[PDF] Top 20 Functional characterization of peripheral blood dendritic cells and monocytes in systemic lupus erythematosus

... that monocytes differentiate also into DCs in vivo [ 9 , 10 ...differentiation of DCs from a pre-monocyte precursor or monocytes remains con- ...population, and at least three different ... See full document

... from peripheral blood mono- nuclear cells (PBMCs) with TRIzol (Invitrogen, USA) and first-strand cDNA was synthesized by using an RT system (Promega, ...assessment of DNA ampli- fication ... See full document

... cycle of 95uC for 3 min, followed by 40 cycles of 95uC for 20 s, 62uC for 20s, and 72uC for 20 ...linearity of amplification ...cDNA of different samples (a twofold dilution series with ... See full document

... function in SLE - it could inhibit inflammatory re- sponse by targeting the myeloid differentiation prima- ry response gene 88 (MYD88) and the adapter molecule TAB2 but also promotes inflammatory re- sponse ... See full document

... criteria of the American College of Rheumatology for SLE ...>6, and inactive disease was ...(SLEDAI=10.17±3.7) and 31 patients with no sign of disease activity ...females and ... See full document

... role in defense against infection, particularly those caused by intracellular patho- ...gens. In addition, they are involved in phagocytic removal of cells and cell debris, ... See full document

... distribution and functional activity of Th17 cells in normal BM, PB and LN according to cytokine production, and it is also the first application of an automated ... See full document

... Materials and Methods: Thirty five SLE patients (23 female, 12 male), and 20 age matched controls (10 female, 10 male) participated in this ...from peripheral blood mononuclear ... See full document

... numbers of Th(c)1 cells observed in active SLE in our study and considering the important concept that Th1 or Th2 cells influence the homeostasis of each other [29,30] , ... See full document

... eluted in a 14 μL volume of RNase-free water. In order to quantify the amount of total RNA extracted and verify RNA integrity, samples were analyzed using a 6000 Nano Chip kit, ... See full document

... heterogeneity of human Th17 and how their plasticity shapes the nature of immune cell responses to inflammation and autoimmune diseases, such as systemic lupus ... See full document

... immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) ... See full document

... examination and optic biometry (using Lenstar LS 900 ® , Haag-Streit AG, Koeniz, Switzerland) were ...performed. Blood pressure (BP) was also measured before ...imaging and 10–2 macular auto- mated ... See full document

... Weight and height were measured in pa- tients and controls using an anthropometric ...consisting of girls within the G1-G2 Tanner range, and ii) group 2, consisting of G3-G5 ... See full document

... form of pre- sentation in histoplasmosis. 6, 7 In two studies, stains for fungi per- formed on skin biopsy specimens revealed the organism in 86 to 100 percent of cases of ... See full document

... pathogenesis of SLE is the production of autoanti- bodies that end up acting directly on one or more proteins of the individual, characterizing what is popularly known as an autoimmune ...production ... See full document

... level of cytokine IL-4 observed in patients in our study was contrary to the previous reports of Funauchi et ...(1991) and Shiroiwa et ...study of Wong et al. (2000). Shiroiwa ... See full document

... manifestations and small lesions with high signal intensity in the MRI, which in turn are as- sociated with the presence of APL, would benefit from antiagreggrant therapy based on ...function ... See full document

... ABSTRACT - With the purpose of analyzing the neurological involvement due to systemic lupus erythematosus (SLE), we evaluated 17 female patients who were seen regularly at the hospital [r] ... See full document

... antibodies, lupus anticoagulant, anti-SSA antibodies, anti-SSB antibodies and Complement components 3 and 4 (C3 and ...leukocyturia and erythrocyturia were evaluated through ... See full document