RNA extraction
An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR
... Plant RNA Reagent) was added to the recovered upper phase, and the initial extraction procedures were ...total RNA extraction from leaves and roots was performed using the PureLink ® ...
9
Improvement in RNA Extraction from S. cerevisie by Opti- mization in the Autolysis and NH3
... the RNA extraction, since it achieved the maximum extrac- tion of ...the RNA extraction on a pilot scale, includ- ing other studies such as precipitation by pH in isoeletric point, and ethanol ...
12
COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
... the advances in passion fruit culture were obtained by genetic enhancement (MelettI, 2011). then, the best method for RnA extraction in P. edulis must be determined so that analyses such as complementary ...
7
RNA extraction of granulosa layer of F1 follicle
... Th e f o llo w in g p rim er seq u en ces f o r t h e PCR r eact io n s w er e in clu d ed : iNOS p r im er s, sen se: 5 ’ -A GGCCA A A CA TCCTGGA GGTC-3 ’ , an t isen se: 5 ’ -TCA TA GA GA CGCTGCTGCCA G-3 ’ an d β -act ...
7
Magnetic bead technology for viral RNA extraction from serum in blood bank screening
... particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) ...viral RNA was performed ...
6
DNA and RNA extraction and analysis: The
... of RNA integrity (Fig. 6 and 7). There is evidence that RNA degradation is caused by RNases, and increased enzymatic activity has been associated with programmed cell death (Panavas et ...remaining ...
12
RNA extraction, RT and Real-Time PCR (qPCR)
... Samples were collected from 1,022 asymptomatic domestic and wild birds from the Brazilian coast and the Amazon region using tracheal/cloacal swabs and tested by RT-qPCR.. The[r] ...
7
PCR-TTGE analysis of 16S rRNA from rainbow trout (Oncorhynchus mykiss) gut microbiota reveals host-specific communities of active bacteria.
... DNA extraction (D) and RNA extraction (R) from different individuals (I) from Family F1, which were fed either the control diet D1 (where 100% of the protein in the diet was provided by fish meal and ...
10
RNA-SEQ applied to the peacock blenny Salaria pavo: unveiling the gene networks and signalling pathways behind phenotypic plasticity in a littoral fish
... July at Culatra island) using snorkelling or SCUBA, contingent on tide conditions, during mid- morning when the tide was high. All individuals were caught in their spawning territories, after verifying through ...
308
Comparison of Four Extraction Methods to Detect Hepatitis A Virus RNA in Serum and Stool Samples
... of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as ...different extraction ...
7
“Análise da viabilidade e expressão de genes de virulência de Streptococcus mutans em lesões dentinárias de crianças com cárie precoce da infância”
... total RNA extraction and purification to conduct studies on bacterial gene expression from in vivo dentine caries lesions (Chapter 01); (b) to identify and quantify the metabolically active SM and analyze ...
74
Mechanical stress is a pro-inflammatory stimulus in the gut: in vitro, in vivo and ex vivo evidence.
... Furthermore, our in vivo study in animal model of lumen distention confirmed that mechanical stress is a potent stimulus to induce production of pro-inflammatory mediators. Interestingly, both our in vitro and in vivo ...
9
Previsão de Produção de Energia Eléctrica através de Fontes de Energia Renováveis
... A RNA constituída apenas por uma camada intermédia, representada na figura 3.8, é uma estrutura simples mas que poderá dar bons resultados. Neste caso, todas as 32 entradas irão dar entrada na mesma camada ...
79
Coronavirus em aves: detecção, caracterização molecular e filogenética e inoculação...
... do RNA viral sem a existência de partículas virais infecciosas disseminando, por exemplo, células fagocitárias; cabe aqui também a sugestão da diferença entre fragmentos testados para PCR e histopatologia em um ...
100
TECNOLOGIA ENZIMÁTICA PARA MELHORAR A EXTRAÇÃO DO ÓLEO DA POLPA DE Caryocar brasiliense (PEQUI)
... oil extraction; in the second, a simple process was carried out by combining sun-drying pulp and ...Aqueous extraction has presented lower efficiency and higher oxidation of unsaturated fatty ...
7
Braz. J. Chem. Eng. vol.29 número2
... Soxhlet extraction, the use of different organic solvents showed that they change the yield of the oil extraction process as well its fatty acid composition, giving statistically different results (P< ...
12
Rev. bras. farmacogn. vol.23 número3
... Serum Albumine (BSA) solution 1 mg/mL in 0.2 M acetate buffer, and pH 4.9. After extraction, the resulting solutions were complexed with BSA and centrifuged. The precipitate was then dissolved in a solution of ...
6
TUTORIA DA ANATOMIA MAMÁRIA FEMININA UTILIZANDO UMA REDE NEURAL ARTIFICIAL INTERACTIVE ACTIVATION AND COMPETITION ORIENTADA A SERVIÇO DANIEL DE SOUZA BRAGA
... Uma RNA IAC é um modelo de paradigma conexionista apresentada por Rumelhart e McClelland no livro Parallel Distributed Processing (PDP) (CURILEM, ...A RNA IAC, conforme Figura 3, consiste em uma coleção de ...
143
UNIVERSIDADE FEDERAL DE UBERLÂNDIA FACULDADE DE MEDICINA PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE LUIZ CARLOS DE OLIVEIRA JÚNIOR
... de RNA (RNA-seq) para estudo do transcriptoma de cabeça de Drosophila melanogaster tratada cronicamente com citalopram permitiu demonstrar modificações da expressão gênica que indicam uma ação ...
73
Tamil Text Extraction
... Digital cameras have now become very popular and it is often attached with various hand held devices like mobile phones, PDAs, watches etc. Manufacturers of these devices are now a days looking for embedding various ...
4