LIVING CELLS
A flow cytometry-based FRET assay to identify and analyse protein-protein interactions in living cells.
... interactions with cellular factors [21]. Lentiviral Nef and Vpu proteins are important for efficient viral replication and AIDS progression in vivo [15]. While it has been established that Nef and Vpu manipulate the ...
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Carotenoid distribution in living cells of Haematococcus pluvialis (Chlorophyceae).
... in living H. pluvialis cells using resonance-enhanced confocal Raman microscopy and multivariate curve resolution (MCR) ...cyanobacterial cells [28] and here, we make an important extension of the ...
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Measurement of separase proteolytic activity in single living cells by a fluorogenic flow cytometry assay.
... hematopoietic cells the flow cytometric Separase assay allows generation of Separ- ase activity profiles that tell about the number of Separase positive cells within a sample ...i.e. cells that ...
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An improved model for nucleation-limited ice formation in living cells during freezing.
... in living cells is a lethal event during freezing and its characterization is important to the development of optimal protocols for not only cryopreservation but also cryotherapy ...in cells ...
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Visualization and measurement of ATP levels in living cells replicating hepatitis C virus genome RNA.
... in cells replicating SGR-ATeam, an increase in ATP concentration was observed both at putative replication sites and in the cytoplasm of ...SGR-AT1.03-replicating cells in the presence of PSI-6130 for 10 ...
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Developing new molecular tools to study and visualize the intermediate filament GFAP in living cells
... give cells the elastic properties that allow them to resist deformation and maintain their ...in living cells, and that can easily be implemented in the study of other ...
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Development of a new cellular model for the visualization and study of GFAP oligomerization in living cells
... Figure 6 - Bimolecular fluorescence complementation (BiFC) assays for the visualization of protein-protein interactions in living cells - The proteins of interest are fused to two non-fluorescent halves of ...
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Spectroscopic investigation of local mechanical impedance of living cells.
... in living cells ...the cells is a key observable for diseases diagnostics [6] and cell signalling ...PC12 living cells employing atomic force microscopy ...
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Leishmania (L.) amazonensis peptidase activities inside the living cells and in their lysates
... In this study we investigated the peptidase activity in Leishmania (L.) amazonensis live amastigote by con- focal microscopy using peptidyl-MCA as substrates, the hydrolysis of which releases the MCA fluorophore inside ...
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A novel 3D fibril force assay implicates src in tumor cell force generation in collagen networks.
... New insight into the biomechanics of cancer cell motility in 3D extracellular matrix (ECM) environments would significantly enhance our understanding of aggressive cancers and help identify new targets for intervention. ...
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The percentage of living bacterial cells related to organic carbon release from senescent oceanic phytoplankton
... death cells (Agustí and Sánchez, ...of cells (Agustí and Sánchez, 2002; Agustí, ...stain cells after the CDA, Darzynkiewicz et ...resuspending cells in culture media until the complete ...
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CELL DEATH DIFFERENTIATION IN BLACK HEADED RAMS SPERMATOZOA, USING FLUORESCENT LABELED ANNEXIN V
... sperm cells. When this non-fl uorescent compound enters living cells, esterases present hydrolize it, producing the fl uorescent compound 6-carboxyfl ourescein ...The cells with PS translocation ...
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Enhanced beetle luciferase for high-resolution bioluminescence imaging.
... in living cells to these physicochemical properties ...in living cells contribute more to its much brighter signal in cells than do its physicochemical ...
11
TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES-AMAZING TOOL FOR GENOME EDITING
... Newly developed engineered nuclease Transcription Activator-Like Effector Nucleases (TALENs) can efficiently modify any sequence of interest in living cells or organisms. It can make replacement and gene- ...
4
Evaluation of the cytotoxicity of transition metal complexes: DNA cleavage of copper complexes in cells
... of living bacterial cells by concerned chemical compound, (3) purification of pDNA from exposed bacterial cells, and (4) the AGE of pDNA to check the extent of ...
78
Insights into human carboxylesterase 2 stability and activity in vivo and in vitro
... A higher CES2 than CES1 gene expression has been described for HT-29 cells (36). However, activities from each specific CES were not shown, only being described as “very low total CES activity ”. The present ...
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Live Cell Imaging of Butterfly Pupal and Larval Wings In Vivo.
... epithelial cells at these stages is thus critical to understand how wing structures, including color patterns, are ...epithelial cells in the late larval and early pupal stages 1 hour ...epithelial ...
21
MATERIALS AND METHODS Venom and Reagents
... endothelial cells in living microvessels using intravital microscopy, which showed that it induced a dose-dependent increase in rolling and adherence of leukocytes, acting directly on endothelial ...
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Rev. Saúde Pública vol.45 número4
... “So then, adults come in, don’t they? I think that they should all live together in one place. There should be a place to receive them, you know? For them to have possibilities, you know? And to have someone in charge, ...
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