[PDF] Top 20 Intraclonal protein expression heterogeneity in recombinant CHO cells.
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Intraclonal protein expression heterogeneity in recombinant CHO cells.
... particularly in culture [77], resulting in an enormous diversity of physiological states, which may also have an indirect effect on transgene ...enables cells to adapt to short-lived environmen- tal ... See full document
11
Establishing a High Titer Transient Gene Expression Process in Conditioned Media for CHO-DG44 Cells
... (WPRE) in the 3’UTR region of the transgene mRNA (Baldi et ...Mammalian cells expressing the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1), like the HEK293-EBNA1 cell line, are capable of episomal ... See full document
55
Expressão da proteína L1 do capsídio de HPV-16 em leveduras metilotróficas
... epithelial cells and can cause both benign and malignant ...capsid protein can self- assemble into virus-like particles (VLPs), which are morphologically and antigenically indistinguishable from native ... See full document
106
Immunization with cDNA expressed by amastigotes of Trypanosoma cruzi elicits protective immune response against experimental infection
... purified recombinant protein (His-25kDa) in complete Freund’s adjuvant (CFA) in the hind ...Eukaryotic expression vectors: plasmid generation, purification, and immu- ...(details ... See full document
14
A DNA enzyme targeting Egr-1 inhibits rat vascular smooth muscle cell proliferation by down-regulation of cyclin D1 and TGF-β1
... The catalytic DNA, ED5, and scrambled control DNA enzyme, ED5SCR, were synthesized by Takara (China). The sequences were as follows: ED5 5’-CCGCTGCCAGG CTAGCTACAACGACCCGGACGT-3’; ED5SCR 5’-GCC ... See full document
9
Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures
... of recombinant L-PGDS ...USA). Cells were ground using liquid nitrogen and a ...ground cells in 1:1 ratio (g of cells: mL of extraction ...resolved in a ...Portugal) in ... See full document
9
Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang and Lin Hou
... role in the synthesis of n-3 PUFAs, since it can efficiently covert n-6 PUFAs to n-3 ...functions in humans. However, its content in the diet is often ...insufficient. In plants, n-3 FAD ... See full document
10
Orientador: Doutora Lídia Maria Diogo Gonçalves, Co-orientador: António José Leitão Neves Almeida, Professor Catedrático, Faculdade de Farmácia da Universidade de Lisboa
... specific recombinant antigens, but previous studies in animal models provided poor or no immunity against ...that recombinant protein is expressed in both prokaryotic and eukaryotic ... See full document
87
Extraction of matrine from Sophora flavescens Ait. and evaluation of its inhibitory effects on human nasopharyngeal carcinoma CNE-2 cells
... CNE-2 cells and expressions of Bac-2 and caspase-3 protein in cells were ...CNE-2 cells, promote the cell apoptosis, and arrest most cells in G0/G1 ...relative ... See full document
5
Produção do fator estimulador de colônias de granulócitos humano recombinante (rhG-CSF) em biorreator
... cultivation in bioreactor. The protein expression was analyzed by SDS-PAGE 15% and the gel was stained with Coomassie Brilliant ...concentration in the medium was measured with an YSI glucose ... See full document
59
Francisca Monteiro PhD thesis
... Sf9 cells, at least in such an ...Five cells have a low pyruvate carboxylase (PC) ...succeed in diminishing lactate accumulation. In fact, what is limiting the metabolization of ... See full document
228
Lipid modifications of Sonic hedgehog ligand dictate cellular reception and signal response.
... identified in our in vitro assays may explain opposing and seemingly puzzling effects on limb patterning observed in prior in vivo studies following expression of Shh lacking either the ... See full document
12
Global Identification of EVI1 Target Genes in Acute Myeloid Leukemia.
... role in cell cycle regulation ...retinoblastoma protein (Rb), a tumor suppressor that prevents excessive cellular division ...Serpinb2 expression to surprisingly high levels (up to 10,000- fold) ... See full document
18
Modulating drug resistance by targeting BCRP/ABCG2 using retrovirus-mediated RNA interference.
... virus-packaging cells, Escherichia coli TOP10 cells, Stbl3 competent cells, pENTR/U6, and the shRNA retrovirus vector pLenti6/BLOCK-iT-DEST were purchased from Invitro- gen (Carlsbad, CA, ...raised ... See full document
9
J. Appl. Oral Sci. vol.23 número3
... used in this study as a control to observe whether there were differences in the staining pattern of DLS in this group in comparison to group ...DLS in the glands of young individuals ... See full document
10
Intratumoral Heterogeneity of MicroRNA Expression in Rectal Cancer.
... intratumoral heterogeneity is ...intratumoral heterogeneity of a panel of miRNAs in breast cancer [18] by ...intratumoral heterogeneity can lead to significant sampling bias and, assessment of ... See full document
13
Recombinant proteins in differentiation of stem cells
... resuspended in 10 mL of ice cold STE Buffer (10 mM Tris-HCl, pH ...chromatography in a fast protein liquid chromatography (FPLC) ÄKTA TM (Amersham Biosciences), and all the process was monitored by ... See full document
90
Ca2+ influx and tyrosine kinases trigger Bordetella adenylate cyclase toxin (ACT) endocytosis. Cell physiology and expression of the CD11b/CD18 integrin major determinants of the entry route.
... fundamental in the toxin uptake by all the cell types studied ...mechanism in non-excitable cells, such as oocytes ...of cells to membrane ruptures induced either by mechanical forces as well ... See full document
14
Role of protein tyrosine phosphatase non-receptor type 7 in the regulation of TNF-α production in RAW 264.7 macrophages.
... pathways in innate immune cells are rapidly activated by phosphorylation, inducing a pro-inflammatory cytokine response to LPS of gram-negative bacteria, and are then down- regulated by the ... See full document
7
Long-time treatment by low-dose N-acetyl-L-cysteine enhances proinflammatory cytokine expressions in LPS-stimulated macrophages.
... IL-6 in LPS stimulated RAW264.7 cells, whereas high-dose NAC treatments decreased their ...expressions in LPS-stimulated ...consequences in phosphorylation levels of several kinases, includ- ... See full document
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