[PDF] Top 20 Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.

... Adult human dermal fibroblasts endogenously expressed Klf4 and c-Myc but not any other genes (data not ...,F and iPS cell colony G also expressed Klf4 and c-Myc, indicating that these two ... See full document

... the expression levels of apoptotic-related ...Bcl2 and Bax genes were confirmed with human ventricular tissue as well as with hiPSC-CMs by ...the gene expression levels of Caspase 3, ... See full document

... selection criteria were again p < 0.05 (Student’s t-test) and at least a two-fold difference in gene ...lines and the original cell ...hundred and fifty-nine genes in this set (32%) ... See full document

... Reprogramming and Characterization Induced pluripotent stem cell lines were derived from primary skin cells obtained from skin ...F050914007, and the Informed Consent is approved ... See full document

... modification and is a key component in normal differentiation, development and disease ...[1–3]. Expression of tissue-specific genes, such as Oct-4 [4], Nanog [5], Sry (sex determining region on Y ... See full document

... PCR and immunostaining data has shown that hiPSCs share similarities in gene expression patterns with hESCs during differentiation to ...antigen-expressing cells measured by immuno- ... See full document

... of cells to be the percentage of genes that are differentially expressed between them; thus, two ‘‘closer’’ groups will have a lower percentage of genes that are different between them, and vice ...After ... See full document

... low expression of MHC-I and negative MHC-II, costimulatory molecules [25, 27, ...that human iPSCs have low MHC-I gene expression, un-detectable MHC-II expression [29], here we ... See full document

... hESCs/hiPSCs and demonstrated VCAM1 as a potent surface marker for cardiomyocytes induced with this ...embryo and hESCs ...selective and non-genetic method to prepare cardio- myocytes for ... See full document

... estimates and correlation heatmap were qualitatively very similar when the top 1 and 5% of genes were removed from the data set (Figure S7, ...IPS and ES cells (Figure ...sample gene ... See full document

... iPSC and ES cell aggregates, at days 8 and 14 of differentiation using TRIzol Reagent (Invitro- gen, ...UK) and random hex- amer primer, following manufacture’s ...USA) and analyzed with the ... See full document

... solution. Cells were counted, distributed into tubes, and ...6 cells were suspended in hESC-Qualified Matrigel TM ...daily, and the tumor measured twice a week by veterinary services staff ... See full document

... discovery, and the study of disease mechanisms ...Choi and his colleagues showed similar patterns of differentiation potential between human iPSC lines and hESC lines, as determined using an ... See full document

... Induced pluripotent stem cells (iPSC) technology has propelled the field of stem cells biology, providing new cells to explore the molecular mechanisms of pluripotency, ... See full document

... TGF-beta and MEK signaling pathways are sufficient for reprogramming towards pluripotency, primary cell culture from CF-1 mouse em- bryo at ...μM) and TGF-beta (SB431542; 2 μM) (See Fig 1A and ... See full document

... The cells exhibited traditional human ES/iPS morphology with a high nuclear to cytoplasmic ratio ...5 cells we routinely used to start reprogramming, the number of human ES like colonies ... See full document

... locus and rescue its nuclease ...293-H cells (12-fold, 12% HGT, figure ...XP4PA cells treated with 5-aza-dC displayed an altered cellular proliferation compared to their untreated coun- ...line ... See full document

... Gene expression in human neural stem cells: effects of leukemia inhibitory factor // J.. Embryonic stem cells capable of differen- tiating into desired cell lines / J.[r] ... See full document

... the human genome has recently been measured to be 50–70 de novo single nucleotide variants (SNVs) between ...somatic cells so that an organism is a ...tissue and between tissues has not been ... See full document

... reprogrammed cells, making use of the loxP sites included in the proviral LTRs ...of cells with normal karyotype (Table 2), was co-electroporated with Cre recombinase- encoding pMC-Cre plasmid and ... See full document