[PDF] Top 20 MATERIALS AND METHODS Organism, maintenance and culture condition
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MATERIALS AND METHODS Organism, maintenance and culture condition
... 5.5 and the next lowest 6 ...secreted and exposed affects the ionization state of its amino acids, which dictates the primary and secondary structure of the enzyme, thus controlling its activity ... See full document
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MATERIALS AND METHODS Yeast strain and culture condition
... oligosacharides and oligonucleotides mainly for pharmaceutical and food industries ...human and animal consumption and possesses an arsenal of hydrolytic extracellular hydrolases that enables ... See full document
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MATERIALS AND METHODS Organism
... repression (15). It has been reported that strains of Saccharomyces cerevisiae that were resistant to 2-DG had enhanced fermentation (6). Similarly, strains of the edible mushroom Pleurotus ostreatus that were showed ... See full document
5
MATERIALS AND METHODS Yeast culture and its maintenance
... Statistical optimization of a process or formulation of a fermentation medium can overcome the limitations of classical empirical methods. A successful application of RSM to enhance exoinulinase production from K. ... See full document
9
MATERIALS AND METHODS Bacterial Strains and culture conditions
... (LLO) and Phospholipases (plcA & plcB)(12), all of which facilitate the intracellular growth and spread of the bacterium within a mammalian ...intestine and attribute to the pertinacity of ... See full document
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MATERIALS AND METHODS Microorganism maintenance and culture conditions
... intermediate. This makes laccase the best candidate for the environmentally benign process (12). Laccases are currently seen as highly interesting industrial enzymes because of their wide variety of potential substrates. ... See full document
5
MATERIALS AND METHODS Organism
... the culture medium from ...protein and corn steep liquor on protease production its amounts were also varied in the culture medium from ...incubating culture flasks at 42, 45, 50 and ... See full document
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MATERIALS AND METHODS Isolation and maintenance of microbes
... pathogen and Trichoderma as well as the test fungus alone as a control (C) at 6 days after inoculation ...plate and transferred mycelia mat to conical ...into culture medium of conical flask ... See full document
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MATERIALS AND METHODS Organism
... (Pr1) and trypsin-like (Pr2) cuticle-degrading ...buffered and unbuffered ...dropped and Pr1 and Pr2 activities were detected in high amounts only at a pH of ...Pr1 and Pr2 activities ... See full document
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MATERIALS AND METHODS Organism
... SMI-2 and protease production was observed for 12 hours in liquid medium with 1% trisodium citrate as a carbon source in a 250 mL Erlenmeyer flask ...fast and the formation of protease started from 5h of ... See full document
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MATERIALS AND METHODS Microorganism and culture conditions
... of culture temperature on total fatty acid profile As shown in Table 1 and Figure 3, the major saturated fatty acid found at all cultivation temperatures was palmitic acid (C16:0) and the major ... See full document
14
MATERIALS AND METHODS Organism and growth conditions
... samples and identified as Pseudomonas and Arthrobacter (Strains 3B, 4B, 7B, 21B, 18E and 21D), were tested for the yield of polysaccharides produced during growth in two culture media: one ... See full document
6
MATERIALS AND METHODS Strain and maintenance
... C/N-ratio and absolute docosahexaenoic (DHA)-levels fell in narrow range (5-6 ...rate and/or incomplete glucose utilization occurred. Glucose and FA- fluxes based on fat-free biomass peaked at a C/N ... See full document
14
MATERIALS AND METHODS Microorganisms and culture conditions
... sources and identified to the species level according to recent identification schemes ...of organism suggested possible clinical significance demonstrated the predominance of certain species in different ... See full document
6
MATERIALS AND METHODS Culture Conditions
... intoxication and death of animals and ...rapid and reproducible colorimetric ...light and temperature condition (12/12h light/dark using 30 ...6 and 4 µM) and ... See full document
7
MATERIALS AND METHODS Organism, Growth and Maintenance
... proteases and alpha amylase using a computer controlled laboratory scale ...growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha ... See full document
8
MATERIALS AND METHODS Organism, maintenance and culture conditions
... extracellular and intracellular fructosyltransferases, the present paper reports the results of optimization of constituents in the culture medium and environmental parameters like cultivation time, ... See full document
6
MATERIALS AND METHODS Organism and culture conditions
... metabolized and, therefore, repressive ammonia salts and aminoacids do not accumulate ...medium, and the inhibitory activity occurred Table ...tricholoma and Polyporus tenuiculus cultivation ... See full document
8
MATERIALS AND METHODS Organism and culture conditions
... terrestris and S. cellulophilum, 55ºC for T. Reesei, and 55ºC for Penicillium sp ...75ºC and at 85ºC the crude enzyme still had 60% of the original ...temperature, and at 70ºC only 22% of the ... See full document
7
MATERIALS AND METHODS Microorganism and culture maintenance
... Isomaltulose [O-α-D-glucopiranosyl-(1→6)-β-D-frutofuranose] is a reducing disaccharide commercially obtained by the enzymatic conversion from sucrose catalyzed by a microbial glucosyltransferase as shown in Fig. 1. ... See full document
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