[PDF] Top 20 Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

... Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used tech- nique in ...microbiology. The first step of ... See full document

... to the detection limits from others methods based on ELISA, which usually lie between 10 5 and 10 6 cfu/ml [62, 63], and PNA-FISH, between 10 3 and 10 5 ...[64-66]. The fact that ... See full document

... with the development of peptic ulcer disease, atrophic gastritis, and gastric cancer ...collection of gastric biopsy specimens (11). Noninvasive tests are the most usual methods for routine ... See full document

... The hybridization step in FISH procedure is influenced by several factors including temper- ature, ionic strength and denaturing conditions [ 29 ...Moreover, FISH efficiency also ... See full document

... use the Etest method as it is consid- ered less technically demanding ( 21 , 22 ...However, the Etest and the agar dilution method are more tedious and time-consuming to perform than molecular ... See full document

... Developments on synthetic molecules, such as peptide nucleic acid (PNA), make FISH procedures more robust for microbial ...implementation of PNA-FISH, but ... See full document

... based on the use of a fluorescently labeled nucleic acid probe (generally DNA) that is complementary to a sequence in the rRNA of the microorganism of ... See full document

... founded in December 2010 as a result of the research work developed at the University of Minho and the University of Porto ...company in the biotechnology ... See full document

... for the estimation of the model curvature, and 6 centre points (all factors at coded level 0) repeated to take into account the experimental error (Myers and Montgomery, 1995; Silva et ... See full document

... showing the influence of pH, DS and probe concentration in PNA-FISH for Gram-positive and Gram-negative ...species. The identification of the limiting factor ... See full document

... if the probe Cy3-labeled HP_LNA/2OMe_PS detect the ...against the 16 S rRNA from this bacterial strain, using BLAST software ( ...Then, the hybridization of the Cy3 ... See full document

... days in the former, and the slowest decrease of the viable numbers of lactobacilli after that initial ...period. The lactobacilli profile in the MX cheeses ... See full document

... from the difference of the volumes of mercury intruded in the sample structure at these two extreme pres- ...From the pressure versus intrusion data the equipment ... See full document

... From the territorial variables that could be related to the different performance of the various detection systems, some are illustrated in this ...is the case of ... See full document

... RNA detection both in vitro and in situ, and that the assay can differentiate between SHFV and KRCV-1, which are highly divergent members of the same viral ...determine ... See full document

... sensitivity of NoRISH depends mainly on the quality of mRNA, its retention in the tissue, as well as the accessibility of the target sequences to the ... See full document

... For the past twenty years, several approaches have been devel- oped to try to learn more about the biological way of action of this important class of proteins, but exactly due to their ... See full document

... measurements. In a humid (but unsaturated) atmosphere, the Thorpe sorting is applied to potential temperature ...release of latent heat, making the air parcels ... See full document

... information of Pfeiler [20] and Otake et ...[22]. On the other hand, oikopleurlid larvacean houses and zooplankton fecal pellets were observed in the gut of leptocephali ... See full document

... included in the two self- calibration blocks were used to derive 3D object space coordinates via single photogrammetric intersection using direct determination of the position and orientation ... See full document