[PDF] Top 20 sodC-based real-time PCR for detection of neisseria meningitidis
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sodC-based real-time PCR for detection of neisseria meningitidis
... Culture detection of Nm is 100% specific and should be attempted if possible, but it is limited by low sensitivity [31,32] and 24- to 72-hour incubation ...specificity of rt-PCR are rendering ... See full document
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Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
... spite of the recommendation to send frozen samples, bacterial DNA detection was not affected with transport of fresh CSF specimens at room temperature to the Reference ...advantage of ... See full document
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DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR ASSAYS FOR THE DETECTION OF NOSOCOMIAL PATHOGENS Deepa Anbazhagan
... Real time PCR is a technique used to monitor the progress of a PCR reaction in real ...same time, a relatively small amount of PCR product (DNA, cDNA or RNA) ... See full document
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Molecular diagnosis of Old World leishmaniasis: Real-time PCR based on tryparedoxin peroxidase gene for the detection and identification of Leishmania spp
... identification of Leishmania sp causing cutaneous leishmaniasis is crucial in control and therapeutic ...problem of diagnosis with traditional methods is that they have a low sensitivity or time ... See full document
4
Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.
... Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are common bacterial meningitis pathogens ...Traditional real-time PCR (rt-PCR) tests ... See full document
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Detection of bacterial 16S rRNA and identification of four clinically important bacteria by real-time PCR.
... sets of primers were tested for sensitivity and specificity for ...alignment of 54 ompA sequences, and the fifth primer, also targeting the ompA gene, has been published previously ...56uC, based on ... See full document
6
TaqMan MGB probe fluorescence real-time quantitative PCR for rapid detection of Chinese Sacbrood virus.
... region of the ...2. Detection of 37 Chinese sacbrood virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, ... See full document
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Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus
... conventional PCR based on direct visualization of amplicons (Castagnone et al ...analysis of amplicons (Burgermeister et al ...identification of the PWN. However, the multistep process ... See full document
7
A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)
... percent of nucleotide similarity among strains based on the vp60 gene (Table 2) and the nucleotide differences and substitutions between viral populations showed concordant values (Table ...total of ... See full document
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Detection of infectious laryngotracheitis virus by real-time PCR in naturally and experimentally infected chickens.
... fits of coughing before ...All of the specimens were homogenized, freeze-thawed three times, and treated with penicillin (500 IU/ml) and streptomycin (500 ...(CAMs) of 9-day-old ... See full document
10
Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen
... one of the most important infectious diseases (Poester et ...one of the most important infectious agents associated with this disease, which may also be due to Histophilus somni infection, as well as other ... See full document
5
Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens
... five of these samples were lower than the minimum detection threshold of the standard curve and these results were confirmed in a second assay with 40 cycles followed by melting curve ...six ... See full document
3
The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas
... copy of the partial housekeeping gene β-globin and TP53 from exon 7 were used as reference genes and co-amplified in a single tube reaction in the presence of the same DNA (Figure ...intensity of the ... See full document
6
In fluence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction
... no detection in low-concentration swabs (10 3 IFU/mL) after 40 days storage (all at ...type of swab used for sample collection, as some types of swabs have been shown to decrease the isolation rate ... See full document
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COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS
... variety of methods to detect N. meningitidis and, in some studies the methods were not even ...rate of approximately ...and real-time PCR, one was detected by culture alone, and ... See full document
5
Direct detection and differentiation of pathogenic Leptospira species using a multi-gene targeted real time PCR approach.
... direct detection of leptospiral infections in animals are in high demand, particularly to support suitable control ...presence of pathogenic viable leptospires in the kidneys or urine and ... See full document
11
A real-time PCR antibiogram for drug-resistant sepsis.
... multiplexed PCR [1,2], microarray platforms [3,4], and chemiluminescent RNA probes [5] for the genetic detection of known bacterial and fungal organisms responsible for ...handful of known ... See full document
6
Clinical evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Neisseria meningitidis in cerebrospinal fluid.
... Neisseria meningitidis (Nm) is a leading causative agent of bacterial meningitis in ...isolation of bacteria from patients’ cerebrospinal fluid (CSF) is time consuming and some- times ... See full document
13
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... the PCR-based detection of bacteria depends on the efficiency of DNA extraction procedure used to prepare the template ...strain of Listeria monocytogenes in 15 out of 24 ... See full document
9
Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... sensitivity of the qPCR assay development in this study was tested against a standard qPCR method- ology and the Ct values of the p3G primers were com- pared with PCR-primers targeting the 16S rDNA ... See full document
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