[PDF] Top 20 Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
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Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
... performed using the short read assembling program Trinity (version release-20121005) ...length of overlap to form longer fragments without N, forming ...and using paired- end reads, the software was ... See full document
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Differential Gene Expression (DGE) by RNA sequencing analysis and development of software for integrating different DGE methods
... potential of the software. TheArabidopsis experiment was designed using four biological replicates with two samples for wild type (WT) and two for the mutant NIK T474D lines (Santos et ...mRNA-Seq ... See full document
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Next-generation sequencing-based transcriptome analysis of Helicoverpa armigera Larvae immune-primed with Photorhabdus luminescens TT01.
... development of next-generation sequencing (NGS), also called high-throughput or deep sequencing technology has provided a powerful, highly reproducible and cost-efficient tool for transcriptomic ... See full document
13
De novo transcriptome sequencing of the Octopus vulgaris hemocytes using Illumina RNA-Seq technology: response to the infection by the gastrointestinal parasite Aggregata octopiana.
... milliliter of hemolymph from each octopus was centrifuged at 120006g, 4uC for 5 ...pellet of hemocytes was re-suspended in 1 ml of Trizol reagent (Invitrogen) and stored at 280uC until the ... See full document
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Analysis of the transcriptome of Erigeron breviscapus uncovers putative scutellarin and chlorogenic acids biosynthetic genes and genetic markers.
... Findings: Using Illumina sequencing on GAIIx platform, a total of 64,605,972 raw sequencing reads were generated and assembled into 73,092 non-redundant ...homologues of maize P1 ... See full document
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Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).
... our sequencing results were selected and detected by RT-PCR and qRT-PCR (Table 2) with unigene-specific primers (Table ...the Illumina data, the highest up-regulation of comp66553_c0_seq1 was ... See full document
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De novo assembly and transcriptome analysis of the rubber tree (Hevea brasiliensis) and SNP markers development for rubber biosynthesis pathways.
... source of natural rubber that is native to the Amazon ...properties of natural rubber make it superior to and competitive with synthetic rubber for use in several ...RNA sequencing (RNA-seq) ... See full document
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Transcriptome sequencing and expression analysis of terpenoid biosynthesis genes in Litsea cubeba.
... found using the transcriptome results. An analysis of the expression pattern of 16 genes related to terpenoid biosynthesis showed that there are 12 genes with high expression levels in ... See full document
14
Characterization of the floral transcriptome of Moso bamboo (Phyllostachys edulis) at different flowering developmental stages by transcriptome sequencing and RNA-seq analysis.
... validity of Illumina analysis and assess the expression profiles in terms of specific mRNA abundances, 8 putative genes were selected and detected by qRT-PCR, and to further investigate the ... See full document
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De Novo Transcriptome Assembly of the Chinese Swamp Buffalo by RNA Sequencing and SSR Marker Discovery.
... lives of small farmers and has tremendous economic ...lack of genomic information has hampered research on augmenting marker assisted breeding programs in this ...transcriptomic sequencing of ... See full document
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Nuclear RNA sequencing of the mouse erythroid cell transcriptome.
... to Illumina PE genomic protocol, incorporating improvements suggested in Quail et al 2008, with all reactions scaled according to starting DNA quantity ...[109,110]. Using the Illumina GA-IIx ... See full document
14
Transcriptome analysis of the Asian honey bee Apis cerana cerana.
... samples using the SV total RNA isolation system (Promega, USA) according to the manufacturer’s ...amount of RNA from each stage) as one sample for transcriptome sequencing to obtain as much ... See full document
11
Transcriptome sequencing and De Novo analysis of Youngia japonica using the illumina platform.
... for Illumina Sequencing The mRNA-seq library was constructed using an mRNA-Seq Sample Preparation Kit (Cat# RS-930-1001, Illumina ...cDNA using reverse transcriptase (Invitrogen, ... See full document
10
Transcriptome analysis of androgenic gland for discovery of novel genes from the oriental river prawn, Macrobrachium nipponense, using Illumina Hiseq 2000.
... total of 6 ubiquitin gene families and 8 HSP gene families were found in the ...series of ubiquitin-conjugat- ing enzyme E2 (E2) transcripts are essential for oogenesis and spermatogenesis, as its ... See full document
13
Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux.
... virulence of different ...ml of LB containing ...volume of PBS. Groups of five mice for each strain tested were orally inoculated using a gavage needle with 200 m l of the ... See full document
15
Transcriptome response to elevated atmospheric CO2 concentration in the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)
... abundance of all genes was normalized and calculated by RSEM (Li & Dewey, 2011) and represented by the fragments per kilo base of transcript per million mapped reads (FPKM) value (Trapnell et ... See full document
23
Microbiota present in cystic fibrosis lungs as revealed by whole genome sequencing.
... despite using several DNA extraction methods in parallel to detect as many microbial groups as possible, we cannot exclude biases in the proportions of microbiota members due to varying efficiencies ... See full document
7
Characterization of Liaoning cashmere goat transcriptome: sequencing, de novo assembly, functional annotation and comparative analysis.
... length of 416 ...assembly, using default ...454 sequencing, primers and low quality sequences, using ...the Transcriptome Shotgun Assembly (TSA) database. This Transcriptome ... See full document
11
Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.
... examples of polycistronic RNAs were also reported in some higher eukaryotes but their presence was generally considered ...read sequencing strategy to identify polycistronic transcripts in several mushroom ... See full document
15
Assembly and annotation of the sardine (Sardina pilchardus) transcriptome
... fish of high cultural and economic importance in Portugal and current stock assessment studies report an alarming stock biomass decrease due to overfishing and/or environmental ...management of the sardine ... See full document
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