[PDF] Top 20 Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
... the assay correctly identified 90.6% RIF-resistant strains. In detail, one isolate with the 529 (CGA→CAA) mutation showed weak hybridization signal at the Rif4 and a strong one at the ...end ... See full document
7
First evaluation of drug-resistant Mycobacterium tuberculosis clinical isolates from Congo revealed misdetection of fluoroquinolone resistance by line probe assay due to a double substitution T80A-A90G in GyrA.
... laboratory of bacteriology from the teaching hospital at Lille (France) for culture and determination of first-line drug susceptibility of isolates as well as mutations profiles associated with the ... See full document
6
Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction- single-strand conformation polymorphism and direct sequencing methods
... those of Bahremand et ...poorly in detecting mutations in rpoB re- gion. However, in this study, 1/21 ...%) of the resistant isolates had a PCR-SSCP pattern similar to that ... See full document
6
Low-stringency single-specific-primer PCR as a tool for detection of mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis
... economies of developing countries since the disease generally strikes in- dividuals in their prime working ...Resistance of Mycobac- terium tuberculosis to antituberculosis drugs is a ... See full document
3
Detection of tuberculosis drug resistance a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype MTBDRplus
... advantage of being easily optimised on a routine basis to address accurately the early detection of MDR-TB, with the pos- sibility to expand to further drug targets and ...antibiotics. In ... See full document
8
Direct Detection by the Xpert MTBRIF Assay and Characterization of Multi and Poly Drug- Resistant Tuberculosis in Guinea-Bissau, West Africa
... molecular detection of Mycobacterium tuberculosis complex In October 2012, one GeneXpert instrument equipped with four modules was installed for the first time in Guinea-Bissau, ... See full document
14
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis
... Mutations in katG315 are associated with a higher minimum inhibitory concentration for INH than muta- tions in the inhA promoter region and therefore are prob- ably of greater clinical significance ... See full document
5
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
... described by Hermans et al. (1990). PCR was performed in a final volume of 50 µL, containing ...each of dATP, dCTP, dGTP and dTTP, 10 mM Tris-HCl (pH ...pmoles of each primer (INS-1 and ... See full document
6
Fast test for assessing the susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by real-time PcR
... Mycobacterium tuberculosis is the bacterium that causes tuberculosis (TB), a leading cause of death from in- fectious disease ...diagnosis of resistant strains is ... See full document
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Role of a GenoType MTBDRplus line probe assay in early detection of multidrug-resistant tuberculosis at a Brazilian reference center
... M. tuberculosis complex, marked as TUB in the ...composed of wild-type (WT) probes that included the most important resistance areas of the referred genes (rpoB WT 1 to 8, KatG WT, and inhA WT ... See full document
6
Mycobacterium tuberculosis MLPA assay versus Genotype
... advantage of being easily optimised on a routine basis to address accurately the early detection of MDR-TB, with the pos- sibility to expand to further drug targets and ...antibiotics. In ... See full document
8
Detection Of Hepatitis B Virus DNA In Moroccan Patients With Epithelial Ovarian Carcinoma EOC By Polymerase Chain Reaction
... occurred in both surgical and chemotherapeutic techniques, the epithelial ovarian cancer remains the leading cause of death from gynecological malignancy in the world with 5-year survival rates lower ... See full document
3
Estudo da interação da 2-trans-Enoil-ACP (CoA) Redutase de Mycobacterium tuberculosis com o complexo inorgânico Isoniazida-pentacianoferrato por simulação pela dinâmica molecular
... occur in the substrate binding loop [26], comprehending helix α6 and α7, and that the overall Rossman fold [50] of the enzyme remains ...is in agreement with the X-ray diffraction studies of ... See full document
91
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity
... aliquot of 300 µL of 7H9 cultures was removed to a sterile tube containing 2 mL of sterile 22 mmol/L phosphate buffer and 10 mmol/L sodium nitrate and incubated at 35-37 ºC for 2 ...µL of ... See full document
7
J. bras. pneumol. vol.37 número3 en v37n3a04
... limitation of the present study is its retrospective ...care of male laborers and their male relatives, all results were derived from the records of those ...power of the ...investigated ... See full document
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Evaluation of four colourimetric susceptibility tests for the rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates
... concentration of drugs and in liquid ...defined in the lit- erature. Each bacterial isolate was tested in each of the three wells containing INH ...each of the susceptibility ... See full document
6
Genome Analysis of the First Extensively Drug-Resistant (XDR) Mycobacterium tuberculosis in Malaysia Provides Insights into the Genetic Basis of Its Biology and Drug Resistance.
... M. tuberculosis acquires drug resistance through the sequen- tial acquisition and accumulation of resistance mutations in the chromosome during sub-opti- mal drug treatment instead of ... See full document
20
ANTIMYCOBACTERIAL ACTIVITY OF THE ANTIINFLAMMATORY AGENT DICLOFENAC SODIUM, AND ITS SYNERGISM WITH STREPTOMYCIN
... effects of combination of Dc plus Sm proved synergism, which could be further substantiated by carrying out tests for FIC ...been in use satisfactorily in clinical medicine with known ... See full document
8
J. Braz. Chem. Soc. vol.21 número7
... M. tuberculosis strains were recorded after 5-7 days of incubation at 37 °C, and the MIC values reported here represent an average of three tests carried out ...manipulation of biological ... See full document
6
Decreased phenotypic susceptibility to etravirine in patients with predicted genotypic sensitivity.
... mL of plasma from nine samples (QIAamp Viral RNA Mini Kit, QIAGEN, Valencia, CA) after centrifugation at 9,0006g for 1 ...end of the reverse transcriptase region was reverse transcribed and ... See full document
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