High resolution melting analysis
Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.
... ‘high resolution melting analysis or high-resolution fluorescent melting curve analyses or High-resolution amplicon melting analysis’, ‘HRM or ...
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High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.
... amplicon melting profiles in order to explore polymorphic regions by HRM ...discriminatory melting temperature (Tm) values for Brazilian and Eurasian/African Leishmania ...
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Reliable discrimination of 10 ungulate species using high resolution melting analysis of faecal DNA.
... HRM-PCR analysis used here has six key advantages over other ...run analysis in a closed-tube performance enables high-throughput screening (approximately three times faster compared to Sanger ...
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Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis
... HRM analysis, the PCR products of the 11 Lavandula specimens amplified with the Lav1-F/Lav1-R primers were sequenced in opposite directions, obtaining electropherograms with high resolution and a ...
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High resolution melting analysis as a new tool to authenticate plant food supplements: the case of artichoke (Cynara Scolymus)
... to high resolution melting (HRM) analysis to discriminate ...the analysis of herbal mixtures and PFS labelled as containing artichoke as ...HRM analysis showed that the tested ...
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Combined mutation and rearrangement screening by quantitative PCR high-resolution melting: is it relevant for hereditary recurrent Fever genes?
... denaturing high performance chromatography, HRM, High Resolution Melting analysis) that only allow for the identification of micro- ...quantitative analysis) combined with HRM ...
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Unraveling Host-Vector-Arbovirus Interactions by Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis in a Kenyan Wildlife-Livestock Interface.
... bloodmeal analysis rely on time consuming and expensive sequencing [4– ...on high resolution melting analysis (HRM) of cytochrome b (cyt b) Polymerase Chain Reaction (PCR) products to ...
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Methylation-sensitive high resolution melting (MS-HRM) analysis of SOCS1, P16 and TIMP2 genes in LPS-stimulated human gingival fibroblastos = Análise específica de metilação por melting de alta resolução (MS-HRM) dos genes SOCS1, P16 e TIMP2 em fibroblastos gengivais humanos estimulados por LPS
... se o LPS pode alterar o estado de metilação desses genes. Material e Métodos: As células de fibroblastos gengivais humanos imortalizadas foram cultivadas em meio suplementado com LPS de P. gingivalis ou com o veículo do ...
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High resolution melting : improvements in the genetic diagnosis of hypertrophic cardiomyopathy in a Portuguese cohort
... Peripheral blood was collected from 80 Portuguese unrelated patients and from 100 healthy subjects (con- trols without any cardiovascular disease). With the exception of control individuals, all index patients pre- ...
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Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting
... Rifampicin is considered a good marker for multi- drug resistance. This drug binds to the β-subunit of RNA polymerase encoded by rpoB and inhibits DNA-directed RNA synthesis. Mutations in the rpoB gene are associ- ated ...
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Genetic diagnosis of hypertrophic cardiomyopathy using mass spectrometry DNA arrays and high resolution melting
... a high-resolution melting (HRM) technique in a real-time PCR thermal cycler (LightCycler 480, Roche ...amplification, melting and fluorescence detection conditions were as indicated by the ...
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Detection of somatic mutations by high-resolution DNA melting (HRM) analysis in multiple cancers.
... The sensitivity and specificity for mutation/variant screening were 97% and 87% respectively when compared to bi-directional sequencing, with a false negative rate of 3%. The overall accuracy of the test was 89% (Table ...
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DNA mini-barcodes coupled to high resolution melting (hrm) analysis for the botanical authentication of rosemary honey
... attain high market values, thus being prone to fraudulent ...microscopic analysis are still being used, but they are time‐consuming and greatly dependent on the experience/skill of trained ...to high ...
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UPDATING MAPS USING HIGH RESOLUTION SATELLITE IMAGERY
... The usage of more sophisticated and advanced remote sensing technology solutions to provide reliable and inexpensive information that can be employed for map updating. Improved satellite-based remote sensing instruments ...
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Elemental analysis of aerosol organic nitrates with electron ionization high-resolution mass spectrometry
... Aiken, A. C., Salcedo, D., Cubison, M. J., Huffman, J. A., DeCarlo, P. F., Ulbrich, I. M., Docherty, K. S., Sueper, D., Kimmel, J. R., Worsnop, D. R., Trimborn, A., Northway, M., Stone, E. A., Schauer, J. J., Volkamer, ...
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Elemental analysis of aerosol organic nitrates with electron ionization high-resolution mass spectrometry
... Recently, the high resolution version of the AMS (HR- ToF-MS) has been used to determine elemental composi- tion of organic aerosol (Aiken et al., 2007, 2008). While the AMS is to first order equally ...
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A high-resolution bedrock map for the Antarctic Peninsula
... A number of factors that were not included in the uncertainty assessment (Sect. 4) as their effect on calculated ice thickness is difficult to quantify, hamper the reliability of our high-resolution bedrock ...
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Iterative Single-Image Digital Super-Resolution Using Partial High-Resolution Data
... extracting high-resolution data from low-resolution images is one of the most important digital processing applications in recent years, attracting much ...the resolution of an image when a ...
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Quantitative chemical analysis for the standardization of copaiba oil by high resolution gas chromatograpy.
... CHEMICAL ANALYSIS FOR THE STANDARDIZATION OF COPAIBA OIL BY HIGH RESOLUTION GAS ...for analysis of methylated copaiba oils, using trans-(-)-caryophyllene or methyl copalate as external ...
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Deteção de mutações nos genes JAK2 e MPL por high resolution melting
... Atualmente, estão descritas uma variedade de técnicas moleculares altamente sensíveis para a deteção destas mutações, em particular para a mutação V617F. Estas técnicas incluem PCR alelo específico (AS-PCR) ou ARMS ...
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