Universal primers
Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
... presumed universal primers, new primer pairs have been developed targeting specific large assemblages, such as birds [12], lepidop- terans [13], or fish ...PCR primers, to date no alternatives to ...
8
Infectious Bronchitis Virus: Detection and Vaccine Strain Differentiation by Semi-nested RT-PCR
... degenerate primers (SYU+, SYU-) complementary to S1 regions and an anti-sense universal primer complementary to the beginning of S2 gene were used as universal primers in the semi- nested ...
8
UNIVERSIDADE FEDERAL DE SÃO CARLOS CENTRO DE CIÊNCIAS BIOLÓGICAS E DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM GENÉTICA E EVOLUÇÃO
... geographic distribution and genetic relationships among the studied penaeid species and the implications for conservationist approaches. Specific oligonucleotides were designed to amplify four mtDNA regions (COI, 16S, ...
137
Rev. Soc. Bras. Med. Trop. vol.40 número3
... The first dengue fever epidemic in the State of Rondônia (western region of Brazil) was recorded in 1997, without laboratory confirmation. Following this, there was an epidemic in Manaus, in the neighboring State of ...
4
Immunocapture-RT-PCR detection of Cassava common mosaic virus in cassava obtained from meristem-tip culture
... FIGURE 2 - Products of IC-RT-PCR, using Potexvirus universal primers, on RNA obtained from leaf extracts of cassava cultivar Olho Junto grown from meristem-tip culture combined[r] ...
5
New developments in pathogen detection and identification
... generic primers are designed, as for the 16S rRNA gene for prokaryotes or mitochondrial genes for ...as universal primers in a subsequent amplification by Rolling Circle Amplification or PCR and a ...
17
Influência de diferentes tratamentos de superfície na durabilidade da união entre um cimento resinoso e uma cerâmica policristalina de zircônia tetragonal parcialmente estabilizada com ítria.
... a Universal Testing Machine (1 ...of universal primers on control and air-abraded zirconia ...When universal primers were used alone, SbU presented significantly higher mean SBS ...
32
Universal tail sequence-SSR applied to molecular characterization of tropical maize
... three primers: the sense microsatellite primer with 17 extra base pairs (bp) tail in the 5’ end, these additional sequences are identical to the universal primers T3 (ATTAACCCTCACTAAAG), T7 ...
6
Vitor Fernandes Oliveira de Miranda
... The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using “universal” primers. The analysis of the products demonstrated most samples ...
12
Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence
... of primers: 1, a sense microsatellite primer, which carries, besides its specific sequence, a 17 base pair (bp) tail at its 5’ end identical to sequences of universal primers commonly used in ...
10
Family-specific vs. universal PCR primers for the study of mitochondrial DNA in plants
... these primers may be improved by the replacement of up to two nucleotides in their primary nucleotide sequences to increase their complementarity with template ...more universal primers was possible ...
22
Clássicos da literatura universal
... Gide: Os Moedeiros Falsos; Sinfonia Pastoral Hemingway: Adeus as Armas; Por quem os sinos dobram; O Sol também se levanta. J-D.[r] ...
8
Rev. LatinoAm. Enfermagem vol.25
... There is a substantive body of international evidence about the positive impact of APN roles for improving patient health outcomes, quality of care and health system eficiency. The implementation of these roles can ...
11
Quantification of trace-level DNA by real-time whole genome amplification.
... match primers to templates by competition with partially complementary ...tary primers, which could compete and interfere with the perfect match primers for ...
8
BIBLIOTECA DIGITAL UNIVERSAL :: Brapci ::
... O presente artigo resultou de um trabalho realizado sobre uma biblioteca virtual e sua importância para a preservação da memória. A Biblioteca Digital Universal (BDU) é uma biblioteca em âmbito mundial originária ...
11
Análise dos transcritos da translocação t(9;22) em Leucemia Mielóide Crônica.
... Foram analisadas 28 amostras de medula óssea, de 27 pacientes cujos dados clínicos são apresentados nas tabelas 1 e 2. Destas 28 amostras, 11 eram de homens (39%) e 17 eram de mulheres (61%). Todos os pacientes foram ...
10
LEI DA GRAVITAÇÃO UNIVERSAL
... ligado, ou seja, se um planeta ou cometa tiver energia total negativa, sua órbita será “fechada” com forma elíptica ou circular. • .[r] ...
34
Serv. Soc. Soc. número112
... O valor universal de um intelectual crítico.. The universal value of a critical intellectual.[r] ...
1
O Mito da Biblioteca Universal
... virtual universal e a ideia de biblioteca ligada a realidades locais e institucionais (que muitos consideram ...biblioteca universal em linha, a que a expansão da Web conferiu renovada ênfase, sendo ...
10