[PDF] Top 20 Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
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Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
... from clinical specimens and bacterial strains were isolated as previously described (4), with some modifications: bacterial strains or pellet from 400ml of treated sputum were washed in 10x TE (1x TE ... See full document
4
NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION
... Clinical samples: The sensitivity and specificity of primers FLC2/ RLC2 were analyzed in clinical samples: lesion biopsy and blood from a dog with clinical suspicion ... See full document
8
Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures
... frozen samples in selenite cystine broth were again subjected to bacterial enrichment using 9mL of selenite-cystine ...isolated by boiling lysis (Santos et al., 2001). A 400μL sample was ... See full document
8
One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
... EB in the reaction mix- ture. However, the sensitivity under clinical test condi- tions could be lower due to PCR inhibitors not completely removed during the DNA ...secretion samples found ... See full document
4
Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
... diagnosis of MHV infection is mainly performed by serologic assays because of the difficulties in finding recognized histological lesions and in isolating the virus in tissue ... See full document
8
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems
... case of internal PCR (WbF/Wb2) detection, it was not possible to make a comparison with Kanja- navas et ...the detection limit. However, the detection limit found was higher for other authors ... See full document
6
Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction- single-strand conformation polymorphism and direct sequencing methods
... centers in Iran from 2002 to 2004. All isolates were characterized by conventional methods (Staining by Ziehl Neelsen method, observation of speed of growth and colony morphology and ... See full document
6
Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples
... analyzed samples were stored from 1999 to 2006. These samples were also evaluated considering the time of storage in order to determine the influence of the freezing time in the ... See full document
6
A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
... amplification of mycobacterial DNA in clinical samples. Use of polymerase chain reaction for rapid diagnosis of tubercu- losis. Detection of Mycobacterium tuberculosis in spu- tum sample[r] ... See full document
7
Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
... children in the state of São Paulo (SP). Another study conducted in SP revealed the presence of ...hominis in all 29 stool samples analysed during an outbreak at a day care ... See full document
4
Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
... established in 61.9% by the conven- tional method and in ...aethiopica in paraffin-embedded tissue, in 7 out of the 22 cases where the conventional methods ...cases of the ... See full document
6
Development and application of polymerase chain reaction test for detection of Conidiobolus lamprauges
... caused by the fungus Conidiobolus spp. in humans and ...diagnosis of the disease is isolation of the agent associated with the presence of typical clinical signs and pathological ... See full document
5
Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis
... aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain ... See full document
3
Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
... efficiency of DNA amplification is decreased in tissue sections in comparison to fluid phase PCR (Komminoth et ...However, in situ PCR has the advantage of localizing target sequences ... See full document
4
Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
... developed in Vitória, Southeast Brazil, from a set of biological samples ob- tained over a two-year period (August 2007-August 2009) as a part of the Respiratory Virus Surveillance Program ... See full document
6
Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction
... onset of disease. At that time demonstration of anti-dengue IgM antibody was a good marker to confirm the clinical and epidemiological diagno- ...successful in detecting DEN-1 virus in ... See full document
5
Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction
... lack of informativity from heterozygotic individuals is one of the difficulties in the QTL mapping of outbred populations (Liu, ...omitted in the analyses of mean contrasts, and ... See full document
9
Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction
... genome of Indiana 3-Alagoas virus was amplified from the clinical samples of infected guinea pig, detecting viremia and virus presence in the animal tarsal pad epithe- lial ...diagnosis ... See full document
4
A diagnosis of Burkholderia pseudomallei directly in a lavage by polymerase chain reaction
... morphology of some ...especially in first-line laboratories equipped to perform DNA amplification methods such as diagnostic polymer- ase chain reaction (PCR) (Merritt et ...most of ... See full document
3
DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
... pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern ...dissection ... See full document
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