[PDF] Top 20 Extraction of phenolic compounds with aqueous two-phase systems

... The extraction of a molecule with ATPS strongly depends on the ability to manipulate the physical/chemical properties of the phases aiming at obtaining high partition coefficients and specific ... See full document

... solubilization of the target ...solubilization of IFNα-2b from inclusion bodies, and general guidelines provided by Palmer and Wingfield [44], an optimized strategy towards the efficient solubilization ... See full document

... concentration of total proteins in the enzyme extract was estimated by spectrophotometry using the method based on adsorption of the reagent Coomassie Brilliant Blue G-250 as proposed by Marion Bradford ... See full document

... Aqueous two-phase systems (ATPSs) offer an excellent alterna- tive for the selective extraction of metals in hydrometallurgical ...for phase splitting, without stable ... See full document

... use of ATPS to prepare samples for the separation of iron ...selective extraction of Fe(III) in the presence of ...polymer-rich phase with the help of the ... See full document

... knowledge of protein behaviour in aqueous-two phase systems will also lead to the ability to predict the partitioning of specific materials (Silva and Franco, ...interpahse ... See full document

... factor of 2.2- fold (purity of 1.24) was obtained with a composition of 4% of polymer and 21% (w/w) of the ...maximization of experimental conditions was achieved and this ... See full document

... bioactive compounds, the flavonoids are efficient therapeutic molecules with several pharmacological properties recognized for the scientific ...maintenance of central nervous, cardiovascular, ... See full document

... g of salt and 300 g of polymer were weighed out (BL3200H, Martei, uncertainty of ...vat with a capacity of 3.0 L. About 1900 g of the ef fluent was added to the system using a ... See full document

... composed of large ions that cannot easily form an ordered crystal and thus remain liquid at or near room ...knowledge of their mutual solubilities with water is very important for the ... See full document

... behavior of natural dye carmine and norbixin in different ATPS systems formed from polymer and ...transfer of dyes to the upper phase of the ATPS was observed and thus demonstrating the ... See full document

... use of ATPS for different purposes [ 3 ...understanding of the phase separation and partition ...in two categories: ATPS used in downstream processing operations ...separation, ... See full document

... concentrations of alcohol and salt (16/16, 18/20, 20/20 and 24/22 by mass) were tested to evaluate the potential use of alcohol-based ATPS, 20% (by mass) of enzyme exctract and deionized water to ... See full document

... result of the high affinity between amino acids and RNA 27 , amino-acid-based affinity chromatography is highly used to purify this biopolymer 10, 28,29 ...together with the fact that in general ... See full document

... equipped with a quadrupole instru- ment fitted with an electrospray source in the positive and nega- tive ESI modes and atmospheric pressure chemical ionization ...operated with the following ... See full document

... best of our knowledge, we report here, for the first time, the remarkable ability of IL- based ATPS to extract textile dyes in a single-step procedure with extraction efficiencies up to 100 ... See full document

... model aqueous two-phase system were conducted to find out if apigenin and apigenin-7-O-β-glucoside show affinity to the top and the bottom phase, respectively, ...possibility of their ... See full document

... ILs with higher octanol-water partition coefficients - [Ch] [Abt], [Ch][C3C] and [Ch][Gen] were not able to form an ATPS with PPG 400, although an exception to this pattern was ...precipitation of ... See full document

... each phase diagram. The total weights of the ATPSs prepared were ...minimum of 12 h in a ther- mostatic water ...top phase as ...solubility of the dye in the polymer phase, simu- ... See full document

... introduction of ILs to functionalize PEG (101), in others works, small quantities of ILs (as adjuvants) were added into conventional polymer-salt-ABS to tailor the extraction and selectivity ... See full document