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Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).

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Academic year: 2017

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Table 1. A summary of seven organ types harvested at selective stages of plant development from six lines of narrow-leafed lupin, including four randomly selected F 8 recombinant inbred lines (RILs) and their parental lines, 83A:476 and P27255.
Table 2. Quantitative Reverse Transcription PCR (qRT-PCR) primers designed for seven candidate reference genes in narrow-leafed lupin (Lupi- (Lupi-nus angustifolius L.)
Table 3. A summary of the preliminary evaluation conducted for quantitative Reverse-Transcription PCR (qRT-PCR) primers of narrow-leafed lupin candidate reference genes.
Fig 1. Melt curve analyses of PDF2, UPL7, PTB, UBC, PPR, TUB and HEL candidate reference genes for quantitative Reverse-Transcription PCR (qRT-PCR) of narrow-leafed lupin.
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