[PDF] Top 20 Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.

... RE19 from S7) carried fewer mutations, of which a subset was shared between them as well as with EPCs from the same ...None of the shared SNVs were detected in the corresponding fibroblasts or ... See full document

... three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including human ... See full document

... groups of cells to be the percentage of genes that are differentially expressed between them; thus, two ‘‘closer’’ groups will have a lower percentage of genes that are different ... See full document

... U.K.). Cells plated on glass coverslips were incubated in β50µl of culture medium with a final concentration of 1µl of fura-β-AM (Life Technologies, Paisley, Strathclyde, ...re-emission ... See full document

... The cells exhibited traditional human ES/iPS morphology with a high nuclear to cytoplasmic ratio ...5 cells we routinely used to start reprogramming, the number of human ... See full document

... CD34+ cell population had hematopoietic and endothelial cell ...these cells were subjected to the hematopoietic colony-forming cell assay, various hematopoietic CFU colonies were ... See full document

... rejection of syngeneic mouse iPSCs (miPSCs) following transplantation [17], in which miPSCs derived via the episomal approach were less prone to immune-mediated attack than those generated using viral ... See full document

... Characterization of human induced pluripo- tent stem ...Primary human keratinocytes (PHK) derived from skin biopsy were reprogrammed to iPSC with a modified version ... See full document

... Expression of tissue-specific genes, such as Oct-4 [4], Nanog [5], Sry (sex determining region on Y chromosome) [6] and MyoD [7], are induced by spatio-temporal demethylation during ...and cell ... See full document

... comprised of iPSC-derived fibroblasts, keratinocytes and melanocytes exhibited relatively normal tissue ...homed to the basal layer of the epidermis and produced melanin in this ...ratio of ... See full document

... Advancing pluripotent stem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing Practices- ... See full document

... capacity of mammals is limited and can rarely regenerate a specific organ or tissue ...Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of ... See full document

... function of the nervous system is maintained via well-coordinated processes both in central and peripheral nervous tissues, which govern the homeostasis of organs/tis- ...Impairments of the nervous ... See full document

... Adult human dermal fibroblasts endogenously expressed Klf4 and c-Myc but not any other genes (data not ...iPS cell colony G also expressed Klf4 and c-Myc, indicating that these two genes were expressed ... See full document

... source of cells for ...original cells reprogrammed; this precludes the need for complex procedures to create the genetic defect and avoids potential secondary effects of the genetic ... See full document

... Induced pluripotent stem cells (iPSCs) are derived from somatic cells by transfecting two pluripotent tran- scription factors, Oct4 (O) and Sox2 (S), and two proto- ... See full document

... invulnerable to the passage of years, the impact of age on oocytes being consistent with its effect on the risk of congenital abnormalities (Balen, ...contain cells which can be ... See full document

... led to efficient motoneuron generation from iPS and E14 cell ...ALS-iPS-1 cell culture after 5 days treatment with RA and SHH ...found to be Hb9-positive (Fig. 5A3). C57-iPS-12 ... See full document

... species to be used for scientific research, and has been widely applied as an animal model for studies in physiology, pharmacol- ogy, toxicology, nutrition, behavior, immunology, and neoplasia ...availability ... See full document

... IPS to a range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...effects of genetic ... See full document