
16
Molecular insights into reprogramming-initiation events mediated by the OSKM gene regulatory network.
... performed by using the Nucleofector II (Lonza, Basel, Switzerland) and Nucleofector Kit R / program U- ...harvested by trypsinization and counted cell pellets consisting of 5610 5 cells were resuspended in ... See full document
17
Fine‐scale genetic structure and flowering output of the seagrass Enhalus acoroides undergoing disturbance
... The change of genetic diversity is not always pronounced as a response to disturbance, because even low levels of gene flow can significantly alleviate the loss of genetic diversity. However, SGS appears to be ... See full document
10
Long astral microtubules and RACK-1 stabilize polarity domains during maintenance phase in Caenorhabditis elegans embryos.
... In embryos that had shorter astral microtubules and were defective in polarity maintenance, we observed shifts of cortical domains. The boundaries were no longer perpendicular to the AP axis. However, we did not observe ... See full document
9
Chloroquine mediated modulation of Anopheles gambiae gene expression.
... on gene expression in the mosquito host by conducting a genome-wide transcript ...mosquito gene expression after exposure to chloroquine under both naı¨ve and malaria- infected ... See full document
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