A549 Cells
TGF-β1 downregulates COX-2 expression leading to decrease of PGE2 production in human lung cancer A549 cells, which is involved in fibrotic response to TGF-β1.
... stability, cells were pretreated with the transcriptional inhibitor actinomycin D before TGF-β1 ...Control cells treated with actinomycin D alone showed a significant decrease in COX-2 ...control ...
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Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells.
... Despite eliciting eIF2α phosphorylation, gefitinib and erlotinib did not activate PERK, which is one of the sensor molecules in ER stress [35, 36]. Additionally, these two drugs did not change the mRNA expressions of ...
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Viral retasking of hBre1/RNF20 to recruit hPaf1 for transcriptional activation.
... To determine whether hPaf1 is recruited to the HAdV genome, we performed ChIP analysis. A549 cells were infected with WT HAdV and a series of HAdV containing deletions in E1A at an MOI of 5. ChIP was then ...
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Gil Benard 2,3, Maria José Soares Mendes Giannini
... epithelial cells has been studied, with particular emphasis on the induction of ...human A549 cells, we infected these cells with ...human A549 cells was induced by ...
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Blockade of Hedgehog Signaling Synergistically Increases Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Cancer Cell Lines.
... lines A549 and H1975were treated with 40 μM SANT-1 alone, 20 nM gefitinib alone, or a combination of SANT-1 and ...lines A549 and H1975 with increasing concentrations of SANT-1 alone, gefitinib alone, and ...
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Crude alkaloid extract of Rhazya stricta inhibits cell growth and sensitizes
... line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of ...of A549 cell growth correlated with the induc- tion of apoptosis ...
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Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
... on A549 cells reveals the same ...macrophage-like cells (differentiated THP-1 cells). These cells are apparently less sensitive than the other two cells lines, as more amenable ...
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MiRNA 17 family regulates cisplatin-resistant and metastasis by targeting TGFbetaR2 in NSCLC.
... MicroRNAs (miRNAs) have been proven to play crucial roles in cancer, including tumor chemotherapy resistance and metastasis of non-small-cell lung cancer (NSCLC). TGFb signal pathway abnormality is widely found in cancer ...
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Overexpression of the lung cancer-prognostic miR-146b microRNAs has a minimal and negative effect on the malignant phenotype of A549 lung cancer cells.
... the cells was assessed in two different types of assays: an in vitro wound healing assay to test the ability of a cell monolayer to close a gap created by a scratch, and a transwell assay to test the movement of ...
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Paracoccidioides brasiliensis induces secretion of IL-6 and IL-8 by lung epithelial cells. Modulation of host cytokine levels by fungal proteases
... epithelial A549 cells, which is dependent on activation of p38 MAPK and ERK ...in A549-live ...epithelial cells and degradation of these cytokines by fungal ...
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Aqueous Extract of Huang-lian Induces Apoptosis in Lung Cancer Cells via P53- Mediated Mitochondrial Apoptosis
... Apoptosis induced by some anticancer agents constitutes one aspect of their treatment effect. Two major pathways involved in the process have been investigated in great depth (Gustafsson and Gottlieb 2007). As p53 ...
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Protective roles of pulmonary rehabilitation mixture in experimental pulmonary fibrosis in vitro and in vivo
... AT I covers more than 90% of the alveolar surface area of the peripheral lung, and interfaces with pulmonary capil- laries, providing a surface readily permeable to gases. In- creased AT I apoptosis leads to ineffective ...
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Mutations in polymerase genes enhanced the virulence of 2009 pandemic H1N1 influenza virus in mice.
... human A549 cells and porcine PK15 cells in vitro, without significant effect on viral propagation in murine LA-4 cells and pathogenicity in mice; however, it did enhance the lung virus ...in ...
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Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells.
... this, A549 cells were fixed with acetone and stained for 53BP1 and c-H2AX after MIR ...unexposed cells, but formed numerous distinguished subnuclear foci in response to ...
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Clonagem e caracterização da proteína 14-3-3 de Paracoccidioides brasiliensis durante sua interação com células epiteliais
... with A549 cells, we observed an apparent increase in the levels of the 14-3-3 protein the cell wall of the fungus, suggesting that this protein may be involved in host-parasite ...of A549 ...
133
The splicing factor proline-glutamine rich (SFPQ/PSF) is involved in influenza virus transcription.
... of cells infected with VIC influenza ...infected cells we carried out co-immunoprecipitation experiments. Cultures of A549 cells were infected at high moi with the VIC strain of influenza ...
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Let-7 Sensitizes KRAS Mutant Tumor Cells to Chemotherapy.
... Both A549 and Panc-1 cell lines are well characterized for their high motility [24,25], which is reflective of the metastatic nature of NSCLC and ...tumor cells. As shown in Fig 5A, A549 and Panc-1 ...
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Inhalable antitubercular therapy mediated by locust bean gum microparticles
... epithelial cells (A549 cells) and macrophages (THP-1 cells) revealed a toxic effect from rifabutin-loaded microparticles at the highest concentrations, but we may consider that these were very ...
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Fibroblast α11β1 integrin regulates tensional homeostasis in fibroblast/A549 carcinoma heterospheroids.
... carcinoma cells and fibroblasts, involving a central role for avb6 on carcinoma cells in activating TGF-b and initiating activation of fibroblasts ...study A549 cells were found to express low ...
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26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro.
... from A549 and H1299 cells differ in their tumorigenic potential compared to cells grown in a monolayer, we injected 10,000 cells subcutaneously from each of these populations into the flanks ...
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