Top PDF Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.
Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.
... Adult human dermal fibroblasts endogenously expressed Klf4 and c-Myc but not any other genes (data not ...,F and iPS cell colony G also expressed Klf4 and c-Myc, indicating that these two ...
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Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes.
... the expression levels of apoptotic-related ...Bcl2 and Bax genes were confirmed with human ventricular tissue as well as with hiPSC-CMs by ...the gene expression levels of Caspase 3, ...
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The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
... selection criteria were again p < 0.05 (Student’s t-test) and at least a two-fold difference in gene ...lines and the original cell ...hundred and fifty-nine genes in this set (32%) ...
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Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
... Reprogramming and Characterization Induced pluripotent stem cell lines were derived from primary skin cells obtained from skin ...F050914007, and the Informed Consent is approved ...
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DNA methylation dynamics in human induced pluripotent stem cells over time.
... modification and is a key component in normal differentiation, development and disease ...[1–3]. Expression of tissue-specific genes, such as Oct-4 [4], Nanog [5], Sry (sex determining region on Y ...
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Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination.
... PCR and immunostaining data has shown that hiPSCs share similarities in gene expression patterns with hESCs during differentiation to ...antigen-expressing cells measured by immuno- ...
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Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells.
... of cells to be the percentage of genes that are differentially expressed between them; thus, two ‘‘closer’’ groups will have a lower percentage of genes that are different between them, and vice ...After ...
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Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts.
... low expression of MHC-I and negative MHC-II, costimulatory molecules [25, 27, ...that human iPSCs have low MHC-I gene expression, un-detectable MHC-II expression [29], here we ...
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Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression.
... hESCs/hiPSCs and demonstrated VCAM1 as a potent surface marker for cardiomyocytes induced with this ...embryo and hESCs ...selective and non-genetic method to prepare cardio- myocytes for ...
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Genetic background drives transcriptional variation in human induced pluripotent stem cells.
... estimates and correlation heatmap were qualitatively very similar when the top 1 and 5% of genes were removed from the data set (Figure S7, ...IPS and ES cells (Figure ...sample gene ...
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Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells
... iPSC and ES cell aggregates, at days 8 and 14 of differentiation using TRIzol Reagent (Invitro- gen, ...UK) and random hex- amer primer, following manufacture’s ...USA) and analyzed with the ...
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Scalable passaging of adherent human pluripotent stem cells.
... solution. Cells were counted, distributed into tubes, and ...6 cells were suspended in hESC-Qualified Matrigel TM ...daily, and the tumor measured twice a week by veterinary services staff ...
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Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells.
... discovery, and the study of disease mechanisms ...Choi and his colleagues showed similar patterns of differentiation potential between human iPSC lines and hESC lines, as determined using an ...
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Induced pluripotent stem cells, a giant leap for mankind therapeutic applications
... Induced pluripotent stem cells (iPSC) technology has propelled the field of stem cells biology, providing new cells to explore the molecular mechanisms of pluripotency, ...
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MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor.
... TGF-beta and MEK signaling pathways are sufficient for reprogramming towards pluripotency, primary cell culture from CF-1 mouse em- bryo at ...μM) and TGF-beta (SB431542; 2 μM) (See Fig 1A and ...
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Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
... The cells exhibited traditional human ES/iPS morphology with a high nuclear to cytoplasmic ratio ...5 cells we routinely used to start reprogramming, the number of human ES like colonies ...
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Targeted gene therapy of xeroderma pigmentosum cells using meganuclease and TALEN™.
... locus and rescue its nuclease ...293-H cells (12-fold, 12% HGT, figure ...XP4PA cells treated with 5-aza-dC displayed an altered cellular proliferation compared to their untreated coun- ...line ...
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Expression and secretion of human recombinant LIF by genetically modified mammalian cells
... Gene expression in human neural stem cells: effects of leukemia inhibitory factor // J.. Embryonic stem cells capable of differen- tiating into desired cell lines / J.[r] ...
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Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.
... the human genome has recently been measured to be 50–70 de novo single nucleotide variants (SNVs) between ...somatic cells so that an organism is a ...tissue and between tissues has not been ...
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Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.
... reprogrammed cells, making use of the loxP sites included in the proviral LTRs ...of cells with normal karyotype (Table 2), was co-electroporated with Cre recombinase- encoding pMC-Cre plasmid and ...
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