[PDF] Top 20 Detection of antibodies using Ab-ELISA
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Detection of antibodies using Ab-ELISA
... Use of MoAb-TS14 for the detection of circulating antigen ...minutes of centrifugation (10000g), the supernatant was neutralized using the same vo- lume of a ...well of ... See full document
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Padronização de ELISA indireto para a detecção de anticorpos anti-Leptospira em soros bovinos / Indirect ELISA standardization for the detection of antibodies anti-Leptospira in bovine serum
... cause of the ...diagnosis of leptospirosis, but it has ...indirect ELISA, using serovar Hardjo as antigen, was compared to the MAT, in an initial evaluation for the individual diagnosis ... See full document
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Improved Methods for the Diagnosis of African Trypanosomosis
... antigen detection methods. These revolve around the nature of the antigen itself in a serum sample, the collection and processing of the sample and the biology of the trypanosome-host interac- ... See full document
6
An enzyme-linked immunosorbent assay (ELISA) for the detection of IgM antibodies against Leishmania chagasi in dogs
... areas of Latin America, Europe, Africa and Asia and is increasingly becoming a major public health problem (WHO ...prevalence of human and canine AVL has been reported in Brazil, not only in terms of ... See full document
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Identificação do biomarcador NS1 na saliva como diagnóstico da dengue
... antigen detection in saliva samples using ELISA ...total of 44 saliva samples were obtained from November 2012 to February ...NS1- ELISA presented a sensibility of ...NS1 ... See full document
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Padronização de Dot-ELISA para detecção de anticorpos anti-Leptospira em soro bovino / Dot-ELISA standardization for the detection of antibodies anti-Leptospira in bovine serum
... O MAT é considerado o teste sorológico padrão-ouro para o diagnóstico da leptospirose em humanos e animais [3]. Entretanto, os resultados mostraram que o Dot-ELISA no formato proposto foi capaz de detectar ... See full document
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Evaluating HIV Prevention Programs: Herpes Simplex Virus Type 2 Antibodies as Biomarker for Sexual Risk Behavior in Young Adults in Resource-Poor Countries.
... total of 785 students (413 girls, 372 boys) were randomly sampled from the 20,000 students enrolled in grades 7 and 8 in 2003 ...64% of sampled students were found, contacted in their homes and asked to ... See full document
11
Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli: Production and Use in Immunodiagnosis
... anti-rabbit antibodies-alkaline phosphatase conjugates (Sigma), and fusion proteins were visualized by reaction with NBT/BCIP as a ...ASGV-antibodies, using extracts from non-transformed ... See full document
5
Comparison of dot-ELISA and standard ELISA for detection of
... monoclonal antibodies against LPS deter- minants (WBE12-C10-C10, L3,7,9-specific; 6E7-10, L8- specific;and3G3-1-8C,L1-specific;allgenerouslysupplied by ...Institute of Research, ... See full document
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CIRCULATION OF ANTIBODIES AGAINST YELLOW FEVER VIRUS IN A SIMIAN POPULATION IN THE AREA OF PORTO PRIMAVERA HYDROELECTRIC PLANT, SÃO PAULO, BRAZIL
... objectives of the present study were: a) to standardize immunoenzymatic assay (ELISA) procedures for the detection of IgG antibodies in monkey sera samples, and b) to estimate the ... See full document
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MATERIALS AND METHODS Bacteria
... panel of 13 MAbs was obtained according to procedures recommended by Goding ...mice of three to four weeks of age were immunised via intraperitoneal with the OMP from Salmonella ...end of the ... See full document
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Antileishmanial IgG and IgE Antibodies Recognize Predominantly Carbohydrate Epitopes of Glycosylated Antigens in Visceral Leishmaniasis
... wells of ELISA plates and their carbohydrates oxidized to aldehyde with 100 µl of 10 mM sodium metaperiodate in 50 mM acetate buffer, pH ...µl of 50mM sodium boro- hydride in PBS (Woodward et ... See full document
6
Detection of helicobacter pylori in nasal polyps using rapid urease test and ELISA
... 23- Yilmaz T, Ceylan M, Akyony Ozcakir O, Gursel B. The role of Helicobacter pylori in otitis media with effusion. European society of clinical microbiology and infections disease. [cited 2006]. Available ... See full document
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Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
... specificity of 75 and 97.75%. Previous studies detecting anti-HCV antibodies in oral fluid resulted in a sensitivity of 72% and specificity 98% using Salivettes collection device with Ortho ... See full document
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Toxoplasma gondii antibodies sheep in Lages, Santa Catarina, Brazil, and comparison using IFA and ELISA
... mainly of animals aged ≥ 13 months ...likelihood of coming into contact with ...state of Paraná; Carneiro et ...region of the state of ... See full document
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Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens
... frequently using the ARAV antigen as we did when using the MACV anti- ...phase of infection, but had no symptoms or apparent ...evidence of disease is present when IgM antibodies are ... See full document
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Detection of specific IgE antibodies in parasite diseases
... presence of specific IgE ...picture of visceral leishmaniasis and the diagnosis was confirmed by demonstra- tion of amastigote forms in material obtained from bone marrow or spleen aspiration and ... See full document
5
MAJOR-INFECTION IN MICE BY NORMAL POLYSPECIFIC
... Increasing of leishmaniasis incidence around the world represents a serious problem of public ...cases of leishmaniasis are diagnosed every year and more than 80% of the total 12 million cases ... See full document
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Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves.
... DAS ELISA perfor- mance. The FMD vaccines consisted of either crude inactive viral antigens or half concentrated antigens; therefore, crude inactive viral antigens were chosen as the standard antigen in ... See full document
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Short Communication Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
... chaotrope ELISA, the standardization of the optimal concentration of dissociating agents (chaotropes) was made by using the 20 serum samples previously assayed for both ...standardization ... See full document
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