Top PDF In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.
In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.
... the differentiation of monkey ES cells into germ cells in vitro, we produced EBs and examined the expression of germ cell markers using RT-PCR (Figure ...
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The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
... transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no im- munological ...somatic cells, the ability of ntESCs to differenti- ate ...
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Stepwise development of hematopoietic stem cells from embryonic stem cells.
... role in initiation of primitive and definitive hematopoiesis ...arise from hemangioblasts ...EB6 cells instead of ...detected in the separated ...of cells arise from common ...
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Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
... manipulating stem cell differentiation is using feeder cell layers which are utilized in co-culture to mimic the effects of gonadal somatic cells and control PGC's ...
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Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells.
... bright cells derived from ...mouse embryonic fibroblasts (MEFs) supplemented with basic Fibroblast Growth Factor 2 (FGF2), Stem Cell Factor (SCF), Leukemia Inhibitory Factor (LIF) and ...
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Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts
... bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced ...murine embryonic fibroblasts ...layer. Cells were further ...
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Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
... GSC-like cells was not changed during in vitro differentiation ...GSC-like cells in the inner wells and testicular cells in the outer wells ...testicular ...
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Prion protein expression regulates embryonic stem cell pluripotency and differentiation.
... markers in the KO ESC, we examined the expression of bone morphogenetic protein (BMP) genes, which are related to the germ cell ...of germ cells, as the only cells in the ...
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Distinct roles for CBP and p300 on the RA-mediated expression of the meiosis commitment gene Stra8 in mouse embryonic stem cells.
... least in part, be functionally ...observed in knock-out mice indicate that CBP and p300 have unique functions [21], ...Although in vitro studies have demonstrated similar functions for CBP and ...
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Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
... like cell replacement therapy. Various cell sources have been used for cell transplantation, for example fetal and adult neural precursor cells ...these cells have a low proliferation ...
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Physical passaging of embryoid bodies generated from human pluripotent stem cells.
... three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including ...
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Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells
... Sertoli cells play a crucial role in the events of ...(stem cell factor (SCF), glial cell line- derivedneurotropic factor (GDNF), inhibin and FSH hormone) that are needed for ...
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Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo.
... Findings: In this work, we characterize the main stages and transitions that occur when ES cells are driven into a neural fate, using an adherent monolayer culture ...their embryonic counterparts, ...
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ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells.
... amplified from human cardiac precursor cell mRNA with specific primers and its DNA sequence was confirmed by ...protein in the bacterial host, we used the pDest17 expression vector system which is ...
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The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells
... AT-MSCs in vitro differentiation has demonstrated promising results (Zuk et ...for differentiation of mesenchymal stem cells (MSCs) into diverse cell lineages (Schäffler ...
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Expressão de marcadores de células germinativas e de oócitos em fibroblastos bovinos tratados com 5azacitidina e em célulastronco adultas cultivadas in vitro na presença de BMP2, BMP4 ou fluido folicular
... oocyte in a woman has the same age as ...contain cells which can be isolated and propagated, and have the characteristics of oogonial stem ...or in reaggregation models, these primitive ...
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Molecular mechanisms triggering differentiation of the embryonic endodermal stem cells
... of cells that carry the cloning vector-insert DNA contruct 73 . In this study, the cloning vector used was pGEM-T 2 (Promega GmbH, ...transformed cells that are grown in the presence of the ...
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Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
... Basic cell culture media, human serum albumin (HSA), trypsin, accutase, L-glutamine, and leukemia inhibitory factor (LIF) were purchased from PAA (Pasching, ...(stem cell factor (SCF), ...
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Design and Characterization of Bioengineered Cancer-Like Stem Cells.
... Cancer stem cells (CSCs) are a small subset of cancer cells responsible for maintenance and progression of several types of ...the differentiation of CSCs in the proper stem ...
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Reprogramming of Human Somatic Cells Using Human and Animal Oocytes
... nuclei from fresh human cumulus cells and then activated using ionomycin and 6-di- methylaminopurine ...four- cell (37 of 49) stage, whereas 19 (39%) of the SCNT embryos developed to the morula (8–16 ...
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