[PDF] Top 20 Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

... Immunophenotypic characterization of tumor subspheres For immunophenotypic characterization of tumor subspheres, digital multiparameter flow cytometry was used (FACSARIA, Becton Dickinson, San ... See full document

... abdomen and about one gram of fat tissue was ...pieces of fat transferred into a sterile falcon tube and collagenase was ...the cells were extracted and ...the cells were ... See full document

... properties of dMSCs. One thing should be mentioned is that dMSCs and SKPs could only be isolated simultaneously from the same skin sample when SKP proliferation medium was used during the initial ... See full document

... Isolation of human adipose-derived stem cells (ASCs) Adipose tissue samples were obtained from the subcutaneous abdominal tissue of ten adult female patients, aged 32-49 ... See full document

... work of Cohnheim in 1867 on non-hematopoietic stem cells in the bone marrow followed by Friedenstein and coworkers in the 1960s on the isolation, culture, and osteogenic ... See full document

... mesenchymal stem cells (hUC-MSCs) have recently been recognized as a potential source for cell-based therapy in various preclinical animal models, such as Parkinson’s disease, cerebral ischemia, spinal cord ... See full document

... Tissue Stem Cell Committee", of the International Society for Cellular Therapy, proposed the minimal criteria to define human MSC (Dominici et ...pattern of expression of surface ... See full document

... derive from in vivo experiments in nude ...initiating human cells that are not conducive to growth in a non-permissive mouse microenvironment, as recently suggested by Shmelkov et ...[43]. ... See full document

... Stem cells are undifferentiated cells with a high proliferation ...These cells can be characterized by their in vivo ability to self-renew and to differentiate into specialized cell ... See full document

... under cultivation conditions, and present surface antigen expression patterns including CD29, CD44 (WAGNER et ...CD90 and CD105 (DE MATTOS CARVALHO et ...expression of the hematopoietic ... See full document

... dye-loaded cells were washed and maintained on coverslips for 30 ...stage of an inverted microscope (DM IRE2; Leica Microsystems, Wetzlar, Germany) attached to a confocal laser scanning system ... See full document

... isolate stem cells. The biopsy was cut into thin pieces and treated with trypsin at 4° C overnight (cold trypsin method) to obtain a single-cell ...The cells were seeded at a density of ... See full document

... GENNERO, L.; ROSS, M. A.; SPERBER, K.; DENYSENKO, T.; BERNABEI, P.; CALISTI, G. F.; PAPOTTI, M.; CAPPIA, S.; PAGNI, R.; AIMO, G.; MENGOZZI, G.; CAVALLO, G.; REGUZZI, S.; PESCARMONA, G. P.; PONZETTO, A. Pluripotent ... See full document

... for stem cell research and clinical ...embryonic stem cells (pESCs) and high quality porcine induced pluripotent stem cells ...pluripotent and metabolic features ... See full document

... cultures and stem cell- like growth conditions incorporating the important stem cell aspect in the ...The stem cell-like growth condition seemed suitable for brain slice cultures since the ... See full document

... character of papilla derived ...none of the fibroblast derived m-SKPs expressed ...transcripts of the Oct-4 pluripotency gene have been identified in porcine back skin SKPs [23], murine SKPs [24] ... See full document

... isolated from adult male Wistar EPM rats (weight 250 – 300 g, n = ...tissue from inguinal region was enzymatically dissociated for 30 min at 37 °C by ...atmosphere of 95% O2 and 5% CO2 ... See full document

... immunosuppressive and may allevi- ate GVHD, it is not known whether they will increase the risk of leukemic relapse by abrogating the GVL ...suffering from hematopoietic malignancies. The median ... See full document

... data from our experiment demonstrates compatibility with the results from other studies (Table 1), confirming the requirements proposed by De Schauwer and colleagues (2011) for the appropriate ... See full document

... secretome of human oligodendrocytes (F3.Olig2 cells) generated from human neural stem cells by transduction with the gene encoding the Olig2 transcription ...sequencing ... See full document