Vero cells
VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt
... In VERO cells, belt PAR is not detected with an antibody targeting long PAR chains (TNKS-1 synthesizes short chains); (2) in another renal epithelial cell line (MDCK), TNKS-1 is localized at the epithelial ...
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Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C636 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen
... the cells, which could eventually release surface mol- ecules including dengue ...detached cells from plates using cold medium and gentle scratch with plastic tips, which has been used for long to recover ...
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Overexpression of miR-484 and miR-744 in Vero cells alters Dengue virus replication
... into the plasmid pEGFP-C1 and the respective constructs, pGUD1, pGUD2, and pGUD4 were obtained. These con- structs were subsequently transfected into Vero cells; the empty pEGFP-C1 vector served as the ...
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Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens
... Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. ...
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Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over.
... in Vero cells. Vero cells were transfected with cDNA encoding GFP-L-plastin phosphorylation ...hours, cells were fixed and processed for ...a Vero cell transfected with WT ...
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BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
... line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human ...the cells, are being increasingly ...of Vero cells at ...
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Parameters affecting cellular invasion and escape from the parasitophorous vacuole by different infective forms of Trypanosoma cruzi
... target cells or infective forms were observed, we consistently found that amastigotes tended to escape faster from the parasitophorous vacuole since after 12 h, more than 90% of the amastigotes were already free ...
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Anticancer activity of rutin and its combination with ionic liquids on renal cells
... cancer cells and that at 50 µM, it may be safely used against ccRCC since, at this concentration, no significant effect was observed in normal renal ...in Vero cells enhanced the drug solubility ...
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Mat. Res. vol.12 número3
... inoculation. Vero cells, a fibroblast lineage were used. These cells were cultured in Ham F-10 medium with 10% fetal calf serum (FCS; Nutricell, Campinas, SP, Brazil) at 37 ...°C. Vero ...
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Mat. Res. vol.18 número3
... cultured cells provided indirect cytotoxicity showing growth pattern expected to Vero cells, and consistent with control ...The cells had become quite spread, forming a conluent monolayer with ...
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4-Phenyl-1,3-thiazole-2-amines as scaffolds for new antileishmanial agents
... THP-1 cells. Moreover, no cytotoxic effect was observed against Vero cells at the same concentration range tested, except for compound 1, which had a SI of ...
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Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium
... After stabilization was achieved, a protocol consisting of three consecutive I/R cycles (10 and 20 min, respec- tively) was initiated. During each ischemic cycle, deoxygen- ated complete MEM (1.8 mM calcium chloride, ...
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Antiviral activity of a small molecule deubiquitinase inhibitor occurs via induction of the unfolded protein response.
... (A–D) Cells were treated with DMSO, 3 mM thapsigargin (Thapsi), 5 mM WP1130, ...Be2-c cells is inhibited by WP1130 or thapsigargin. Treated Be2-c cells were infected with La Crosse virus (MOI 5) for ...
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Cytotoxicity of Southeast Asian snake venoms
... CTC 50 of ES was not detectable as it failed to show cytotoxicity towards either MDCK or Vero cells. These results might be attributable to the fact that sea snakes contain potent neurotoxins whereas 60% of ...
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CdtR Regulates TcdA and TcdB Production in Clostridium difficile.
... Unexpectedly, Western blots performed using TcdA- and TcdB-specific antibodies showed that the cdtR mutants produced less TcdA and TcdB than the wild type, while the comple- mented cdtR derivatives expressed high levels ...
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Recombinant Yellow Fever Viruses Elicit CD8(+) T Cell Responses and Protective Immunity against Trypanosoma cruzi
... Chagas’ disease is a major public health problem affecting nearly 10 million in Latin America. Despite several experimental vaccines have shown to be immunogenic and protective in mouse models, there is not a current ...
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A novel live-attenuated vaccine candidate for mayaro Fever.
... vertebrate cells, but not in invertebrate cells, the translation of viral structural proteins was placed under control of the EMCV IRES, directly downstream from the subgenomic ...into Vero ...
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Identification of novel surface-exposed proteins of Rickettsia rickettsii by affinity purification and proteomics.
... infected Vero cells were treated with fixing agent (4% parafor- maldehyde, ...the cells were sequentially dehydrated with 50%, 70%, 85%, and 95% alcohol and successively permeated in a mixture of LR ...
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PATHOGENICITY OF Entamoeba dispar UNDER XENIC AND MONOXENIC CULTIVATION COMPARED TO A VIRULENT
... Two xenic isolates and cloned cultures of Entamoeba dispar were submitted to monoxenization using Crithidia fasciculata as the associated organism. Growth in monoxenic cultivation and ability of xenic and monoxenic ...
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Use of blends of bioabsorbable poly(L-lactic acid)poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture
... flattened cells were ...40) cells were flat, possibly because of the effects of surface ...of Vero cells on pure PLLA scaffolds observed here was similar to previ- ous results obtained by our ...
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