[PDF] Top 20 Extraction of DNA from honey and its amplification by PCR for botanical identification
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Extraction of DNA from honey and its amplification by PCR for botanical identification
... produced from plant nectar after being modified by different substances produced by honeybees by dehydration and by maturation in the ...original DNA extraction ... See full document
4
Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee
... isolate and identify Lactobacillus in the honey stomach of honeybee Apis ...Samples of honeybee were collected from ...trees and Lactobacillus bacteria isolated from ... See full document
6
Accurate Identification of Candida parapsilosis (Sensu Lato) by Use of Mitochondrial DNA and Real-Time PCR
... frequently from blood cultures in South America and some European countries, such as ...complex of 3 closely related species: ...metapsilosis, and Candida orthopsilosis. Here, we describe a ... See full document
5
Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis
... confirm and validate the results of HRM analysis, the PCR products of the 11 Lavandula specimens amplified with the Lav1-F/Lav1-R primers were sequenced in opposite directions, obtaining ... See full document
7
Characterization of family IV UDG from Aeropyrum pernix and its application in hot-start PCR by family B DNA polymerase.
... thermostable DNA polymerases are used to amplify DNA, and their enzymic characteristics decide whether or not a PCR- reaction with special amplification performance can be ...discovery ... See full document
10
Extraction of viral DNA PCR qPCR: The viral genomes present
... µM of dGTP, 400 µM dATP, 400 µM dCTP, 800 µM dUTP, uracil DNA glycosilase (UDG)], and ...µL of distilled water free from RNAses (RNAse/DNAse free water system, Merck-Millipore, ... See full document
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COMPARATIVE STUDY OF DNA EXTRACTION TECHNIQUES AND ITS USE IN THE POLYMERASE CHAIN REACTION (PCR) FOR THE DIAGNOSIS OF Theileria equi AND Babesia caballi.
... equi and Babesia caballi are the causing agents of equine ...mitted by ticks present in areas that range 90% of the world equine ...diagnosis of equine babesiosis might be done ... See full document
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COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
... DNA by adding an equal volume of phenol-chloroform to aqueous solution of lysed cells, mixing the phases and allowing them to separate by ...extracted DNA is precipitated ... See full document
5
Towards honey authentication: assessing European honey entomological origin by a molecular identification approach
... work, DNA markers were developed for the differentiation of ...subspecies DNA in ...individuals of A. m. iberiensis lineage A (n=22) from Portugal and Spain (n=5), ...M ... See full document
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PCR AMPLIFICATION AND SEQUENCING
... DNA extraction was performed from a similar protocol to that proposed by Castalanelli et ...hydrolysis of proteins. The procedure consists of introducing the complete specimen ... See full document
7
Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification
... performance of PCR ribotyping and tDNA-PCR for identification of ...these PCR methods were evaluated. No polymorphism was detected in the 16S and 23S ISRs among ... See full document
6
Identification of canine papillomavirus by PCR in Greyhound dogs
... characterized by well-defined conical hyperkeratosis that project above the skin surface with no evidence of viral cytopathology or inflammation (Gross et ...immunohistochemistry and PCR were ... See full document
7
Comparison of Different Methodologies for DNA Extraction from Aegla longirostri
... attempts of DNA extraction from ...addition of β-mercaptoethanol to the lysis buffer (Protocol ...damage of nucleic acids (Herzer, ...quantity of DNA on agarose ... See full document
6
Protocol for extraction of genomic DNA from swine solid tissues
... performed by using DNA from different body ...genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA ... See full document
3
DNA mini-barcodes coupled to high resolution melting (hrm) analysis for the botanical authentication of rosemary honey
... for its taste, nutritional value and health ...appreciated by consumers and frequently attain high market values, thus being prone to fraudulent ...development of methodologies to ... See full document
14
Evaluation of methods for the extraction and purification of DNA from the human microbiome.
... ability of six DNA extraction methods previously used in studies of the human microbiome and environmental samples to recover DNA from known organisms and yield ... See full document
10
Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD)
... development of in vitro propagation of cells has been an extraor- dinary technical advance for several biological ...correct identification of the cell line used, however, is crucial, as a ... See full document
6
Use of real-time PCR to evaluate two DNA extraction methods from food
... Smith and Maxwell (2007) compared real-time PCR efficiences of invr1 gene amplification using DNA extracted using four different methods from corn flour, and observed that ... See full document
7
A mechanism of gene amplification driven by small DNA fragments.
... hyg, and the counter- selectable KIURA3 marker gene) and the I-SceI site (HOT site) in TRP5 ...KM-193 and KM-196 are two independent isolates derived from strain FRO-155 by replacing ... See full document
14
Improving DNA isolation from honey for the botanical origin identification
... use of DNA markers present in pollen to speci fically identify the botanical species of honey is a novel and promising ...use of DNA-based methods offer advantages ... See full document
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