Fluorescent protein
Study of the antimalarial properties of hydroxyethylamine derivatives using green fluorescent protein transformed Plasmodium berghei
... A rapid decrease in parasitaemia remains the major goal for new antimalarial drugs and thus, in vivo models must provide precise results concerning parasitaemia modulation. Hydroxyethylamine comprise an important group ...
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An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.
... novel fluorescent protein constructs were subcloned from the pQE9 vector into the pEGFP-C1 using NheI and HindIII restriction ...associated protein 4, nucleotides 1918–3135); (P) ...
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Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene
... Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements ...
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Fluorescent-protein stabilization and high-resolution imaging of cleared, intact mouse brains.
... S1 Fig. Analysis of EGFP fluorescence during dehydration, clearing and rehydration in vitro using different conditions. (A) Fluorescence of EGFP expressed in E. coli after dehydra- tion with different alcohols followed ...
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Induction of cell stress in neurons from transgenic mice expressing yellow fluorescent protein: implications for neurodegeneration research.
... of fluorescent proteins such as GFP, YFP and CFP (collectively termed XFPs) in neurons, under the control of the neuronal-specific thy1 gene, has revolutionised modern neuroscience research, facilitating ...
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A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes
... The inspection of fermentations for the presence of contaminant yeasts could be improved if morphological marker genes could be introduced in industrial yeasts preserving their technological properties. For this purpose ...
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Application of Fluorescent Protein Expressing Strains to Evaluation of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
... developed fluorescent protein (FP) expressing Myco- bacterium bovis BCG strains for in vivo imaging, which can be used to track bacterial loca- tion, and to quantify bacterial load in live ...their ...
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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
... green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from ...Green fluorescent protein (GFP) was successfully expressed in ...
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Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts.
... limited by the penetration depths of visible light in the body [10]. However, proteins with excitation and emission maxima within a near-infrared window from ,650 nm to 900 nm can be used for in vivo imaging as they have ...
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Functional fluorescent protein insertions in herpes simplex virus gB report on gB conformation before and after execution of membrane fusion.
... with fluorescent protein DNA fragments flanked by AvrII sites at both ...ends. Fluorescent protein DNA fragments with sticky ends were generated by PCR amplification and AvrII digestion from ...
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Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation
... quence of receptor desensitization. Receptor desensitization plays an important physiologi- cal role by preventing overstimulation of GPCR signaling cascades which in the case of constitutively active mutant receptors ...
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Selective chemokine receptor usage by central nervous system myeloid cells in CCR2-red fluorescent protein knock-in mice.
... Circulating blood monocytes in the Ccr2 RFP/+ mice were uniformly positive for RFP by FACS, and approximately 80% expressed cell- surface CCR2, whereas CCR2 surface-negative monocytes were predominantly Ly6C lo , as ...
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Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
... Total RNA extraction and subsequent cDNA synthesis were performed using the TRIzol Plus RNA Purification Kit (Life Technologies, Carlsbad, CA, USA) and the SuperScript III First-Strand Synthesis System (Life ...
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mKikGR, a monomeric photoswitchable fluorescent protein.
... The significantly improved photobleaching stability of mKikGR compared to other monomeric photoactivatable proteins suggests that use of mKikGR in localization microscopy could result in a further improvement of ...
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ARTIGOS GFP-fluorescent protein on the study of blister spot in coffee plants
... Epifluorescence micrograph showing the colonization and reproduction of Colletotrichum gloeosporioides (40x magnification). a) Hypocotyls at 15 days after inoculation with transformed[r] ...
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Construção de linhagem recombinante de Enterococcus gallinarum para expressão da proteína Enhanced Green Fluorescent Protein (EGFP)
... Os oligonucleotídeos usados para as reações foram o Ega-P-βeta 1 e 2 para amplificação do fragmento de 1000 pares de bases, correspondente à região promotora P-βeta, e VanC1rt_F e [r] ...
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A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
... We used the pBac [3xp3-EGFPafm] (Horn and Wimmer, 2000) plasmid, containing the piggyBac trans- poson inverted terminal repeats (ITRs) and the GFP gene marker under the control of an artificial promoter containing three ...
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Welfare assessment in transgenic pigs expressing green fluorescent protein (GFP)
... Around 119 days of age 39 animals (19 wt, 20 GFP) were individually tested regarding general activity in and their reaction to an unfamiliar environment (Open Field tests – OF1, OF2), [r] ...
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Further insights into metal-DOM interaction: consideration of both fluorescent and non-fluorescent substances.
... contained fluorescent protein-like substances, while not only protein-like but also humic-like substances were observed in ...the protein-like substances in all EPS fractions but the ...
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Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis.
... the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of ...peptide- fluorescent bacteria ...
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