Rna Sequencing
Differential Gene Expression (DGE) by RNA sequencing analysis and development of software for integrating different DGE methods
... three independently tomato displayed identical gene expression profiles induced by ectopic expression of T474D mutant receptor. Secondly, while 90% of the ribosomal protein genes were down-regulated; several resistant ...
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Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis
... The RNA sequencing results showed that, compared with the control strain, 1128 genes were differentially expressed in the isolate strain, and these included 227 up-regulated genes and 901 down-regulated ...
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RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in Ethanol-Treated Embryoid Bodies.
... Alcohol and developmental disorder is a well-established phenomenon but the manner in which alcohol exerts its toxic effect is still not well understood. Here, we have tried to explore genomic alterations mediated by ...
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Maize gene atlas developed by RNA sequencing and comparative evaluation of transcriptomes based on RNA sequencing and microarrays.
... using RNA sequencing ...same RNA samples originally used for our microarray-based atlas were evaluated using ...tissues. RNA-Seq provided enhanced coverage of the transcriptome, with ...by ...
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De Novo Transcriptome Assembly of the Chinese Swamp Buffalo by RNA Sequencing and SSR Marker Discovery.
... [18]. RNA sequencing (RNA-seq) is a high throughput technology that has been effectively utilized in transcriptional analysis, gene discovery, and development of molecular markers in various spe- ...
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Detection theory in identification of RNA-DNA sequence differences using RNA-sequencing.
... in sequencing technology have allowed for detailed analyses of the transcriptome at single-nucleotide resolution, facilitating the study of RNA editing or sequence differences between RNA and DNA ...
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A powerful method for transcriptional profiling of specific cell types in eukaryotes: laser-assisted microdissection and RNA sequencing.
... The acquisition of distinct cell fates is central to the development of multicellular organisms and is largely mediated by gene expression patterns specific to individual cells and tissues. A spatially and temporally ...
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Mechanism analysis of acid tolerance response of bifidobacterium longum subsp. longum BBMN 68 by gene expression profile using RNA-sequencing.
... Validation of RNA-Seq data for 15 different genes was performed by real-time PCR as described by Vandecasteele [28]. The primers of selected genes were designed based on Bifidobacterium longum subsp. longum BBMN68 ...
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Nuclear RNA sequencing of the mouse erythroid cell transcriptome.
... deep sequencing of nuclear RNAs (nucRNA-Seq) in parallel with chromatin immunoprecipitation sequencing (ChIP-Seq) of active RNA polymerase II, we compared the nuclear transcriptome of mouse anemic ...
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Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCsOP9 coculture system
... All human cells (CD34 positive or negative) derived from hPSCs can be analyzed by high-throughput single- cell RNA-sequencing in our further research, which will help us study the differ[r] ...
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High-resolution analysis of the 5'-end transcriptome using a next generation DNA sequencer.
... tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be generated in a simple and ...
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Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes.
... isolate RNA of sufficient quality (i.e., non-degraded RNA, free of impurities) and ...degrade RNA and are widespread in nature and laboratories ...with RNA isolation ...of RNA quality ...
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Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva.
... use RNA sequencing to identify genes that showed signifi- cant differential expression between young and old gingiva; ii) identify genes that are involved in gingival aging; and iii) determine whether the ...
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Epigenetic regulation of microRNA genes and the role of miR-34b in cell invasion and motility in human melanoma.
... Next-generation RNA sequencing also revealed a number of miRNAs that were differentially expressed in response to miR-34b Figure ...genomic sequencing (horizontal line at top; numbers represent ...
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A Comparison between Transcriptome Sequencing and 16S Metagenomics for Detection of Bacterial Pathogens in Wildlife.
... next-generation sequencing (NGS) approaches to survey neglected zoonotic bacteria in rodent populations: RNA-sequencing (RNA-Seq) and 16S-metagenomics, with the latter resolved via two ...
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Impact of Genomics Platform and Statistical Filtering on Transcriptional Benchmark Doses (BMD) and Multiple Approaches for Selection of Chemical Point of Departure (PoD).
... from RNA sequencing-, microarray-, and qPCR-derived gene expression data from a single study, and explored multiple approaches for selecting a single PoD from these ...used RNA extracted from the ...
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RNA-SEQ applied to the peacock blenny Salaria pavo: unveiling the gene networks and signalling pathways behind phenotypic plasticity in a littoral fish
... DNA sequencing methods coined as next-generation sequencing (NGS) technologies (Metzker, 2010; Shendure and Ji, 2008; Wang et ...However, RNA sequencing (RNA-Seq) technology remains the ...
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Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
... ribosomal RNA gene sequenc- ing for the identification of medically relevant Candida species in comparison with a standard phenotypic ...ribosomal RNA gene regions were used as tar- gets for ...The ...
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Protein-RNA linkage and posttranslational modifications of feline calicivirus and murine norovirus VPg proteins
... sense RNA molecules present within an infected cell can be > 1,000 fold lower than the corresponding positive sense RNA molecule (Vashist, Urena & Goodfellow, 2012), which even if it were linked to ...
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Functional specialization of the small interfering RNA pathway in response to virus infection.
... Our work also addresses the origin of vsiRNAs. Others have suggested that viral replication intermediates are the exclusive substrates for vsiRNA production [20,21,22,24]. Our analysis of SINV is consistent with a ...
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