Comet Assay
Bovine Papillomavirus Clastogenic Effect Analyzed in Comet Assay
... animals. Comet assay was performed according to alkaline ...he comet assay is considered an eicient tool for assessment of damage in the host chromatin due to viral action, speciically ...
8
Optimization of Neutral Comet Assay for studying DNA double-strand breaks in pea and wheat
... of Comet assay protocols have been developed for different type of studies in ...the Comet assay are available in plants: A/A (alkaline lysis and electrophoresis), N/N (neutral lysis and ...
7
Comet assay comparison of different Corbicula fluminea (Mollusca) tissues for the detection of genotoxicity
... Mitchelmore et al. (1998) reported that 20% of M. edulis digestive gland cells can be expected to exhibit low-level DNA damage (measurable by the comet assay) caused by cell-breakage during isolation and ...
5
An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents
... (SCG) assay, a new procedure for evaluating DNA lesions (single strand breaks and alkali-labile sites), involves application of an electrical current to cells, which results in the trans- port of DNA fragments out ...
6
DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay
... the comet assay II automatic digital analysis system (Perceptive Instruments ...(comet assay endpoint treated - comet assay endpoint control)/OTA concentration ...
6
Assessment of the DNA Damage in Human Sperm and Lymphocytes Exposed to the Carcinogen Food Contaminant Furan with Comet Assay
... The cells were checked for their viability before the start and after the completion of the experiment using trypan blue dye (Pool-Zobel et al. 1992). Preparation of the slides for the comet assay and ...
8
The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay
... alkaline comet assay method described by Singh et ...of comet assay involved at least three hours of cell lysis by detergent at a high salt concentration (1% Triton X-100, 10% DMSO, Stock ...
8
Estimates of DNA damage by the comet assay in the direct-developing frog Eleutherodactylus johnstonei (Anura, Eleutherodactylidae)
... the Comet assay to assess genetic damage in the direct-developing frog Eleutherodactylus ...diffusion assay was used to evaluate the effectiveness of alkaline, enzymatic and alkaline/enzymatic ...
8
Evaluation of Sinos River water genotoxicity using the comet assay in fish
... the comet assay to detect genotoxicity due to multiple sources of pollution in the peripheral blood of a native fish species (Hyphessobrycon ...the comet assay demonstrated no significant ...
6
Chromatin in fractal globule state: evidence from comet assay
... In our previous works we have shown that single cell gel electrophoresis (or the comet assay) is an ap- proach which may be applied to study the DNA loop- ing [20, 21]. The method is based on the analysis ...
7
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
... The Comet Assay, or single cell gel electrophoresis, is a sensitive genotoxicological method for assessing DNA damage in single cells, allowing for the quantification of DNA breaks and alkaline labile ...
4
Comet Assay as a technique to evaluate DNA damage in sickle cell anemia patients
... disease, due to constant oxidative stress and membrane lesions, this assay can be useful to detect DNA lesion intensity and medication response. Figure 1A illustrates cell nuclei from a 31-year- old sickle cell ...
1
Wood smoke exposure of Portuguese wildland firefighters: DNA and oxidative damage evaluation
... The comet assay or single gel electrophor- esis is a valuable effect biomarker that quantifies DNA damage in populations exposed to various types of agents (Collins et ...
10
Alterações genéticas relacionadas à obesidade: danos no DNA, perfil de expressão e polimorfismos gênicos
... mg/ml bovine serum albumin and 0.5 mM EDTA at pH 8). Slides were incubated at 37ºC for 30 min in a moist and dry chamber, with 100 µl of fpg and endo III (1:1000) enzymes which recognize oxidised purine and pyrimidine, ...
90
Evaluation of prevention of DNA damage and induction of DNA repair by natural compounds
... in somatic or germinative cells. Procedures which help in hazard prediction and risk assessment are important to assess the genotoxic potential of chemicals before their release into the environment or commercial use as ...
39
Estudo do potencial genotóxico da Gutiferona A em diferentes células de camundongos in vitro
... The comet assay (SCGE) was carried out by the method described by Speit and Hartmann [10] and reviewed by Burlinson et al. [11]. Peripheral blood samples from the tail vein were obtained from six Swiss mice ...
67
Characterization of cell integrity of Planctomycetes species : signaling and DNA protection
... the Comet Assay is not an effective method for UVC mediated DNA damage detection in ...the assay were modified taking into account that UVC damage is different from the damage caused by hydrogen ...
99
Haematological and genotoxic profile study of workers exposed to medical waste
... comet assay. Despite some DNA damage being repaired, it is necessary the permanent biomonitoring for genotoxicity in MW, with the use of biomarkers for prevention of future DNA lesions, which may induce ...
9
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, southern Brazil
... The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos ...
8
Citogenotoxic response of juvenile cobia Rachycentron canadum (Linnaeus, 1766) reared in two different systems
... using comet assay and micronucleus and other nuclear abnormalities ...The comet assay and the nuclear abnormalities assay detected higher DNA damage and higher nuclear ab- normalities ...
10