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Whole-genome analysis of temporal gene expression during early transdifferentiation of human lung alveolar epithelial type 2 cells in vitro.

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Academic year: 2017

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Figure 1. In vitro culture of adult human alveolar epithelial cells. (A) Human lung AT2 cell morphology changes more when cultured on rat- rat-tail collagen-coated dishes than on Matrigel-coated dishes
Figure 2. Candidate genes’ expression patterns. Genes expressed differentially in hAT2 cells on collagen compared to Matrigel with P,0.01 were analyzed based on expression dynamics and sorted into one of five expression patterns
Figure 3. Validation of a subset of candidate genes by qRT-PCR. RNA was harvested from hAT2 cells cultured on collagen or Matrigel at 12 hours and 1, 2, and 3 days after seeding, n = 2
Figure 5. Sub-cellular localization of PTRF/CAVIN-1 changes from nuclear to cytoplasmic in transitioning hAT2 cells

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