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Effect of immunization with protoscolices antigens of hydatid cysts on growth of Echinococcus granulosus in dogs

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Effect of immunization with protoscolices antigens of hydatid cysts on growth of

Echinococcus granulosus

in dogs

M. A. Aljawady and M. N. Al-Shahery

Department of Microbiology, College of Veterinary Medicine, University of Mousl, Mosul, Iraq

Abstract

The study was designed to evaluate the immune response of puppies, injected by different protein fractions extracted from protoscolieces, obtained from ovine hydatid cyst. Indirect heamagglutination revealed a remarkable increase in the antibody titers for the immunized groups (A1.5, A3, B1.5, and B3) before and after challenge when compared with the control. Biological variations showed decline in numbers of adult Echinococcus granulosus in the immunized groups when compared with the control. Other variations proved dropping in numbers of the worms within the same immunized groups. Subsequent reductions of cestodes were reported which were 83.8%, 81.3%, 78.2% and 74.6% for the groups A3, A1.5, B3, and B1.5, respectively.

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1. Andersen FL, Ouhelli H, Kachani M. Compendium on cystic echinococcosis in Africa and in Middle Eastern countries with Special Reference to Morocco. Brigham Young University print services, provo, Utah 84604,1997:54-85.

2. Al-Khalidi NW. Investigation of the Immunity of Dogs to

Echinococcus granulosus during the prepatent infection. [dissertation], Ohio State University; 1982:1-35p.

3. Zhang W, Li J, McManus DP. Concepts in Immunology and Diagnosis of Hydatid Disease. Clin Microbiol Rev. 2003;16(1):18-36. 4. Zhang WB, Zhang ZZ, Chi PS. Vaccination of dogs against

Echinococcus granulosus using soluble antigens of protoscolieces. Chin J Vet Sci Technol. 1999;29:21-22.

5. Smyth JD. In vitro culture of Echinococcus spp. Proc. 13th Int Cong Hydat Madrid 1985:84-95p.

6. Smyth JD, Barett NJ. Procedures for testing the viability of Human Hydatid cysts following surgical removal especially after chemotherapy. Trans Roy Soc Trop Med Hyg. 1980;74:649-652. 7. Taherkhani H, Rogan MT. General characterization of laminated layer

of Echinococcus granulosus. Irn J Med Sci. 2000;25:3-4.

. C !

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5AD $%D , 3 ) < 3 H() 5 / "'# =

C

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9. Robyte JF, White BJ. Gel permeation chromatography. II. Organic solvent protein precipitation. in: Biochemical Techniques, Theory and practice. Wadsworth Inc., Belmont, California, USA. 1987;88-268p. 10.Hudson L, Hay FC. Application of gel chromatography. In: practical

immunology, 1st edition, p156. Blackwell Scientific publication Osney

Mead, Oxford, London Edinburgh, U.K. North Balwyn, Victoria, Australia. 1976.

11.Eckert J, Schantz PM, Gasser RB, Torgerson PR, Bessonov AS, Movses-sian SO, Thakur A, Grimm F, Nikogossian MA. Geographic distribution and prevalence, p. 100-142. In: Eckert, J. Gemmell, M. A. Meslin, F.-X. and Pawlowski Z. S. (ed.), WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. World Organisation for Animal Health, Paris, France. 2001.

12.Alton GG. Recent development in vaccination against bovine brucellosis. Aust Vet J. 1978;54:551-557.

13.Dalsgaard K. Adjuvants. Vet Immunol Immunopathol. 1987;17:145-152.

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