[PDF] Top 20 Detection of multiple mycoplasma infection in cell cultures by PCR
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Detection of multiple mycoplasma infection in cell cultures by PCR
... total of 301 cell cultures from 15 laboratories were monitored for mycoplasma (Mollicutes) using PCR and culture ...The infection was detected in the cell culture ... See full document
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Nested-PCR for the detection of Mycoplasma hyopneumoniae in bronchial alveolar swabs, frozen tissues and formalin- ixed paraf in-embedded swine lung samples: Comparative evaluation with immunohistochemical indings and histological features
... collections of FFPE samples, allowing important retrospec- tive studies using histological analysis (Ferrer et ...isolation of the nucleic acids but also introdu- ces polymerase “blocks” during PCR ... See full document
6
Prevalence of ‘Candidatus Mycoplasma haemobos’ detected by PCR, in dairy cattle from Ji-Paraná in the north region of Brazil
... municipality of Ji-Paraná, Rondônia, is one of the major dairy production areas in the north region of ...productivity in the industry. The objective of this study was to ... See full document
6
Development and evaluation of a sensitive PCR-ELISA system for detection of schistosoma infection in feces.
... The detection limit of the Schistosoma PCR-ELISA system was determined to be ...fg of S. mansoni genomic DNA (less than the amount found in a single cell) and estimated to be ... See full document
8
A VP3VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples
... VP1 PCR (Todd et al., 1992) was reported for the detection o CAV in MSB-1 infected cultures and SPF chickens experimentally inoculated with a controlled virus titer of the known ... See full document
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DETECTION OF MYCOPLASMA PULMONIS IN LABORATORY RATS
... diagnosis of MRM is not difficult. However, there are difficulties in diagnosing assymptomatic animals, as well as in establishing the differentiation among pathogenic and commensal ...on ... See full document
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Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
... diagnosis of mycoplasma recom- mended for the monitoring of mycoplasmosis in poultry farms for the detection of antibodies in sera are the rapid serum agglutination, that ... See full document
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Characterization of a novel calicivirus causing systemic infection in atlantic salmon (Salmo salar L.): proposal for a new genus of caliciviridae.
... variants of ASCV were ...passages of fish cells originally repeatedly inoculated with heart tissue homogenates from salmon suffering from ...splitting of inoculated cells and repeated boosting with ... See full document
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Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
... Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the ... See full document
6
Detection of Pneumocystis in lungs of bats from Brazil by PCR amplification
... species of unusual, ascomyceteous yeasts, each of which can colonize the lungs of a different mammal (Stringer ...spp infection has been reported in many mammalian hosts, such as ... See full document
5
DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.
... day in ICU using the Simplified Acute Physiology Score (SAPS II) [41], and Sepsis-related Organ Failure Assessment (SOFA) score ...absence of infection was documented according to the recently ... See full document
7
Detection of cytomegalovirus infections by PCR in renal transplant patients
... study in Brazil to use PCR to detect CMV infection in renal transplant ...DNA in 64.9% of the transplanted patients. The findings of Rowley et ...the PCR assay is ... See full document
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Genetic and biological characterization of a densovirus isolate that affects dengue virus infection
... detected in the super- natant of C6/36 cells infected with YFV (BR/01) that had been sent to our laboratory for research purposes (Supplementary ...Because of the atypical cytopath- ic effect that ... See full document
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Detection of Mycoplasma hyopneumoniae in lungs and nasal swabs of pigs by nested PCR
... Micoplasmal infection causes pneumonia characterized by a sporadic, dry and non- productive cough, retarded growth rate, and inefficient utilization of feed (Ross, ...caused by bacterial ... See full document
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MULTIPLE ORGAN DYSFUNCTION SYNDROME ASSOCIATED WITH MYCOPLASMA PNEUMONIAE INFECTION Shu-Bo Zhai
... numbers of the pathogen found in patient ...identification of the infection ...MP infection (9) although nucleic acid amplification tests (NAATs) on throat secretions are superior ... See full document
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Prevention and Detection of Mycoplasma Contamination in Cell Culture
... period of 2 weeks. MSCs from Wistar rats were separated and cultured in Dulbecco’s PRGL¿HGHDJOHPHGLXP '0(0 7KHFRQGLWLRQPHGLXP &0 ZKLFKFRQWDLQHGIDFWRUVVHFUHWHGE\ ... See full document
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A SNAP-tagged derivative of HIV-1--a versatile tool to study virus-cell interactions.
... levels of released virus were reached at 18 days post ...introduction of an additional PR cleavage site between the MA and SNAP moieties (HIV iSNAP , triangles) did not result in increased ... See full document
9
MARISA DE CACIA OLIVEIRA1
... treatment of 9-day-old cultures of ...on cell growth (figure 2A). The quantification of anthraquinones using the molar extinction coefficient of alizarin revealed that, under our ... See full document
6
Assessments of total and viable Escherichia coli O157:H7 on field and laboratory grown lettuce.
... inoculation in the growth chamber, we were able to more closely mimic the rapid decline in ...inoculation of this organism onto lettuce plants in the Salinas Valley ...majority of E. ... See full document
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ASSESSMENT OF PCR IN THE DETECTION OF
... shown in Table 2, using whole ...present in 87% of the test samples, respectively. The possibility of false-negative results due to the presence of any inhibitory contamination ... See full document
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