Top PDF Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.
Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.
... RE19 from S7) carried fewer mutations, of which a subset was shared between them as well as with EPCs from the same ...None of the shared SNVs were detected in the corresponding fibroblasts or ...
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Physical passaging of embryoid bodies generated from human pluripotent stem cells.
... three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including human ...
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Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells.
... groups of cells to be the percentage of genes that are differentially expressed between them; thus, two ‘‘closer’’ groups will have a lower percentage of genes that are different ...
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Stimulation of GABA-induced Ca2+ influx enhances maturation of human induced pluripotent stem cell-derived neurons.
... U.K.). Cells plated on glass coverslips were incubated in β50µl of culture medium with a final concentration of 1µl of fura-β-AM (Life Technologies, Paisley, Strathclyde, ...re-emission ...
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Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
... The cells exhibited traditional human ES/iPS morphology with a high nuclear to cytoplasmic ratio ...5 cells we routinely used to start reprogramming, the number of human ...
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Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells.
... CD34+ cell population had hematopoietic and endothelial cell ...these cells were subjected to the hematopoietic colony-forming cell assay, various hematopoietic CFU colonies were ...
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Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts.
... rejection of syngeneic mouse iPSCs (miPSCs) following transplantation [17], in which miPSCs derived via the episomal approach were less prone to immune-mediated attack than those generated using viral ...
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Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
... Characterization of human induced pluripo- tent stem ...Primary human keratinocytes (PHK) derived from skin biopsy were reprogrammed to iPSC with a modified version ...
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DNA methylation dynamics in human induced pluripotent stem cells over time.
... Expression of tissue-specific genes, such as Oct-4 [4], Nanog [5], Sry (sex determining region on Y chromosome) [6] and MyoD [7], are induced by spatio-temporal demethylation during ...and cell ...
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Melanin Transfer in Human 3D Skin Equivalents Generated Exclusively from Induced Pluripotent Stem Cells.
... comprised of iPSC-derived fibroblasts, keratinocytes and melanocytes exhibited relatively normal tissue ...homed to the basal layer of the epidermis and produced melanin in this ...ratio of ...
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Scalable passaging of adherent human pluripotent stem cells.
... Advancing pluripotent stem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing Practices- ...
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Induced Pluripotent Stem Cells: Challenges and Opportunities
... capacity of mammals is limited and can rarely regenerate a specific organ or tissue ...Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of ...
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In Vitro Reconstruction of Neuronal Networks Derived from Human iPS Cells Using Microfabricated Devices.
... function of the nervous system is maintained via well-coordinated processes both in central and peripheral nervous tissues, which govern the homeostasis of organs/tis- ...Impairments of the nervous ...
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Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.
... Adult human dermal fibroblasts endogenously expressed Klf4 and c-Myc but not any other genes (data not ...iPS cell colony G also expressed Klf4 and c-Myc, indicating that these two genes were expressed ...
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Induced pluripotent stem cell modeling of Gaucher's disease: what have we learned?
... source of cells for ...original cells reprogrammed; this precludes the need for complex procedures to create the genetic defect and avoids potential secondary effects of the genetic ...
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The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
... Induced pluripotent stem cells (iPSCs) are derived from somatic cells by transfecting two pluripotent tran- scription factors, Oct4 (O) and Sox2 (S), and two proto- ...
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Expressão de marcadores de células germinativas e de oócitos em fibroblastos bovinos tratados com 5azacitidina e em célulastronco adultas cultivadas in vitro na presença de BMP2, BMP4 ou fluido folicular
... invulnerable to the passage of years, the impact of age on oocytes being consistent with its effect on the risk of congenital abnormalities (Balen, ...contain cells which can be ...
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Motoneuron differentiation of induced pluripotent stem cells from SOD1G93A mice.
... led to efficient motoneuron generation from iPS and E14 cell ...ALS-iPS-1 cell culture after 5 days treatment with RA and SHH ...found to be Hb9-positive (Fig. 5A3). C57-iPS-12 ...
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Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome.
... species to be used for scientific research, and has been widely applied as an animal model for studies in physiology, pharmacol- ogy, toxicology, nutrition, behavior, immunology, and neoplasia ...availability ...
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Genetic background drives transcriptional variation in human induced pluripotent stem cells.
... IPS to a range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...effects of genetic ...
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