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[PDF] Top 20 Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

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Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

... one of the most deadly and disabling, disease in the developing ...cause of death and the tenth highest cause of dis- ...burden of tuberculosis is ... See full document

4

DETECTION OF MYCOBACTERIUM BOVIS DNA IN NASAL SWABS FROM TUBERCULOUS CATTLE BY A MULTIPLEX PCR Eduardo Eustáquio de Souza Figueiredo

DETECTION OF MYCOBACTERIUM BOVIS DNA IN NASAL SWABS FROM TUBERCULOUS CATTLE BY A MULTIPLEX PCR Eduardo Eustáquio de Souza Figueiredo

... Bovine tuberculosis infection in cattle is usually diagnosed in the live animal based on delayed hypersensitivity reactions (intradermal tuberculin tests), which may lack both sensitivity and ... See full document

5

Detection of tuberculosis drug resistance a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype MTBDRplus

Detection of tuberculosis drug resistance a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype MTBDRplus

... M. tuberculosis lineage is pro- vided by this ...characteristics in a single step are appealing and the multiplex ligation-dependent probe amplification (MLPA) technique is able to achieve the ... See full document

8

EVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND URINE SAMPLES Heidi Lacerda Alves da Cruz

EVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND URINE SAMPLES Heidi Lacerda Alves da Cruz

... presence of DNA of M. tuberculosis since it amplifies genetic material derived from dormant/nonviable ...Cases of patients with infection by mycobacteria are not diagnosed as soon as needed so ... See full document

9

Plasmidbased controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reactionhighresolution melting

Plasmidbased controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reactionhighresolution melting

... discrimination of the melting curve profiles of pGEM-C526T and pGEM-C531T was difficult due to the nucleotide change being the same and the proximity of the mutations in the DNA ... See full document

4

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

... negative PCR. Unfortunately, the PCR results could not be confirmed because these samples had been ...Both of these samples were obtained from patients who had cultured positive for MTBC within the ... See full document

6

Role of a GenoType MTBDRplus line probe assay in early detection of multidrug-resistant tuberculosis at a Brazilian reference center

Role of a GenoType MTBDRplus line probe assay in early detection of multidrug-resistant tuberculosis at a Brazilian reference center

... to Mycobacterium tuberculosis is a reality worldwide, and its diagnosis continues to be difficult and time ...use of rapid molecular tests. We evaluated the routine use (once a week) ... See full document

6

Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

... Spoligotyping and database comparison - A loop- ful of culture was suspended in tris-ethylenediamine tetraacetic acid (EDTA) (TE) buffer [10 mM Tris-HCl, 1 mM EDTA (pH ...8.0)] and ... See full document

7

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn’s disease

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn’s disease

... Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to ...value of ... See full document

5

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

... the detection of Plasmo- dium are easy to use and can produce fast results, but they usually have lower sensitiv- ity and specificity than conventional micro- scopic ...Many of them ... See full document

9

J. bras. pneumol.  vol.41 número6

J. bras. pneumol. vol.41 número6

... costs of a molecular test for Mycobacterium tuberculosis and resistance to rifampin (Xpert MTB/RIF) and of smear microscopy, within the Brazilian Sistema Único de Saúde ... See full document

4

PDF EN Jornal Brasileiro de Pneumologia 6 9 english

PDF EN Jornal Brasileiro de Pneumologia 6 9 english

... costs of a molecular test for Mycobacterium tuberculosis and resistance to rifampin (Xpert MTB/RIF) and of smear microscopy, within the Brazilian Sistema Único de Saúde ... See full document

3

Effects of tissue handling and processing steps on PCR for detection of Mycobacterium tuberculosis in formalin-fixed paraffin-embedded samples

Effects of tissue handling and processing steps on PCR for detection of Mycobacterium tuberculosis in formalin-fixed paraffin-embedded samples

... fixation and paraffin embedding: Fresh tissue specimens of spleen and lung from an AIDS patient, who died from miliary tuberculosis, strongly positive for acid-fast bacilli, were subjected to ... See full document

6

J. bras. pneumol.  vol.39 número6

J. bras. pneumol. vol.39 número6

... performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and ... See full document

8

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

... culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine ...as PCR can provide fast and reliable results while significantly decreasing the ... See full document

8

J. bras. pneumol.  vol.37 número3 en v37n3a04

J. bras. pneumol. vol.37 número3 en v37n3a04

... X-rays of patients with pulmonary tuberculosis and to determine whether the extent of radiographic lesions correlates with bacteriological ...Methods: In this retrospective, descriptive ... See full document

8

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

... specificity of the nested-PCR was ana- lyzed in ...DNA of the atypi- cal mycobacteria ...present in lymph nodes submitted for diagnostic testing can con- found assays that lack ... See full document

8

Rev. Soc. Bras. Med. Trop.  vol.46 número5

Rev. Soc. Bras. Med. Trop. vol.46 número5

... performance of an in-house nested-PCR system for the detection of the Mycobacterium tuberculosis complex in pleural fl uid, blood and urine samples from ... See full document

6

EVALUATION OF METHODS FOR DETECTION AND IDENTIFICATION OF MYCOBACTERIUM SPECIES IN PATIENTS SUSPECTED OF HAVING PULMONARY TUBERCULOSIS

EVALUATION OF METHODS FOR DETECTION AND IDENTIFICATION OF MYCOBACTERIUM SPECIES IN PATIENTS SUSPECTED OF HAVING PULMONARY TUBERCULOSIS

... (SUS) in Brazil, diagnosis of TB is routinely based on baciloscopy results (4), but in specific cases, cultures are performed at some of the few Central Laboratories of Public Health, ... See full document

6

Comparison of real-time PCR and conventional PCR for detection of

Comparison of real-time PCR and conventional PCR for detection of

... available in several laboratories, especially in the private sector; however, the interpretation of its results requires new levels of ...accuracy of the method (31). In addition ... See full document

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