[PDF] Top 20 Extraction of biomolecules with aqueous two phases systems

... ability of inorganic salts to promote the phase separation and, consequently, a salting-out effect follows the order: K3PO4 > K2HPO4 ≈ K2CO3 > ...anions with a higher valence (PO4 3- ) are better ... See full document

... one of the most important their tailoring ability, ionic liquids (ILs) have been investigated in several fields and proposed as alternatives to hazardous volatile organic compounds commonly used by ...use ... See full document

... behavior of natural dye carmine and norbixin in different ATPS systems formed from polymer and ...transfer of dyes to the upper phase of the ATPS was observed and thus demonstrating the ... See full document

... g of salt and 300 g of polymer were weighed out (BL3200H, Martei, uncertainty of ...vat with a capacity of 3.0 L. About 1900 g of the ef fluent was added to the system using a ... See full document

... and/or phases' separation mechanisms can be ...effect of impurities on both the solute partition and phases ’ equilibrium and compositions should be ...purity of the phase- forming components ... See full document

... development of alternative downstream ...associated with the most traditional ATPS have been identified, such as the high cost of some polymers allied to the high viscosities of the ... See full document

... view of the importance of resource recycling and the use of environmentally safe systems to extract the metals contained in wastes, the present study concerns the extraction/separation ... See full document

... the extraction of aminoacids from an aqueous phase using hydrophobic water immiscible ...magnitudes of L-tryptophan partition coefficients using IL-based ABS can be as much as 1000 times ... See full document

... composed of a large organic cation and a small organic or inorganic anion, conferring them an asymmetrical ionic structure and higher temperatures of ...one of the most interesting properties ... See full document

... number of studies regarding the phase behaviour of ternary systems composed of ILs + water + inorganic salts has been reported [56, 58, ...One of these studies showed that the influence ... See full document

... processes, aqueous biphasic systems (ABS) have been recognized as an economic and efficient downstream processing tool to be used in the separation and purification of biomolecules from ... See full document

... ciency of phenolic compounds extraction decreases as temperature and concentration of solvent ...results of this study are similar to those of other authorsś Venditti et ...for ... See full document

... factor of 2.2- fold (purity of 1.24) was obtained with a composition of 4% of polymer and 21% (w/w) of the ...maximization of experimental conditions was achieved and this ... See full document

... decade, aqueous biphasic systems (ABS) composed of ILs and a wide number of salts, carbohydrates, polymers, or amino acids have been extensively studied for the extraction and puri ... See full document

... range of polarities between the two phases [20], thus hampering their effective application in the purification of target proteins from complex ...functionalization of PEG with ... See full document

... use of imidazolium-based ILs in polymer-salt systems as a way to increase the purification factors of a clinically relevant enzyme - ...hydrophobicity of this phase, resulting in a decrease ... See full document

... effect of the polymer molecular weight and temperature on the ...coefficient of the protein increases with decreasing ...form of heat, the internal energy rises, increasing the average kinetic ... See full document

... weights of the ATPSs prepared were ...minimum of 12 h in a ther- mostatic water ...bottom phases were collected separately using ...solubility of the dye in the polymer phase, simu- lating an ... See full document

... IL-rich phases composed by halogenated ions, such as Cl − or Br − , or for ILs comprising anions with a more hydrophobic character and a higher hydrogen bonding accepting character, such as [N(CN) 2 ] − ... See full document

... kind of compounds are sensitive to water hardness, ...(e.g. with a quaternary ammonium ...charge of the hydrophilic ...surfactants with a non-charged hydrophilic region ...combination ... See full document