[PDF] Top 20 Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
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Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
... Culling of seroreactive dogs from AVL-endemic areas for the control of the disease is a polemic subject because of the disagreement among investigators regarding the ... See full document
4
Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin- embedded healthy skin for the diagnosis of canine visceral leishmaniasis
... limitations and increase the sensitivity of the PCR technique for detection of the parasite genome in FFPE samples, the use of a larger number ... See full document
8
Molecular diagnosis of canine visceral leishmaniasis : a comparative study of three methods using skin and spleen from dogs with natural Leishmania infantum infection.
... (PCR) and its variations represent highly sensitive and specific methods for Leishmania DNA detection and subsequent canine visceral leishmaniasis (CVL) ... See full document
6
Detection of immunoglobulin G in the lung and liver of hamsters with visceral leishmaniasis
... tum in Goodpasture syndrome (10) and in acute and chronic immune complex-medi- ated disease models ...(11). In the present study, we observed the presence of ... See full document
5
Seroprevalence and risk factors for canine visceral leishmaniasis in the endemic area of Dias D'Ávila, State of Bahia, Brazil
... means of ELISA, in accordance with the standardized protocol of ENSP, FIOCRUZ, Rio de Janeiro (the reference laboratory for leishmaniasis diagnosis), with some ...(1µg/well) ... See full document
5
Immunohaematological and rheological parameters in canine visceral leishmaniasis
... assessed. The rheological properties of the blood samples were also ...haematocrit and platelet values of dogs in the CVL group were found to be lower than those ... See full document
7
Surveillance of canine visceral leishmaniasis in a disease-free area
... veterinarians of the Center for Zoonosis Control following the standard protocol and was not related to the present ...blood samples were then collected by intracardiac punction, ... See full document
3
Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations
... number of studies have considered the role of the domestic dog in the transmission of HVL (Silva et ...2004). The most significant finding to emerge from such ... See full document
7
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
... detected in the lymph nodes, some (10/35) still maintained a negative serology, suggesting that this parameter is not appropriate for diagnosis of CVL while the detection of ... See full document
11
Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil
... This study aimed to verify the occurrence of Leishmania spp. and Leishmania (Leishmania) infantum in horses from a visceral leishmaniasis endemic area in ...DNA ... See full document
6
Canine visceral leishmaniasis: Diagnosis and management of the reservoir living among us
... serodiagnosis. Methods that use crude promastigote antigens are highly sensitive for detecting subclinical and clinical infections but have lower specificity than methods that employ other antigens ... See full document
13
Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis
... aim of the present study was to evaluate the serological methods using ELISA with recombinant-rK39 (ELISA-rK-39) and soluble extract-SE (ELISA-SE) antigens, the indirect ... See full document
8
Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis
... 1:200 in PBS with 2% normal rabbit ...80 and incubated with 1:4000 of alkaline phosphatase con- jugated to anti-dog IgG (Sigma) for 1 h at ...washed and 100 µL diethanolamine solution ... See full document
5
USE OF ELISA EMPLOYING HOMOLOGOUS AND HETEROLOGOUS ANTIGENS FOR THE DETECTION OF IgG AND SUBCLASSES (IgG1 AND IgG2) IN THE DIAGNOSIS OF CANINE VISCERAL LEISHMANIASIS
... is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is ...for ... See full document
8
The use of conjunctival swab samples for PCR screening for visceral leishmaniasis in vaccinated dogs
... between the genes coding for SSU rRNA and 5.8S rRNA were used. For the first amplification, ...µL of DNA solution was added to 40.0 µL of PCR mix containing 15 pmol of the ... See full document
6
Study of Leishmania Antigens for the Diagnosis of Human Visceral Leishmaniasis
... epitope of the 230kDa kinesin related protein of ...sensitivity of 93.5% and a specificity of ...(70). The InBios Kalazar Detect rapid test utilizes the recombinant ... See full document
35
Use of domperidone in canine visceral leishmaniasis: gaps in veterinary knowledge and epidemiological implications
... nificantly the risk of treated dogs to transmit ...against the parasite, it sub-utilization would not carry the risk of developing drug resistance of ...infantum. In ... See full document
4
Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis
... on the LN immunophenotypic profile during ...that the LN lymphocyte population of ...increase in Thy-1 + and CD5 + T-cells, particularly CD8 + T-cells, when com- pared to uninfected ... See full document
11
Clinical Forms of Canine Visceral Leishmaniasis in Naturally Leishmania infantum –Infected Dogs and
... genesis of hematological alterations in both red and white blood cell series is often related to bone marrow disorders such as dysplasia and aplasia ...examination of bone marrow ... See full document
9
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
... presence of granulomas was observed in Leishmania infection (Abranches personnal communication) its presence in this study, in one fox, does not exclude infection due to other patho- ... See full document
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