[PDF] Top 20 Isolation, culture, characterization and cryopreservation of stem cells derived from amniotic mesenchymal layer and umbilical cord tissue of bovine fetuses

... 2013. Isolation, culture and differen- tiation of mesenchymal stem cells derived from equine amniotic fluid obtained from different gestational ... See full document

... processed and the isolated cells were cultured in DMEM. The cells were subjected to phenotypic characterization and to osteogenic, adipogenic, and condrogenic differentiation to ... See full document

... here and also showing the ability of adipogenic and osteogenic differ- ...ent from those employed in the present study, the cell type is probably the same in view of the similarities ... See full document

... originating from mUC-MSCs were also established using single cell per well dilution in a 96-well plate and microscopically monitoring of growth of each single cell-derived colony was ... See full document

... patterns of MSC from adipose tissue, UCB and ...obtained from different sources might exhibit significant differences in global gene expression patterns that are likely related to their ... See full document

... source of mesenchymal stem cells (MSCs) that had not yet been tested for bovine embryo production by nuclear transfer ...objective of this study was to isolate, characterize ... See full document

... the isolation and culture procedure of mesenchymnal cells of umbilical cord blood without Ficoll-Paque density ...nucleated cells present in the ... See full document

... abdomen and about one gram of fat tissue was ...pieces of fat transferred into a sterile falcon tube and collagenase was ...the cells were extracted and ...the ... See full document

... hematopoietic stem cell (HSC) could repopulate the hematopoietic system of a living recipient, and that when re-isolated from this primary recipient, this cell’s progeny could reconstitute the ... See full document

... tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the ... See full document

... work of Cohnheim in 1867 on non-hematopoietic stem cells in the bone marrow followed by Friedenstein and coworkers in the 1960s on the isolation, culture, and osteogenic ... See full document

... the isolation and expansion of autologous ...use of a more homoge- neous cell population with a proven capacity for differenti- ation into various lineages [5,18,31], by taking MSCs ... See full document

... Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for treatment of a variety of different diseases in human and veterinary ...medicine. ... See full document

... obtained and isolated from bone marrow and adipose tissue as described previously ...subjects, and with the approval of the Ethics Committee of Fundac¸a˜o Oswaldo Cruz, ... See full document

... the isolation of cells rep- resenting stem/progenitor cells of different ...lack of specific MSC markers makes their identification and study more difficult, but ... See full document

... combination of isolation techniques has been used as an attempt to obtain highly pure cell ...majority of protocols begin with a differential plating of the initial cell suspension to ... See full document

... CD73, and CD90 but not CD45, CD34, CD14, CD11b, CD79a, or CD19, or HLA-DR surface ...adipocytes, and chondroblasts in vitro, as MSCs have multiple differentiation ...adipose tissue-derived ... See full document

... Mesenchymal stem cells (MSCs) are present in most of adult tissues, and are therefore attractive for cell ...Because of their high potential for differentiation and ... See full document

... fetal bovine serum (FBS), penicillin (100 mg/mL), streptomycin (100 mg/mL) and fungizone (2 ...entiation of monocytes was induced by a mixture of recombinant GM-CSF (rGM-CSF, CellGenix, ... See full document

... placenta and is already known that it can cause extensive damage to the fetus, since nicotine is clearly neuroteratogenic and impacts the fetal brain at critical stages of development in pregnant ... See full document