Green fluorescent protein
Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene
... Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting ...
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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
... The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from ...goat. Green fluorescent protein (GFP) was successfully expressed in ...
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Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation
... G protein-coupled receptor (GPCR) activation is followed rapidly by adaptive changes that serve to diminish the responsiveness of a cell to further ...messenger-dependent protein kinases and ...
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Study of the antimalarial properties of hydroxyethylamine derivatives using green fluorescent protein transformed Plasmodium berghei
... A rapid decrease in parasitaemia remains the major goal for new antimalarial drugs and thus, in vivo models must provide precise results concerning parasitaemia modulation. Hydroxyethylamine comprise an important group ...
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Proliferação Celular em Gestações Naturais e de Conceptos Bovinos Transgênicos Clonados, que Expressam Proteína Fluorescente Verde / Cell proliferation in pregnancies natural and transgenic conceptos cattle cloned, expressing green fluorescent protein
... clonados transgênicos, 60 dias de gestação. a) A imunorreatividade foi detectada principalmente no epitélio fetal, e, como nos animais de gestação natural, observamos também a marcação [r] ...
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Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
... subsequently prepared from cultures derived from single colonies that demonstrated green fluorescence when grown in GN media. Quantitative RT-PCR, flow cytometry and quantitative fluorescence analysis was ...
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Fluorescent-protein stabilization and high-resolution imaging of cleared, intact mouse brains.
... hanced Green Fluorescent Protein): most protocols that clear tissue effectively also result in the loss of XFP fluorescence, either immediately [4,7] or within hours or days ...
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Cell type-specific neuroprotective activity of untranslocated prion protein.
... PrP-enhanced green fluorescent protein (PrP-EGFP) fusion molecule [33], and induced robust synthesis of untranslocated PrP-EGFP by treating the cells with an inhibitor of PrP translocation (see ...
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Tackling Glaucoma from within the Brain: An Unfortunate Interplay of BDNF and TrkB.
... enhanced green fluorescent protein; DAPI, 4’,6-diamidino-2-phenylindole; SC, superior colliculus; rAAV2/1, recombinant adeno-associated viral vector serotype 2/1; CMV, cytomegalovirus promoter; IHC, ...
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Contribution of endothelial injury and inflammation in early phase to vein graft failure: the causal factors impact on the development of intimal hyperplasia in murine models.
... Methods and Results: Several microscopic techniques were used to study endothelium denudation and regeneration and leukocyte recruitment on endothelium. Scanning electron microscopy demonstrated denudation of vein graft ...
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Rapid induction of GFP expression by the nitrate reductase promoter in the diatom Phaeodactylum tricornutum
... dependent expression of the green fluorescent protein (GFP) reporter under control of the nitrate reductase promoter in independently genetically transformed P. tricornutum cell lines following ...
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Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis.
... the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of ...peptide- fluorescent ...
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Identificação de componentes da via de sinalização mediada pela proteína NIK, um receptor que interage com a proteína NSP de geminivírus
... fluorescência emitida pela proteína YFP (yellow fluorescent protein) e GFP (green fluorescent protein) foi monitorada por meio de microscopia confocal, em células epidérmicas de tabaco[r] ...
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PROTEíNAS FLUORESCENTES: VANTAGENS NO ESTUDO DOS MECANISMOS qUE MANTÊM A ESTABILIDADE GENÉTICA
... A necessidade de aprofundar o conhecimento relativamente aos mecanismos de desenvolvimento de um organismo, evidenciou a importância de recorrer à observação da distribuição subcelular e dinâmica de proteínas (morin et ...
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Retention of a recombinant GFP protein expressed by the yellow fever 17D virus in the ENS1 intergenic region in the endoplasmic reticulum
... E protein and precursor membrane protein, are mainly associated with the endoplasmic reticulum (ER) through two transmembrane (TM) domains that are exposed to the luminal face of this ...reporter ...
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Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
... enhanced green fluorescent protein (EGFP) reporter into immunodeficient mice bearing 786-O renal cell carcinoma subcutaneous (SC) xenografts and kidney orthotopic (KO) ...EGFP protein levels ...
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Correlating intravital multi-photon microscopy to 3D electron microscopy of invading tumor cells using anatomical reference points.
... ). Scale bars: 100 mm. D-F. The landmark-registration procedure is performed in Amira, using 3D models of collagen fibers as a reference. The selected landmarks (colored ‘targets’) indicate corresponding points in both ...
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Intramolecular ex vivo Fluorescence Resonance Energy Transfer (FRET) of Dihydropyridine Receptor (DHPR) β1a Subunit Reveals Conformational Change Induced by RYR1 in Mouse Skeletal Myotubes.
... revealing interlinked SH3 and GK domains representative of members of the MAGUK super- family of adapter proteins [29–31]. A less ordered domain in between the SH3 and GK domains composed of the variable central domain ...
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Imaging of nitric oxide in nitrergic neuromuscular neurotransmission in the gut.
... NO fluorescent signals from the ...NO fluorescent image and the image in the transmission mode. Note the green fluorescent varicosities seen along axons running in the long axis of smooth ...
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ARTIGOS GFP-fluorescent protein on the study of blister spot in coffee plants
... The predominant species in Brazil is C. gloeosporioides, which is associated with a complex of symptoms that include: anthracnose in fruits, tip blight and blister spot. Symptoms related to blister spot are characterized ...
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