[PDF] Top 20 Extraction of viral DNA PCR qPCR: The viral genomes present
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Extraction of viral DNA PCR qPCR: The viral genomes present
... collection: The municipalities of Rolante and Riozinho, located in Vale do Paranhana, Rio Grande do Sul, Brazil, have most of its population living in small farms, and their economy is based on dairy ... See full document
6
RNA extraction, RT and Real-Time PCR (qPCR)
... by qPCR (Ct between 35 and 39). In most cases the hemagglutination test did not produced positive ...described the difficulty of isolating NDV from clinical specimens from healthy adult birds, ... See full document
7
Genome-wide analysis of EgEVE_1 , a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes
... Endogenous viral elements (EVEs) are the result of heritable horizontal gene transfer from viruses to ...In the last years, several EVE integration events were reported in plants by the ... See full document
9
Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.
... in the absence of target, especially after 40 cycles of PCR, is problematic in clinical diagnostic tests where RNA target copies may approach single molecule ...since the retroviral RTs ... See full document
13
Rev. Soc. Bras. Med. Trop. vol.50 número3
... for the accurate detection of infectious and parasitic diseases, such as the ...leishmaniases. The present study describes the optimization and evaluation of a duplex ... See full document
8
Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.
... droplet PCR for total HIV-1 DNA. Cellular DNA was extracted using a Qiagen DNA Blood Midi Kit, following the manufacturer’s ...protocol. DNA was ethanol precipitated following ... See full document
17
COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
... in the present study four commercially available dNa extraction kits (Wizard® Genomic dNa Purification Kit, High Pure PCr Template Kit, dNeasy mericon Food and GenejeT PCr ... See full document
5
A search for RNA insertions and NS3 gene duplication in the genome of cytopathic isolates of bovine viral diarrhea virus
... genome of cpBVDVs 26, 44, and 63 harbors insertions in the NS2-3 gene which are indistinguishable in size from the inser- tion present in the NADL strain, as ascer- tained by agar gel ... See full document
10
DNA extraction, PCR, and DNA sequencing
... species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and ...existence ... See full document
9
Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.
... on the virus strain, mosquito species, environmental conditions, and infection status (nondisseminated limited to the midgut or disseminated throughout the mosquito’s body) ...Dilutions of ... See full document
16
Amplificação da sequência de inserção IS6110 diretamente do esfregaço das lâminas coradas para bacilo álcool- ácido resistente
... U) of Taq DNA Polymerase Invitrogen, in a final volume of 50 ...μL. The initial DNA denaturation was at 94°C for 2 min, following a total of 40 cycles of 94ºC for 30 sec, ... See full document
60
Diagnóstico de infecção congênita e perinatal por citomegalovírus utilizando a reação em cadeia da polimerase.
... Abstract The practical application of a polymerase chain reaction (PCR) amplification for the diagnosis of congenital and perinatal cytomegalovirus (CMV) infections was ...by PCR ... See full document
8
Winning the Oil Lottery: The Impact of Natural Resource Extraction on Growth Tiago Cavalcanti Daniel Da Mata Frederik Toscani
... that the whole of Brazil went through a process of moving towards a higher share of services, a lower share of agriculture, more urbanization, and less ...sample of ... See full document
50
Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection.
... estimate the survival of the wild-type and TLR6 -/- mice population after DV infection for over 21 ...days. The survival plots of the DV2-infected wild-type and TLR6 -/- mice ... See full document
34
Role of baseline HIV-1 DNA level in highly-experienced patients receiving raltegravir, etravirine and darunavir/ritonavir regimen (ANRS139 TRIO trial).
... Funding: The authors thank Merck Sharp & Dohme-Chibret for providing raltegravir; and Tibotec, a division of Janssen-Cilag, for providing ...etravirine. The research leading to these results has ... See full document
3
REVIEW THE INFLUENCE OF THE HUMAN GENOME ON CHRONIC VIRAL HEPATITIS OUTCOME
... in the tools used in the field of molecular ...in the human genome, as well as facilitating the determination of correlation between such polymorphism and disease ...mechanisms. ... See full document
8
The intrinsic antiviral defense to incoming HSV-1 genomes includes specific DNA repair proteins and is counteracted by the viral protein ICP0.
... highlight the emerging parallels between cellular recognition of viral DNA and the cellular response to DNA ...by the ubiquitin ligases RNF8 and RNF168, and ICP0 is thus ... See full document
12
Epidemiology and Clinical Characteristics of Respiratory Infections Due to Adenovirus in Children Living in Milan, Italy, during 2013 and 2014.
... evaluate the predominant human adenovirus (HAdV) species and types associated with pediatric respiratory infections, nasopharyngeal swabs were collected from otherwise healthy children attending an emergency room ... See full document
13
Evaluation of the Binax NOW Flu A+B Enzyme Immunochromatographic Assay in comparison with Real-Time PCR during the Pandemic of Influenza 2009
... push the importance of the presence of these imunochromatografic assays in hospital or also in different points of ...performed the rRT-PCR in comparison with Binax NOW ... See full document
4
ATM and ATR activities maintain replication fork integrity during SV40 chromatin replication.
... structures of viral replication intermediates generated in the presence and absence of ATR kinase activity were characterized by using neutral 2 d gel electrophoresis and southern ... See full document
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