[PDF] Top 20 MATERIALS AND METHODS Microorganism and media

... production and productivity of secondary metabolites by cell culture technology is still facing many biological and engineering ...production and high mycelial growth rate ...statistical ... See full document

... hull and rice husk), fruit juice wastes (sugarcane bagasse, orange peel and orange pulp) and edible oil industries waste (cottonseed oil cake and groundnut oil cake) to check their suitability ... See full document

... growth media with intermediate fatty acids in the form of oil precursors and their subsequent conversion to desired long chain fatty acids offers a better approach for studying their effect on desired fatty ... See full document

... characteristics and enzyme production were ...husk and bird performance characteristics when employed as poultry diet additive were ...6.0 and 55 o ...Tween-80 and SDS yielded endoglucanase ... See full document

... methanol and distilled water as described by (15). A mixture of dried cells and solvents was sonicated in a sonication bath for 15 min, and then the mixtures were incubated at room temperature for 18 ... See full document

... nature and the alkalophilic Bacillus strains are often good sources of alkaline extracellular enzymes (11, ...actinomycetes and bacteria (13, ...fat and protein debris by chemical processes is both ... See full document

... from Bacillus sp SMIA-2 retained more than 70% activity after incubated for 1 h at 50ºC with sodium dodecyl sulphate, an anionic detergent (Fig. 7). However, very little residual activity was obtained with sodium ... See full document

... psychrophiles and psychrotrophs inhabit both terrestrial and aquatic environments in polar and alpine regions, in the bulk of the ocean, in shallow subterranean regions, in the upper atmosphere, in ... See full document

... because it was too large to have arisen by chance by itself. In this case, p≤0.05 suggested significance at the 0.05 level. This also corresponded to a 95% of confidence level for test of the hypothesis that the effects ... See full document

... DEAE-Cellulose and CM-Cellulose chromatography, and purified 141-fold, with ...Cellulose and Concanavalin A-Sepharose chromatography, with ...Temperature and pH optima were in the range of ... See full document

... exploitation and food industries, which are treated or sent to sanitary ...economic and environmental aspects, the reuse of agricultural wastes has taken on an extremely important dual purpose: elimination ... See full document

... solution and the oil. As the oil remains for longer times, the physical and chemical nature of the soil becomes modified, as noted by Urum et ...components and oil of lower molecular weight, ... See full document

... No inhibitory effect was observed at 1, 2, 5 and 10 mM of EDTA. However, with 10 mM the enzyme retained about 84% of its activity when incubated for 30 min. This finding was similar to that of Tunga et al. (19), ... See full document

... pH and UV light at longer time points, suggesting that despite the fact that the nadB gene is not essential for Caulobacter, its loss causes it to be more sensitive to some ... See full document

... concentration and metabolic activities (Tibayrenc et ...well-defined media, is under ...target microorganism and distinguishing between different microbial populations, as exist in practical ... See full document

... Growth and fermentation of sugarcane juice to ethanol by KO11 and P2 required addition of ...micronutrients and thiamine promoted better ethanol ...g/L, and a reduction in biomass production ... See full document

... 3 and 4), presented the same degree of open ...refractory materials, like alumina (ABNT; MB-67); the average values for the spheres were 108,7 % for 244 and 108,6% for 245, what strongly supports ... See full document

... NA1000, and normal swimming behaviour was observed by light ...phase, and cell viability was ...PepF and ORF3 are not essential for cell viability during prolonged ...presence and absence of ... See full document

... SIGMA, St. Louis, USA) (ε420 = 3.6×10 4 M cm −1 ) (1) . The reaction mixture contained 100 Ql substrate, 800 Ql of buffer 50 mM sodium acetate (pH 5.0 ) and 100 L of the culture supernatant (diluted when ... See full document

... dichloromethane, and DDT in the soils was extracted with an equal volume of hexane and acetone (1:1, v/v) by the supersonic extraction ...sulfate, and then evaporated using a vacuum rotary evaporator ... See full document