The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure

The Comparison of Ef

_fi

ciency of Density Gradient

Centrifugation and Zeta Methods in Separation of

Mature Sperm with Normal Chromatin Structure

Mohammad Hossein Nasr Esfahani, Ph.D.1, 2_{*, Shahnaz Razavi, Ph.D.}3_{, Mohammad Mardani,}

Ph.D.3_{, Noush Afarin Khajavi, M.Sc.}3_{, Mohammad Reza Deemeh, M.Sc.}2_{, Marziyeh Tavalaee, M.Sc.}1

1. Reproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran

2. Isfahan Fertility and Infertility Center, Isfahan, Iran

3. Anatomy Department, Isfahan University of Medical Sciences, Isfahan, Iran

* Corresponding Address: P.O.Box: 19395-4644, Reproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran

Email: mh.nasr-esfahani@royaninstitute.org

Received: 16/Jun/2008, Accepted: 29/Sep/2008 Abstract

Objective: To examine the efficiency of both the Zeta and density gradient centrifugation (DGC) methods for the selection of normal chromatin sperm by TUNEL, sperm chromatin dispersion (SCD), acridine orange and chromomycin A3 (CMA3).

Materials and Methods: The study was performed on 63 patients. Semen analysis was car-ried out according to WHO criteria. Semen samples were divided into three equal portions. One portion was used as the control, the second portion was used for the Zeta method and the third portion underwent DGC method.

All portions were evaluated for sperm morphology (Diff Quick staining), protamine deficiency (CMA3) and DNA integrity (SCD, AO and TUNEL). Coefficients of correlation and students t test were carried out using SPSS 11.5.

Results: The mean percentage of abnormal sperm, protamine deficiency and DNA fragmen-tation in the Zeta and DGC methods were significantly decreased as compared to the control group (p<0.005). In addition, the DGC method was higher in comparison to the Zeta method in the selection of sperm with normal morphology (p<0.005). The Zeta method was higher in comparison to the DGC method in the selection of sperm with intact DNA (p<0.005).

Conclusion: Both Zeta and DGC methods were effective in the selection of sperm with better quality in terms of normal morphology, normal protamine content and DNA integrity. There-fore, we suggested that the combined Zeta and DGC method should be used for selection of normal sperm.

Keywords: Zeta Method, Protamine, Morphology, Sperm, DNA Fragmentation

ﺖﻬﺟ

Density Gradient Centrifugation

ﻭ

Zeta

ﺵﻭﺭ

ﻭﺩ

ﻰﻳﺍﺭﺎﻛ

ﻪﺴﻳﺎﻘﻣ

ﻰﻌﻴﺒﻃ

ﻦﻴﺗﺎﻣﻭﺮﻛ

ﺭﺎﺘﺧﺎﺳ

ﺎﺑ

ﻡﺮﭙﺳﺍ

ﻯﺯﺎﺳﺍﺪﺟ

،3

،،M.Sc. ﻯﻮﺟﺍﻮﺧﻦﻳﺮﻓﺁﺵﻮﻧ، 3

،،

Ph.D. ﻰﻧﺍﺩﺮﻣﺪﻤﺤﻣ،3 ،،

Ph.D. ﻯﻮﺿﺭﺯﺎﻨﻬﺷ،2 ،1

،،Ph.D. ﻰﻧﺎﻬﻔﺻﺍﺮﺼﻧﻦﻴﺴﺣﺪﻤﺤﻣ

1 ،،

M.Sc. ﻰﻳﻻﻮﺗﻪﻴﺿﺮﻣ،2

،،M.Sc. ﻪﻤﻳﺩﺎﺿﺭﺪﻤﺤﻣ

،ﻦﻳﻮﻜﺗﻭﻞﺜﻣﺪﻴﻟﻮﺗﻩﻭﺮﮔ،ﻰﻫﺎﮕﺸﻧﺍﺩﺩﺎﻬﺟﻰﻟﻮﻠﺳﻡﻮﻠﻋﺕﺎﻘﻴﻘﺤﺗﺰﻛﺮﻣ،ﻥﺎﻳﻭﺭﻩﺪﻜﺸﻫﻭﮋﭘ .1

ﻥﺍﺮﻳﺍ،ﻥﺎﻬﻔﺻﺍ

ﻥﺍﺮﻳﺍ،ﻥﺎﻬﻔﺻﺍ،ﻥﺎﻬﻔﺻﺍﻯﺭﻭﺭﺎﺑﺎﻧﻭﻯﺭﻭﺭﺎﺑﺰﻛﺮﻣ .2

ﻥﺍﺮﻳﺍ،ﻥﺎﻬﻔﺻﺍ،ﺢﻳﺮﺸﺗﻡﻮﻠﻋﻩﻭﺮﮔ،ﻥﺎﻬﻔﺻﺍﻰﻜﺷﺰﭘﻡﻮﻠﻋﻩﺎﮕﺸﻧﺍﺩ .3

،ﻰﻫﺎﮕﺸﻧﺍﺩﺩﺎﻬﺟﻰﻟﻮﻠﺳﻡﻮﻠﻋﺕﺎﻘﻴﻘﺤﺗﺰﻛﺮﻣ،ﻥﺎﻳﻭﺭﻩﺪﻜﺸﻫﻭﮋﭘ،19395-4644 :ﻲﺘﺴﭘﻕﻭﺪﻨﺻ،ﻥﺍﺮﻳﺍ،ﻥﺎﻬﻔﺻﺍ :ﻝﻮﺌﺴﻣﻩﺪﻨﺴﻳﻮﻧﺱﺭﺩﺁ *

ﻦﻳﻮﻜﺗﻭﻞﺜﻣﺪﻴﻟﻮﺗﻩﻭﺮﮔ

Email: mh.nasr-esfahani@royaninstitute.org:ﻚﻴﻧﻭﺮﺘﻜﻟﺍﺖﺴﭘ

87/7/7 :_{ﻪﻟﺎﻘﻣﺵﺮﻳﺬﭘ،}87/3/27 :_{ﻪﻟﺎﻘﻣﺖﻓﺎﻳﺭﺩ}

ﻩﺪﻴﻜﭼ

ﻯﺯﺎﺳﺍﺪﺟﺖﻬﺟ (DGC) Density Gradient CentrifugationﻭZetaﺵﻭﺭ ﻭﺩﻰﻳﺍﺭﺎﻛﻰﺑﺎﻳﺯﺭﺍ :ﻑﺪﻫ *

ﻡﺮﭙﺳﺍ ﻦﻴﺗﺎﻣﻭﺮﻛ ﻰﮔﺪﻨﻛﺍﺮﭘ ،TUNEL (AO) ﮋﻧﺍﺭﻭﺍ ﻦﻳﺪﻳﺮﻛﺁ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ ﻰﻌﻴﺒﻃ ﻦﻴﺗﺎﻣﻭﺮﻛ ﺭﺎﺘﺧﺎﺳ ﺎﺑ ﻡﺮﭙﺳﺍ .(Chromomycin A3; CMA3) A3 ﻦﻴﺴﻳﺎﻣﻮﻣﻭﺮﻛﻭ (Sperm Chromatin Dispersion; SCD) .ﺖﻓﺮﮔﺕﺭﻮﺻﻥﺎﻬﻔﺻﺍﻯﺭﻭﺭﺎﺑﺎﻧﻭﻯﺭﻭﺭﺎﺑﺰﻛﺮﻣﻪﺑﻩﺪﻨﻨﻛﻪﻌﺟﺍﺮﻣﺭﺎﻤﻴﺑ 63 ﻯﻭﺭﺮﺑﻪﻌﻟﺎﻄﻣﻦﻳﺍ _:ﺎﻫﺵﻭﺭﻭ ﺩﺍﻮﻣ _*

ﻯﺎﻫﻡﺮﭙﺳﺍ،ﻝﺮﺘﻨﻛ :ﺪﻳﺩﺮﮔﻢﻴﺴﻘﺗﺶﺨﺑﻪﺳﻪﺑﻦﻤﺳﻪﻧﻮﻤﻧﺲﭙﺳ .ﺪﺷﻡﺎﺠﻧﺍ_WHOﻯﺎﻫﺭﺎﻴﻌﻣﻖﺒﻃﻦﻤﺳﻊﻳﺎﻣﻦﻴﺗﻭﺭﺰﻴﻟﺎﻧﺁ

،ﺭﻮﻛﺬﻣﻯﺎﻫﻩﻭﺮﮔﺯﺍﻚﻳﺮﻫﻯﻭﺭﺮﺑﺲﭙﺳ .DGCﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﻭZetaﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻭ (CMA3ﻯﺰﻴﻣﺁﮓﻧﺭ) ﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ،(Diff Quickﻯﺰﻴﻣﺁﮓﻧﺭ) ﻡﺮﭙﺳﺍﻯژﻮﻟﻮﻓﺭﻮﻣﻰﺑﺎﻳﺯﺭﺍ

ﺭﺍﺰﻓﺍﻡﺮﻧﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑt testﻭﻲﮕﺘﺴﺒﻤﻫﺐﻳﺮﺿﻱﺭﺎﻣﺁﻱﺎﻫﻥﻮﻣﺯﺁﺎﺑﺞﻳﺎﺘﻧ .ﺖﻓﺮﮔﻡﺎﺠﻧﺍ (_AOﻭ _{TUNEL, SCD}) .ﺪﻧﺪﺷﻞﻴﻠﺤﺗﻭﻲﺳﺭﺮﺑSPSS-11/5

ﻩﻭﺮﮔﻭﺩﺭﺩDNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺪﺻﺭﺩﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛ،ﻰﻌﻴﺒﻃﺮﻴﻏﻯژﻮﻟﻮﻓﺭﻮﻣﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩ :ﺎﻫﻪﺘﻓﺎﻳ *

ﺖﺳﺍﻪﺘﻓﺎﻳﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛﻯﺭﺎﻣﺁﻅﺎﺤﻟﺯﺍﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑﺖﺒﺴﻧDGCﻭZetaﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍ

ﺖﺳﺍﻩﺩﻮﻤﻧﻞﻤﻋﺮﺘﻬﺑZetaﺵﻭﺭﻪﺑﺖﺒﺴﻧﻰﻌﻴﺒﻃﻯژﻮﻟﻮﻓﺭﻮﻣﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺭﺩDGCﺵﻭﺭﻩﻭﻼﻋﻪﺑ .(p_<0/005)

ﺮﺘﻬﺑDGCﺵﻭﺭﻪﺑﺖﺒﺴﻧﺮﺘﻤﻛDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺭﺩZetaﺵﻭﺭﻪﻛﻰﻟﺎﺣﺭﺩ (_p<0/005) .(p<0/005) ﺖﺳﺍﻩﺩﻮﻤﻧﻞﻤﻋ

ﻭ ﻯژﻮﻟﻮﻓﺭﻮﻣﺎﺑ ﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟ ﻪﺑﺭﺩﺎﻗ ﻯﺭﺍﺩﻰﻨﻌﻣ ﺭﻮﻃ ﻪﺑDGC ﻭ Zeta ﺵﻭﺭﻭﺩ ﺮﻫ _:ﻱﺮﻴﮔﻪﺠﻴﺘﻧ _*

ﻲﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍﻱﺯﺎﺳﺍﺪﺟﻱﺍﺮﺑZetaﻭDGCﺵﻭﺭﻭﺩﺮﻫﺯﺍﺩﺩﺮﮔﻲﻣﺩﺎﻬﻨﺸﻴﭘﻦﻳﺍﺮﺑﺎﻨﺑ .ﺪﺷﺎﺑﻰﻣﻰﻌﻴﺒﻃﻦﻴﺗﺎﻣﻭﺮﻛﺭﺎﺘﺧﺎﺳ .ﺩﻮﺷﻩﺩﺎﻔﺘﺳﺍ

DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ،ﻡﺮﭙﺳﺍ،ﻱژﻮﻟﻮﻓﺭﻮﻣ،ﻦﻴﻣﺎﺗﻭﺮﭘ،ﺎﺗﺯﺵﻭﺭ :ﻥﺎﮔژﺍﻭﺪﻴﻠﻛ *

168-175 :ﺕﺎﺤﻔﺻ،88 ﻥﺎﺘﺴﺑﺎﺗ،2 ﻩﺭﺎﻤﺷ،ﻢﻫﺩﺯﺎﻳﻝﺎﺳ،ﻪﺘﺧﺎﻳﻰﻜﺷﺰﭘﻪﻣﺎﻨﻠﺼﻓ

ﻪﻣﺪﻘﻣ

ﻚﻤﺨﺗ ﻢﺳﻼﭘﻮﺘﻴﺳ ﻞﺧﺍﺩ ﻪﺑ ﻡﺮﭙﺳﺍ ﻖﻳﺭﺰﺗ ﻚﻴﻨﻜﺗ ﻩﺯﻭﺮﻣﺍ

ﻚﻳ ﻥﺍﻮﻨﻋ ﻪﺑ (Intra Cytoplasmic Sperm Injection; ICSI)

ﺭﺩ ﻪﻧﺍﺩﺮﻣ ﻯﺎﻫﺭﻮﺘﻛﺎﻓﺖﻠﻋ ﻪﺑ ﻯﺭﻭﺭﺎﺑﺎﻧﻥﺎﻣﺭﺩﺖﻬﺟﺐﺳﺎﻨﻣﺵﻭﺭ ﺎﺸﮕﻫﺍﺭ (In Vitro _{Fertilization)}ﻭIVFﺵﻭﺭﺎﻬﻧﺁ ﺭﺩﻪﻛﻰﻧﺍﺭﺎﻤﻴﺑ

،ICSI ﻡﺎﺠﻧﺍﻡﺎﮕﻨﻫﺭﺩﻪﻛﻰﻳﺎﺠﻧﺁﺯﺍ .(1) ﺩﻭﺭﻰﻣﺭﺎﻛﻪﺑ،ﺪﺷﺎﺑﻰﻤﻧ

ﻯژﻮﻟﻮﻓﺭﻮﻣﻯﺍﺭﺍﺩﻪﻛﺩﻮﺷﻲﻣﺏﺎﺨﺘﻧﺍﺱﺎﻨﺷﻦﻴﻨﺟﻂﺳﻮﺗﻰﻣﺮﭙﺳﺍﺎﻬﻨﺗ ﺖﻳﻭﺭﻞﺑﺎﻗﻥﺎﻧﺁﻂﺳﻮﺗﻡﺮﭙﺳﺍﻪﺘﺴﻫﺖﻣﻼﺳﻭﺪﺷﺎﺑﻰﻌﻴﺒﻃﺖﻛﺮﺣﻭ ﻪﺑ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩﻡﺮﭙﺳﺍﻖﻳﺭﺰﺗﻥﺎﻜﻣﺍﻦﻳﺍﺮﺑﺎﻨﺑ .ﺪﺷﺎﺑﻰﻤﻧ

ﻩﺩﻮﻤﻧ ﺩﺎﺠﻳﺍﻚﻴﻨﻜﺗﻦﻳﺍﻡﺎﺠﻧﺍﺖﻬﺟﺍﺭﻰﻳﺎﻫﻰﻧﺍﺮﮕﻧﻚﻤﺨﺗ ﻞﺧﺍﺩ ﻥﺍﺰﻴﻣﻞﻗﺍﺪﺣﻯﺍﺭﺍﺩﻭﻎﻟﺎﺑﻭﻰﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍﺏﺎﺨﺘﻧﺍﺍﺬﻟ .(2) ﺖﺳﺍ

ﺎﺻﻮﺼﺧﻯﺭﻭﺭﺎﺑﻚﻤﻛﻯﺎﻫﻚﻴﻨﻜﺗﻰﻣﺎﻤﺗﺭﺩ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

.(3) ﺪﺳﺭﻰﻣﺮﻈﻧﻪﺑﻯﺭﻭﺮﺿICSI

ﻰﻌﻴﺒﻃﻭﻎﻟﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﻯﺍﺮﺑﻰﻋﻮﻨﺘﻣﻭﺪﻳﺪﺟﻯﺎﻫﺵﻭﺭ ،ﻎﻟﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺎﻬﻧﺁﻰﻣﺎﻤﺗﻑﺪﻫﻪﻛﺪﻧﺍﻩﺪﺷﻰﻓﺮﻌﻣﻥﻮﻨﻛﺎﺗ ﻪﺑﻥﺍﻮﺗﻰﻣﺎﻫﺵﻭﺭﻦﻳﺍﻪﻠﻤﺟﺯﺍ .ﺪﺷﺎﺑﻰﻣﻙﺮﺤﺗﻭﻯژﻮﻟﻮﻓﺭﻮﻣﻅﺎﺤﻟﺯﺍ

.(4) ﺩﻮﻤﻧﻩﺭﺎﺷﺍ (DGC) Density Gradient Centrifugation ﺵﻭﺭ

ﻩﺩﻭﺪﺤﻣﺭﺩﻙﺮﺤﺗﻭﻯژﻮﻟﻮﻓﺭﻮﻣﻅﺎﺤﻟﺯﺍﻪﻛﻰﻳﺎﻫﻡﺮﭙﺳﺍﺵﻭﺭﻦﻳﺍﺭﺩ ﺍﺪﺟﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍﺯﺍﻰﺘﻴﺴﻧﺍﺩﻑﻼﺘﺧﺍﺱﺎﺳﺍﺮﺑﺪﻧﺭﺍﺩﺭﺍﺮﻗﻰﻌﻴﺒﻃ ﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩﺪﻧﺍﻩﺩﺍﺩﻥﺎﺸﻧﻦﻳﺍﺯﺍﺶﻴﭘﻥﻮﮔﺎﻧﻮﮔﺕﺎﻌﻟﺎﻄﻣﻪﭽﻧﺎﻨﭼ .ﺪﻧﻮﺷﻰﻣ

ﻯﺎﻫﻡﺮﭙﺳﺍژﻮﻴﻔﻳﺮﺘﻧﺎﺳﻦﻴﺣﺭﺩﺍﺬﻟ .ﺪﺷﺎﺑﻰﻣﺮﺘﺸﻴﺑﻢﺠﺣﻪﺑﻥﺯﻭﺖﺒﺴﻧﻰﻌﻴﺒﻃ

،ﺪﻧﺭﺍﺩﻯﺮﺘﺸﻴﺑﻰﻟﺎﮕﭼﻭﻩﺩﻮﺑﺮﺗﻢﻛﺍﺮﺘﻣﻪﻛﻰﻌﻴﺒﻃﻦﻴﺗﺎﻣﻭﺮﻛﺭﺎﺘﺧﺎﺳﻯﺍﺭﺍﺩ

.(6 ،5) ﺪﻧﻮﺷﻰﻣﻦﻴﺸﻧﻪﺗﺮﺗﻊﻳﺮﺳ

ﺩﺍﻮﻣ ﻯﺮﻴﮔﺭﺎﻛﻪﺑ ﻅﺎﺤﻟ ﺯﺍ DGC ﺵﻭﺭ ﻯﺎﻫﺖﻳﺩﻭﺪﺤﻣ ﻞﻴﻟﺩ ﻪﺑ

ﺯﺭﻮﻓﻭﺮﺘﻜﻟﺍﺵﻭﺭﻭﺩ .ﺩﻮﻤﻧﻩﺭﺎﺷﺍﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﺱﺎﺳﺍﺮﺑﻰﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍ

ﺮﺑﺩﻮﺟﻮﻣﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﺱﺎﺳﺍﺮﺑﺍﺭﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﻥﺎﻜﻣﺍZetaﻭ

ﺶﻧﺍﺭﺎﻜﻤﻫﻭﻥﺎﭼﻂﺳﻮﺗﻪﻛZetaﺵﻭﺭﺭﺩ .ﺪﻳﺎﻤﻧﻰﻣﻢﻫﺍﺮﻓﺎﺸﻏﺢﻄﺳ Zetaﻞﻴﺴﻧﺎﺘﭘﺱﺎﺳﺍﺮﺑﻰﻌﻴﺒﻃﻡﺮﭙﺳﺍﺏﺎﺨﺘﻧﺍ،ﺪﺷﺩﺎﻬﻨﺸﻴﭘ 2006 ﻝﺎﺳﺭﺩ

ﺭﺍﺩﺭﺎﺑﻱﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﻮﻟﺎﻴﺳﻪﺑﻪﺘﺴﺑﺍﻭZetaﻞﻴﺴﻧﺎﺘﭘ .ﺩﺮﻴﮔﻰﻣﺕﺭﻮﺻ

ﺩﻮﺷﻲﻣﻩﺩﻭﺰﻓﺍﻥﺁﺢﻄﺳﻪﺑ،ﻡﺮﭙﺳﺍﻍﻮﻠﺑﺪﻨﻳﺍﺮﻓﻰﻃﺭﺩﻪﻛﺖﺳﺍﻡﺮﭙﺳﺍﺎﺸﻏ

.(8 ،7)

ﻱﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔ ﺯﺍ ﻰﺧﺮﺑ ﻪﻛ ﺪﻫﺩﻰﻣ ﻥﺎﺸﻧ ﺮﻴﺧﺍ ﺕﺎﻘﻴﻘﺤﺗ ﻲﻠﺳﺎﻨﺗ ﻩﺎﮕﺘﺳﺩ ﺭﺩ ﻥﺁ ﺖﻇﺎﻔﺣ ﻱﺍﺮﺑ ﻡﺮﭙﺳﺍ ﺢﻄﺳ ﻪﺑ ﻩﺪﺷ ﻩﺩﻭﺰﻓﺍ

ﺡﻼﻄﺻﺍ ﺭﺩ ﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔ ﻦﻳﺍ ﻪﺑ ﻭ ﺪﺷﺎﺑﻲﻣ ﻯﺭﻭﺮﺿ ﺚﻧﻮﻣ

ﻝﺍﺮﮕﺘﻨﻳﺍﻯﺎﻫﻦﻴﺌﺗﻭﺮﭘﻪﻠﻤﺟﺯﺍ .ﺩﻮﺷﻲﻣﻪﺘﻔﮔ Maturation Antigen

ﻪﻛﺩﻮﻤﻧﻩﺭﺎﺷﺍGp20ﻪﺑ ﻥﺍﻮﺗﻰﻣﻡﺮﭙﺳﺍﻯﺎﺸﻏﺢﻄﺳﻪﺑ ﻩﺪﺷﻪﻓﺎﺿﺍ

ﻥژﻲﺘﻧﺁﺎﺑگﻮﻟﻮﻤﻫﻭﺪﺷﺎﺑﻲﻣﺎﺸﻏﻰﺤﻄﺳﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﻮﻟﺎﻴﺳﻚﻳ ﻯﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﺯﺍ ﻰﻜﻳ Gp20 .ﺖﺳﺍ ﺖﻴﺳﻮﻜﻟ ﺎﺸﻏﺭﺩ CD52

ﻩﺎﮕﺘﺳﺩﺯﺍﻩﺪﺷ ﻝﺍﺰﻧﺍﻡﺮﭙﺳﺍﻯﺎﺸﻏﻰﻔﻨﻣﻲﻜﻳﺮﺘﻜﻟﺍ ﺭﺎﺑﺩﺎﺠﻳﺍ ﻝﻮﺌﺴﻣ

.(9) ﺪﺷﺎﺑﻰﻣﺮﻛﺬﻣﻰﻠﺳﺎﻨﺗ

ﺭﺎﺘﺧﺎﺳ ﺭﺩ ﺮﻴﻴﻐﺗ ﻪﻛ ﺪﻫﺩﻰﻣ ﻥﺎﺸﻧ ﻥﻮﮔﺎﻧﻮﮔ ﻯﺎﻫﺶﻫﻭﮋﭘ

ﻥﺪﺷ ﻞﻳﻮﻃ ،ﻡﻭﺯﻭﺮﻛﺁ ﻞﻴﻜﺸﺗ ،ﻲﻳﺎﻤﺳﻼﭘ ﺎﺸﻏ ﻱﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔ

ﺪﻨﻳﺍﺮﻓﺭﺩﻪﻛﺖﺳﺍﻰﺗﺍﺮﻴﻴﻐﺗﻪﻠﻤﺟﺯﺍﻦﻴﺗﺎﻣﻭﺮﻛﺭﺎﺘﺧﺎﺳﺭﺩﺮﻴﻴﻐﺗﻭﻡﺩ ﺭﺎﺘﺧﺎﺳ ﻢﻛﺍﺮﺗ ﺪﻨﻳﺍﺮﻓ ﺭﺩ ﻪﭽﻧﺎﻨﭼ .(10-12) ﺪﻫﺩﻰﻣ ﺥﺭ ﺰﻧژﻮﻴﻣﺮﭙﺳﺍ

ﺽﺮﻌﻣﺭﺩﻡﺮﭙﺳﺍDNA،ﺩﻮﺷﺩﺎﺠﻳﺍﻰﻟﻼﺘﺧﺍﻪﻧﻮﮔﺮﻫ،ﻪﺘﺴﻫﻦﻴﺗﺎﻣﻭﺮﻛ

ﻥﺍﺩﺮﻣﻡﺮﭙﺳﺍﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧﻒﻠﺘﺨﻣﺕﺎﻌﻟﺎﻄﻣ .(13) ﺩﺮﻴﮔﻰﻣﺭﺍﺮﻗﺐﻴﺳﺁ

ﺖﺳﺍﻪﺘﺷﺍﺩﺭﻭﺭﺎﺑﻥﺍﺩﺮﻣﺎﺑﻪﺴﻳﺎﻘﻣﺭﺩﻯﺮﺗﻻﺎﺑ DNAﺐﻴﺳﺁﻥﺍﺰﻴﻣﺭﻭﺭﺎﺑﺎﻧ

ﺖﺒﺴﻧﺖﻣﻭﺎﻘﻣﻥﺍﺰﻴﻣ،ﺪﻧﺩﺮﻛﻥﺎﻴﺑﺶﻧﺍﺭﺎﻜﻤﻫﻭﻦﻳﻮﻳﺭﺁﻦﻴﻨﭼﻢﻫ .(14-16)

ﻯﺩﺎﻳﺯﻥﺍﺰﻴﻣﻪﺑﺭﻭﺭﺎﺑﺎﻧﺩﺍﺮﻓﺍﺭﺩﺰﻴﻧﺪﻴﺳﺍﻯﺎﻘﻟﺍﺖﺤﺗDNAﻥﺪﺷﻩﺭﻮﺗﺎﻧﺩﻪﺑ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ،ﺕﺎﻘﻴﻘﺤﺗﺯﺍﻩﺪﻣﺁﺖﺳﺩﻪﺑﺞﻳﺎﺘﻧﺱﺎﺳﺍﺮﺑ .(17) ﺪﺑﺎﻳﻰﻣﺶﻫﺎﻛ

ﺪﻧﺍﻮﺗﻰﻣﻦﻳﺍﺮﺑﺎﻨﺑ،ﺖﺳﺍﻪﺘﺷﺍﺩﻯﺮﻳﺬﭘﺎﻧﺭﺎﻜﻧﺍﻁﺎﺒﺗﺭﺍﻯﺭﻭﺭﺎﺑﻥﺍﺰﻴﻣﺎﺑDNA

ﻥﺍﺩﺮﻣﺭﺩﻯﺭﻭﺭﺎﺑﺕﺭﺪﻗﻰﻳﻮﮔﺶﻴﭘﺖﻬﺟﻞﻘﺘﺴﻣﺭﻮﺘﻛﺎﻓﻚﻳﻥﺍﻮﻨﻋﻪﺑ

DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻥﺪﻣﺁﺩﻮﺟﻭﻪﺑﺚﻋﺎﺑﻪﻛﻰﻠﻣﺍﻮﻋﻪﻠﻤﺟﺯﺍ .(15) ﺪﺷﺎﺑ

ﻰﻨﻳﺰﮕﻳﺎﺟﺭﺩﻝﻼﺘﺧﺍﻭﺯﻮﺘﭘﻮﭘﺁ،ﻮﻴﺗﺍﺪﻴﺴﻛﺍﻯﺎﻫﺱﺮﺘﺳﺍﻪﺑﻥﺍﻮﺗﻰﻣ،ﺩﻮﺷﻰﻣ

.(18 ،13-15) ﺩﺮﻛﻩﺭﺎﺷﺍﻦﻴﻣﺎﺗﻭﺮﭘﻪﻠﻴﺳﻭﻪﺑﺎﻫﻥﻮﺘﺴﻴﻫ

ﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﻰﻳﺎﺳﺎﻨﺷﺖﻬﺟ A3ﻦﻴﺴﻳﺎﻣﻮﻣﻭﺮﻛﻯﺰﻴﻣﺁﮓﻧﺭ

ﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧﻒﻠﺘﺨﻣﺕﺎﻌﻟﺎﻄﻣ .(19) ﺩﻭﺭﻰﻣﺭﺎﻛﻪﺑﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛ

ﻰﻌﻴﺒﻃﺩﺍﺮﻓﺍﻪﺑﺖﺒﺴﻧﻯﺮﺘﻤﻛﻦﻴﻣﺎﺗﻭﺮﭘﻥﺍﺰﻴﻣﻯﺍﺭﺍﺩﺭﻭﺭﺎﺑﺎﻧﻥﺍﺩﺮﻣﻡﺮﭙﺳﺍ

.(21 ،20) ﺪﺷﺎﺑﻰﻣ

ﻯﺩﺪﻌﺘﻣ ﻯﺎﻫﺵﻭﺭ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻥﺍﺰﻴﻣ ﻰﺳﺭﺮﺑ ﺖﻬﺟ

،(14) TUNEL ﺵﻭﺭ ﻪﺑ ﻥﺍﻮﺗﻰﻣ ﺎﻬﻧﺁ ﻦﻳﺮﺗﻢﻬﻣﺯﺍ ﻪﻛ ﺩﺭﺍﺩﺩﻮﺟﻭ

،(16) Sperm Chromatin Structure Assay (SCSA)A

ﻭ (22) Sperm Chromatin Dispersion (SCD)

.ﺩﻮﻤﻧﻩﺭﺎﺷﺍ (23) Acridin Orange Staining (AO)

ﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭﻭﺩﻡﺎﺠﻧﺍﺯﺍﺲﭘﺪﺷﻪﺘﻓﺮﮔﻢﻴﻤﺼﺗﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩ ﻦﻴﺗﺎﻣﻭﺮﻛﺭﺎﺘﺧﺎﺳ،ﺭﻭﺭﺎﺑﺎﻧﺩﺍﺮﻓﺍﻦﻤﺳﻪﻧﻮﻤﻧﻯﻭﺭﺮﺑZeta ﻭDGCﻡﺮﭙﺳﺍ CMA3ﻭAOﻭ SCDﻭTUNELﻚﻴﻨﻜﺗﺭﺎﻬﭼﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑﻡﺮﭙﺳﺍ

ﺮﻫﺭﺩﻰﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻦﻴﻴﻌﺗﺯﺍﺲﭘﺎﺗﺩﺮﻴﮔﺭﺍﺮﻗﻰﺳﺭﺮﺑﺩﺭﻮﻣ

.ﺩﺩﺮﮔﺺﺨﺸﻣﺵﻭﺭﻭﺩﻦﻳﺍﻰﻳﺍﺭﺎﻛﻥﺍﺰﻴﻣﻩﻭﺮﮔ

ﺎﻫ

ﺵﻭﺭ

ﻭ

ﺩﺍﻮﻣ

ﻲﺳﺭﺮﺑﺵﻭﺭ

63 ﻦﻤﺳﻪﻧﻮﻤﻧﺯﺍﻭﻩﺩﻮﺑﻩﺩﺎﺳﺵﻭﺭﻪﺑﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩﻪﻧﻮﻤﻧﺏﺎﺨﺘﻧﺍ .ﺪﺷﻩﺩﺎﻔﺘﺳﺍﻥﺎﻬﻔﺻﺍﻯﺭﻭﺭﺎﺑﺎﻧﻭﻯﺭﻭﺭﺎﺑﺰﻛﺮﻣﻪﺑﻩﺪﻨﻨﻛﻪﻌﺟﺍﺮﻣﺭﻭﺭﺎﺑﺎﻧﺩﺮﻓ

ﻯﺭﺍﺩﺩﻮﺧﺯﻭﺭ 3-4 ﺯﺍﺪﻌﺑ،ﻦﻤﺳﻱﺎﻫﻪﻧﻮﻤﻧﻭﺬﺧﺍﺖﻳﺎﺿﺭﻡﺮﻓﻥﺍﺭﺎﻤﻴﺑﺯﺍ

ﻙﺮﺤﺗ،ﺖﻈﻠﻏ )ﻦﻤﺳﻊﻳﺎﻣﻦﻴﺗﻭﺭﺰﻴﻟﺎﻧﺁﻭﻯﺭﻭﺁﻊﻤﺟ،ﺖﺑﺭﺎﻘﻣﺯﺍﻦﻴﺟﻭﺯ

ﻡﺎﺠﻧﺍﻯﺭﻮﻧپﻮﻜﺳﻭﺮﻜﻴﻣﻂﺳﻮﺗ،(24) WHO ﺭﺎﻴﻌﻣﻖﺒﻃ (ﻯژﻮﻟﻮﻓﺭﻮﻣﻭ

ﺩﺭﺍﻮﻣﺰﺟﻪﺑﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩﻰﻓﺮﺼﻣﺩﺍﻮﻣﻰﻣﺎﻤﺗﺖﺳﺍﺮﻛﺫﻪﺑﻞﺑﺎﻗ .ﺪﻳﺩﺮﮔ

ﻪﺑﻮﺼﻣﺡﺮﻃﻦﻳﺍ .ﺖﺳﺍﻩﺪﻳﺩﺮﮔﻪﻴﻬﺗﺎﻤﮕﻴﺳﺖﻛﺮﺷﺯﺍﻦﺘﻣﺭﺩﻩﺪﺷﻩﺭﺎﺷﺍ .ﺖﺳﺍﻥﺎﻳﻭﺭﻩﺪﻜﺸﻫﻭﮋﭘ

ﻡﺮﭙﺳﺍﻯﺯﺎﺳﻩﺩﺎﻣﺁ

2000ﺭﻭﺩ ﺎﺑﻪﻘﻴﻗﺩ ﻩﺩ ﺕﺪﻣﻪﺑ ﻯﺭﻭﺁﻊﻤﺟﺯﺍﺲﭘ ﺍﺭﻦﻤﺳﻊﻳﺎﻣ

،ﻪﻴﻠﺨﺗﻲﻳﻭﺭﻊﻳﺎﻣ .ﺩﻮﺷﻞﻴﻜﺸﺗﺎﻫﻡﺮﭙﺳﺍﺯﺍﻰﺑﻮﺳﺭﺎﺗﻩﺩﻮﻤﻧژﻮﻴﻔﻳﺮﺘﻧﺎﺳ

ﺯﺍﺲﭘﻭﻪﺘﺨﻳﺭﺏﻮﺳﺭﻱﻭﺭHam’s+FCSﺪﺻﺭﺩ 10 ﻂﻴﺤﻣﺲﭙﺳ

ﻪﻴﻠﺨﺗﺍﺭﻲﻳﻭﺭﻊﻳﺎﻣﻭژﻮﻴﻔﻳﺮﺘﻧﺎﺳﻪﻘﻴﻗﺩ 5 ﺕﺪﻣﻪﺑﺍﺭﻝﻮﻠﺤﻣ،ﻥﺩﻮﻤﻧﺖﭙﻴﭘ

ﻪﺳﻪﺑﻥﺍﺭﺎﻤﻴﺑﻦﻳﺍﻦﻤﺳﻪﻧﻮﻤﻧ .ﺪﻳﺩﺮﮔﻪﻓﺎﺿﺍﺏﻮﺳﺭﻪﺑHam’sﻂﻴﺤﻣﻭ

ﺮﮕﻳﺩﺶﺨﺑﻭﺩﻭﻝﺮﺘﻨﻛﻩﻭﺮﮔﻥﺍﻮﻨﻋﻪﺑﺶﺨﺑﻚﻳﻪﻛﺪﺷﻢﻴﺴﻘﺗﺶﺨﺑ ﻩﺩﺎﻔﺘﺳﺍﺩﺭﻮﻣZetaﻭDGCﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭﻭﺩﻪﺴﻳﺎﻘﻣﺖﻬﺟ

ﻰﺑﺎﻳﺯﺭﺍ،ﻪﻧﺎﮔﺍﺪﺟﺭﻮﻃﻪﺑﺎﻫﻩﻭﺮﮔﺯﺍﻡﺍﺪﻛﺮﻫﻯﻭﺭﺮﺑﺲﭙﺳ .ﺖﻓﺮﮔﺭﺍﺮﻗ

ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛ،(25) Diff Quickﻱﺰﻴﻣﺁﮓﻧﺭﻂﺳﻮﺗﻡﺮﭙﺳﺍﻱژﻮﻟﻮﻓﺭﻮﻣ TUNEL ،SCD ﺖﺴﺗﻪﺳﻭ (19) A3ﻦﻴﺴﻳﺎﻣﻮﻣﻭﺮﻛﻱﺰﻴﻣﺁﮓﻧﺭﻂﺳﻮﺗ

ﺩﺭﻮﻣDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻥﺍﺰﻴﻣﻦﻴﻴﻌﺗﺖﻬﺟ Acridine Orangeﻭ

.(23 ،22 ،14) ﺖﻓﺮﮔﺭﺍﺮﻗﻰﺑﺎﻳﺯﺭﺍ

ﺖﻈﻠﻏﺐﻴﺷژﻮﻴﻔﻳﺮﺘﻧﺎﺳﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭ

(Density Gradient Centrifugation; DGC)

ﺪﺻﺭﺩ 80 Pure Spermﻝﻮﻠﺤﻣﺯﺍﻯﺍ ﻪﻳﻻﻭﺩ ﻥﺎﻳﺩﺍﺮﮔﻚﻳ

ﻥﻮﺘﺳﻚﻳﺎﺗﺩﺩﺮﮔﻰﻣﻪﻴﻬﺗﻲﺳﻲﺳ 10 ﻥﻮﻜﻟﺎﻓﻪﻟﻮﻟﺭﺩﺪﺻﺭﺩ 40 ﻭ

.ﺩﻮﺷﺩﺎﺠﻳﺍﺍﺰﺠﻣﺖﻈﻠﻏﺐﻴﺷﻭﺩﺎﺑﻪﻳﻻﻭﺩ Pure Spermﺯﺍﺪﺘﻤﻣ

ﺭﺩﻪﻘﻴﻗﺩ 20 ﺕﺪﻣﻪﺑﻭﻪﺘﺨﻳﺭﻥﺁﻯﻭﺭﻦﻤﺳﻊﻳﺎﻣﺯﺍﺮﺘﻴﻟﻰﻠﻴﻣ 1/5 ﺲﭙﺳ DGCﺵﻭﺭﻡﺎﺠﻧﺍﺖﻬﺟ،ﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩ .ﺩﻮﺷﻰﻣژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺭﻭﺩ 2000 .ﺪﻳﺩﺮﮔﻩﺩﺎﻔﺘﺳﺍ (ﺪﺋﻮﺳﻩﺪﻧﺯﺎﺳﺭﻮﺸﻛ) _{Pure sperm}ﻯﺭﺎﺠﺗﻩﺩﺎﻣﺯﺍ

ﻩﺮﻴﭙﺳﺁﺭﻮﺘﺳﺎﭘﺖﭙﻴﭘﺎﺑﻪﻟﻮﻟﻯﻻﺎﺑﺭﺩﺩﻮﺟﻮﻣﻊﻳﺎﻣ،ژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺯﺍﺲﭘ ﻞﻘﺘﻨﻣﻲﺳﻲﺳ 5 ﻥﻮﻜﻟﺎﻓﻪﻟﻮﻟﻚﻳﻪﺑﻪﻟﻮﻟﺕﺎﻳﻮﺘﺤﻣﻩﺪﻧﺎﻣﻰﻗﺎﺑﻭﻩﺪﺷ

ﻥﻮﻴﺴﻧﺎﭙﺳﻮﺳﺲﭙﺳ .ﺪﺷﻁﻮﻠﺨﻣ،Sperm Rinseﻂﻴﺤﻣﺎﺑﻭﻩﺪﻳﺩﺮﮔ

ﺍﺩﺪﺠﻣ .ﺪﻳﺩﺮﮔژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺭﻭﺩ 2000 ﺭﺩﻪﻘﻴﻗﺩ 10 ﺕﺪﻣﻪﺑﻩﺪﺷﺩﺎﺠﻳﺍ

ﺭﺩ ﻩﺪﺷﻞﻴﻜﺸﺗﺖﻴﻠﭘﻭﻩﺪﺷ ﻪﺘﺷﺍﺩﺮﺑﻰﻳﻭﺭﻊﻳﺎﻣ،ژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺯﺍﺲﭘ ﻡﺎﺠﻧﺍ ﺖﻬﺟﺖﺳﺍﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟ ﻯﺎﻫﻡﺮﭙﺳﺍ ﻯﻮﺘﺤﻣﻪﻛﻪﻟﻮﻟﻯﺎﻬﺘﻧﺍ

.(4) ﺪﻳﺩﺮﮔﻩﺩﺎﻔﺘﺳﺍICSI

Zetaﺵﻭﺭ

ﺭﺎﺑﻭﺩﺯﺍﺲﭘﻭﻪﺘﺨﻳﺭژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺹﻮﺼﺨﻣﻪﻟﻮﻟﺭﺩﻦﻤﺳﻪﻧﻮﻤﻧﺍﺪﺘﺑﺍ ﻥﻭﺮﻴﺑﻭﻩﺪﺷﻩﺪﻧﺎﺧﺮﭼﺲﻜﺗﻻﺶﻜﺘﺳﺩﻥﻭﺭﺩﻪﺒﺗﺮﻣﻪﺳﻊﻳﺮﺳ،ﻮﺷﻭﺖﺴﺷ ﻪﻘﻴﻗﺩﻚﻳﺕﺪﻣﻪﺑﻪﻧﻮﻤﻧﻱﻭﺎﺣﺭﺍﺩﺭﺎﺑﻪﻟﻮﻟ .ﺩﺩﺮﮔﺭﺍﺩﺭﺎﺑﺎﺗﺩﻮﺷﻰﻣﻩﺪﻴﺸﻛ

ﻱﺍﺭﺍﺩﻎﻟﺎﺑﻱﺎﻫﻡﺮﭙﺳﺍﺎﺗﺩﻮﺷﻰﻣﻪﺘﺷﺍﺬﮔ (ﻪﺟﺭﺩ 22) ﻩﺎﮕﺸﻳﺎﻣﺯﺁﻯﺎﻣﺩﺭﺩ

ﻩﺪﺷژﻮﻴﻔﻳﺮﺘﻧﺎﺳﻪﻘﻴﻗﺩ 5 ﺕﺪﻣﻪﺑﻕﻮﻓﻪﻟﻮﻟ .ﺪﻨﺒﺴﭽﺑﻪﻟﻮﻟﺭﺍﺪﺟﻪﺑﻲﻔﻨﻣﺭﺎﺑ

ﻪﺑﺭﻮﺘﺳﺎﭘﺖﭙﻴﭘﺎﺑﺶﻧﻭﺭﺩﻱﺎﻫﻡﺮﭙﺳﺍﻝﻮﻠﺤﻣﻭﻩﺪﺷﻞﻴﻜﺸﺗﺖﻴﻠﭘﺪﻌﺑﻭ ﻪﺘﺨﻳﺭﻥﺁﻞﺧﺍﺩﻪﺑHam’sﻂﻴﺤﻣﺲﭙﺳ .ﺩﻮﺷﻲﻣﺝﺭﺎﺧﻪﻟﻮﻟﻪﺗﺯﺍﻲﻣﺍﺭﺁ

500 ﻥﺁﺯﺍﺲﭘ .ﺪﻧﺎﻤﻧﻰﻗﺎﺑﻪﻟﻮﻟﻪﺗﻰﻟﻮﻠﺳﺎﺗﺩﺩﺮﮔﻲﻣﺝﺭﺎﺧﺍﺩﺪﺠﻣﻭ

ﻮﺷﻭﺖﺴﺷﻥﺁﺎﺑﻪﻟﻮﻟﺭﺍﺪﺟﻭﻪﺘﺨﻳﺭﻪﻟﻮﻟﺭﺩHam’s ﻂﻴﺤﻣﺮﺘﻴﻟﻭﺮﻜﻴﻣ

،ﻂﻴﺤﻣﻦﻳﺍﻥﺩﺮﻛﻪﻓﺎﺿﺍﺎﺑ .ﺩﺩﺮﮔﺭﺎﺑﻥﻭﺪﺑﻪﻟﻮﻟﺭﺍﺪﺟﺎﺗﺩﻮﺷﻰﻣﻩﺩﺍﺩ

.ﺩﺮﻴﮔﻰﻣﺭﺍﺮﻗﻪﻟﻮﻟﻦﻴﻳﺎﭘﺭﺩﻭﻩﺪﺷﺍﺪﺟﻪﻟﻮﻟﺭﺍﺪﺟﻪﺑﻩﺪﻴﺒﺴﭼﻱﺎﻫﻡﺮﭙﺳﺍ

ﻦﻴﻴﻌﺗﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛ،ﻯژﻮﻟﻮﻓﺭﻮﻣﻰﺑﺎﻳﺯﺭﺍﻯﺍﺮﺑﺎﻫﻝﻮﻠﺳﻦﻳﺍﺯﺍﺲﭙﺳ

.(8) ﺪﺷﻩﺩﺎﻔﺘﺳﺍDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻥﺍﺰﻴﻣ

TUNEL assay

ﻱﺎﻫﺮﻴﻤﺳﺍ ﻪﻛﺩﻮﺷﻲﻣﻪﻴﻬﺗﻯﺮﻴﻤﺳﺍ،ﻩﺪﺷﻪﺘﺴﺷﻦﻤﺳﻪﻧﻮﻤﻧﺯﺍﺍﺪﺘﺑﺍﺭﺩ

ﺭﺩﺎﻫﺪﻳﻼﺳﺍﺪﻌﺑﻪﻠﺣﺮﻣﺭﺩﺪﻧﻮﺷﻚﺸﺧﺍﻮﻫﺽﺮﻌﻣﺭﺩﺪﻳﺎﺑﻩﺪﺷﻪﻴﻬﺗ ﻮﺷﻭﺖﺴﺷﺯﺍﺲﭘ .ﺪﻧﻮﺷﻰﻣﺲﻜﻴﻓﻪﻘﻴﻗﺩ 25 ﺕﺪﻣﻯﺍﺮﺑﺪﺻﺭﺩ 4 ﺪﻴﻫﺪﻟﺎﻣﺮﻓ x-100ﻥﻮﺘﻳﺮﺗﺭﺩﺎﻫﺪﻳﻼﺳﺍ Phosphate Buffered Saline ﻭ(PBS) ﺭﺩ

ﻮﺷﻭﺖﺴﺷPBSﺭﺩﺍﺩﺪﺠﻣﻪﺘﻓﺮﮔﺭﺍﺮﻗﻪﻘﻴﻗﺩ 5 ﺕﺪﻣﻯﺍﺮﺑﺪﺻﺭﺩ 0/2

ﻪﻘﻴﻗﺩ 10 ﺎﺗ 5 ﺕﺪﻣﻪﺑ TUNELﺮﻓﺎﺑﺮﺘﻴﻟﻭﺮﻜﻴﻣ 100ﺭﺩﺎﻫﺪﻳﻼﺳﺍ .ﺪﻧﺩﺮﮔﻰﻣ

ﻦﻳﺍﺯﺍﺲﭘ .ﺪﻧﻮﺷﻰﻣﺭﺍﺩﻥﺎﺸﻧ_rTdTﻝﻮﻠﺤﻣﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑﺲﭙﺳﻪﺘﻓﺮﮔﺭﺍﺮﻗ

ﻪﻘﻴﻗﺩ 60 ﺕﺪﻣﻪﺑ،ﻪﺘﻓﺮﮔﺭﺍﺮﻗﺹﻮﺼﺨﻣﻰﻜﻴﺘﺳﻼﭘﺭﻭﺎﻛﺭﺩﺎﻫﺪﻳﻼﺳﺍﻪﻠﺣﺮﻣ

ﻰﻜﻴﺘﺳﻼﭘﺭﻭﺎﻛﺲﭙﺳ .ﺪﻧﻮﺷﻰﻣﻪﺑﻮﻜﻧﺍﻪﺟﺭﺩ 37 ﻯﺎﻣﺩﺭﺩﻭﺭﻮﻧﺯﺍﺭﻭﺩ

ﺲﭘﺪﻧﺮﻴﮔﻰﻣﺭﺍﺮﻗﻪﻘﻴﻗﺩ 15 ﺕﺪﻣﻪﺑ2xsscﺭﺩﺎﻫﺪﻳﻼﺳﺍ،ﻩﺪﺷﻪﺘﺷﺍﺩﺮﺑ

ﻥﺪﺷﻪﺘﺷﺍﺩﺮﺑﺐﺟﻮﻣPBSﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑﺎﻫﺪﻳﻼﺳﺍﻯﻮﺷﻭﺖﺴﺷﻪﻠﺣﺮﻣﺯﺍ

ﺲﭙﺳ .ﺩﺩﺮﮔﻰﻣ ﺎﻫﺪﻳﻼﺳﺍﻯﻭﺭ ﺯﺍﻰﻓﺎﺿﺍ Fluorescein-12-dUTP Propidium Iodideﻭ(PI) ﺖﻨﺳﺭﻮﻠﻓﮓﻧﺭﻂﺳﻮﺗﺎﻫﺪﻳﻼﺳﺍﻯﺰﻴﻣﺁﮓﻧﺭ

ﺮﻳﺯ ﺭﺩ ﺲﭙﺳ ﻭ ﻩﺪﺷ ﺖﻧﻮﻣ PBS ﺎﺑ ﺎﻫﺪﻳﻼﺳﺍ .ﺩﺮﻴﮔﻰﻣ ﻡﺎﺠﻧﺍ

ﻰﻣﺎﻤﺗPIﮓﻧﺭ ﺪﻧﺮﻴﮔﻰﻣﺭﺍﺮﻗﻰﺳﺭﺮﺑﺩﺭﻮﻣﺖﻨﺳﺭﻮﻠﻓپﻮﻜﺳﻭﺮﻜﻴﻣ

ﮓﻧﺭﻭﺩﺭﻭﺁﻰﻣﺭﺩﺰﻣﺮﻗﮓﻧﺭﻪﺑﺍﺭDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺪﻗﺎﻓﻯﺎﻫﻝﻮﻠﺳ

ﻪﻧﻮﻤﻧﺮﻫﺭﺩ .ﺪﻧﻮﺷﻰﻣﻩﺪﻫﺎﺸﻣ،ﺪﻧﺭﺍﺩﻚﻴﺘﭘﻮﭘﺁﻪﺘﺴﻫﻪﻛﻰﻳﺎﻫﻝﻮﻠﺳﺭﺩﺰﺒﺳ

ﺵﺭﺍﺰﮔ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺪﺻﺭﺩﺕﺭﻮﺻﻪﺑﻭﺵﺭﺎﻤﺷﻝﻮﻠﺳ 200

.(14) ﺩﺩﺮﮔﻰﻣ

ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﺶﺠﻨﺳ ﻝﻮﻧﺎﺘﻣ) ﻱﻮﻧﺭﺎﻛﻝﻮﻠﺤﻣﺭﺩﻪﻛﻲﻣﺮﭙﺳﺍﻊﻳﺎﻣﺯﺍﻩﺪﺷﻩﺩﺎﻣﺁﻱﺎﻫﺮﻴﻤﺳﺍ

ﺕﺪﻣﻪﺑ،ﺪﻧﺍﻩﺪﺷﺲﻜﻴﻓﻢﺠﺣﻢﻫﺭﻮﻃﻪﺑ (1 : 3 ﺖﺒﺴﻧﺎﺑﻚﻴﺘﺳﺍﺪﻴﺳﺍﻭ

ﻡﺎﺠﻧﺍ ﺭﻮﻈﻨﻣﻪﺑ .ﺩﺮﻴﮔﻰﻣﺭﺍﺮﻗﺩﺍﺮﮔﻲﺘﻧﺎﺳ ﻪﺟﺭﺩ 4 ﻱﺎﻣﺩﺭﺩ ﻪﻘﻴﻗﺩ 5

ﺯﺍﺮﺘﻴﻟﻭﺮﻜﻴﻣ 100 ﺎﺑﻪﻘﻴﻗﺩ 20 ﺕﺪﻣﻪﺑﺪﻳﻼﺳﺍﺮﻫ_CMA3ﻱﺰﻴﻣﺁﮓﻧﺭ

ﺮﻓﺎﺑﺭﺩﺮﺘﻴﻟﻲﻠﻴﻣﺮﺑﻡﺮﮔﻰﻠﻴﻣ 0/25) ﻩﺪﺷﻱﺰﻴﻣﺁﮓﻧﺭ CMA3ﻝﻮﻠﺤﻣ

ﺯﺍﻲﺳﻲﺳ 32/9 ﻭ 0/1 ﻚﻴﺘﺳﺍﺪﻴﺳﺍﻲﺳﻲﺳ 7 ﻞﻣﺎﺷﻪﻛ :ﻦﻳﻮﻠﻛﻚﻣ

ﻡﺮﮔﻰﻠﻴﻣ 95 ﻭpH=7 ﺭﻻﻮﻣ 0/2 ﺖﻈﻠﻏﺎﺑﻭ 4 Na₂HPOﻭ 7 H₂O

ﻮﺷﻭﺖﺴﺷPBSﺮﻓﺎﺑﺎﺑﺍﺭﺎﻫﺪﻳﻼﺳﺍ .(ﺪﺷﺎﺑﻲﻣﺭﻻﻮﻣﻲﻠﻴﻣ 10 _Mgcl

2

ﺰﻴﻟﺎﻧﺁ .ﺪﻧﻮﺷﻰﻣ ﺖﻧﻮﻣ ﻝﻭﺮﺴﻴﻠﮔ ﻚﻤﻛ ﻪﺑ ﺎﻫﺪﻳﻼﺳﺍ ﺲﭙﺳ ،ﻩﺩﺍﺩ

ﻪﻧﻮﻤﻧﺮﻫﺭﺩﻭﺖﻨﺳﺭﻮﻠﻓپﻮﻜﺳﻭﺮﻜﻴﻣﻂﺳﻮﺗﺎﻫﺪﻳﻼﺳﺍﻲﭘﻮﻜﺳﻭﺮﻜﻴﻣ ﻱﺎﻫﻡﺮﭙﺳﺍ،CMA3ﻱﺰﻴﻣﺁﮓﻧﺭﺯﺍﺲﭘ .ﺪﻳﺩﺮﮔﻰﺑﺎﻳﺯﺭﺍﻝﻮﻠﺳ 200

ﺎﺑ ﻰﻌﻴﺒﻃﻱﺎﻫﻡﺮﭙﺳﺍﻭﻥﺎﺸﺧﺭﺩﺩﺭﺯﮓﻧﺭﺎﺑﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛﻯﺍﺭﺍﺩ

. (19)ﺪﻨﺷﺎﺑﻰﻣﻩﺪﻫﺎﺸﻣﻞﺑﺎﻗﮓﻧﺭﻢﻛﺩﺭﺯﮓﻧﺭ

Sperm Chromatin Dispersion (SCD)

ﺎﺗ 5 ﺖﻈﻠﻏ) ﻩﺪﺷ ﻩﺩﺎﻣﺁ ﻰﻣﺮﭙﺳﺍ ﻪﻧﻮﻤﻧ ﺯﺍ ﺮﺘﻴﻟﻭﺮﻜﻴﻣ 30ﺭﺍﺪﻘﻣ

ﻦﻴﻳﺎﭘﺏﻭﺫﻪﺟﺭﺩﺎﺑﺪﺻﺭﺩ1 ﺯﻭﺭﺎﮔﺁﺯﺍﺮﺘﻴﻟﻭﺮﻜﻴﻣ 70 ﺎﺑ (ﻥﻮﻴﻠﻴﻣ 10

ﻁﻮﻠﺨﻣ ﺩﺍﺮﮔﻰﺘﻧﺎﺳ ﻪﺟﺭﺩ 37 ﻯﺎﻣﺩ ﺭﺩ _{(Low Melting Agaros)}

ﺯﻭﺭﺎﮔﺁﺎﺑﻞﺒﻗﺯﺍﻪﻛﻲﻣﻻﻱﻭﺭﺮﺑﻩﺪﺷﻁﻮﻠﺨﻣﻪﻧﻮﻤﻧﺲﭙﺳ .ﺩﺩﺮﮔﻲﻣ

ﻱﻭﺭﺮﺑﻞﻣﻻﻚﻳﻦﺘﺷﺍﺬﮔﺎﺑﻭﺩﺮﻴﮔﻲﻣﺭﺍﺮﻗ،ﻩﺪﺷﻩﺪﻴﺷﻮﭘﺪﺻﺭﺩ 0/65 .ﺩﻮﺷﻰﻣﻪﺘﺷﺍﺬﮔ،ﺩﺍﺮﮔﻲﺘﻧﺎﺳ ﻪﺟﺭﺩ 4 ﻱﺎﻣﺩﺭﺩﻪﻘﻴﻗﺩ 5 ﺕﺪﻣﻪﺑﻥﺁ

ﺭﺩﻲﻘﻓﺍﺕﺭﻮﺻﻪﺑﻡﻻﺮﻫﻭﻩﺩﺮﻛﺍﺪﺟﻡﻻﺢﻄﺳﺯﺍﺍﺭﻞﻣﻻﺖﻗﺩﺎﺑ

.ﺩﻮﺷﻲﻣﻩﺩﺍﺩﺭﺍﺮﻗﻪﻘﻴﻗﺩ 7 ﺕﺪﻣﻪﺑﻝﺎﻣﺮﻧ 0/08 ﻚﻳﺭﺪﻳﺮﻠﻛﺪﻴﺳﺍﻝﻮﻠﺤﻣ

ﻪﻘﻴﻗﺩ 25 ﺕﺪﻣ ﻪﺑ (pH=7/5) ﻩﺪﻨﻨﻛﺰﻴﻟﻝﻮﻠﺤﻣﺭﺩﻪﻠﺣﺮﻣﻦﻳﺍﺯﺍﺪﻌﺑ

ﺐﻴﺗﺮﺗﻪﺑﺮﻄﻘﻣﺏﺁﺭﺩﻮﺷﻭﺖﺴﺷﺭﺎﺑﻭﺩﺯﺍﺲﭘ .(22) ﺩﻮﺷﻰﻣﻪﺘﺷﺍﺬﮔ

ﻪﻘﻴﻗﺩ 2 ﺕﺪﻣﻪﺑﻡﺍﺪﻛﺮﻫﺪﺻﺭﺩ 100ﻭﺪﺻﺭﺩ 90 ﻭﺪﺻﺭﺩ 70 ﻞﻜﻟﺍﺭﺩ Wrightﮓﻧﺭﻝﻮﻠﺤﻣﺎﺑﺎﻫﻡﻻﻥﺪﺷﻚﺸﺧﺯﺍﺪﻌﺑ،ﺩﺮﻴﮔﻰﻣﻯﺮﻴﮔﺏﺁ

ﻂﺳﻮﺗﺎﻫﻡﻻﺲﭙﺳ .ﺩﺮﻴﮔﻲﻣﺕﺭﻮﺻﻱﺰﻴﻣﺁﮓﻧﺭ 1:1 ﺖﺒﺴﻧﻪﺑ PBSﻭ .ﺩﻮﺷﻲﻣﺵﺭﺎﻤﺷﻡﺮﭙﺳﺍ 200 ﻪﻧﻮﻤﻧﺮﻫﺭﺩﻭﻲﺳﺭﺮﺑﻱﺭﻮﻧپﻮﻜﺳﻭﺮﻜﻴﻣ

ﺕﺭﻮﺻﻪﺑﺍﺭDNAﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓﻥﺍﺰﻴﻣﻥﺍﻮﺗﻲﻣﺵﻭﺭﻦﻳﺍﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑ

ﻪﺑﺖﺒﺴﻧﻥﺁﻩﺯﺍﺪﻧﺍﻭﻪﺘﺴﻫﻑﺍﺮﻃﺍﻪﻟﺎﻫﺩﻮﺟﻭﻪﺑﻪﺟﻮﺗﺎﺑﻝﻮﻠﺳﻉﻮﻧ 5

ﻡﺮﭙﺳﺍﻪﺘﺴﻫ) DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺎﺑﻱﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩ .ﺩﻮﻤﻧﻲﺳﺭﺮﺑﻪﺘﺴﻫ

ﻥﻭﺪﺑﻭ (ﻩﺪﺷDegradeﻡﺮﭙﺳﺍﻝﻮﻠﺳﻭ ﻪﻟﺎﻫﻥﻭﺪﺑ،ﻚﭼﻮﻛﻪﻟﺎﻫﺎﺑ

ﺮﻫﺭﺩ (ﻂﺳﻮﺘﻣﻪﻟﺎﻫﻭگﺭﺰﺑﻪﻟﺎﻫﺎﺑﻡﺮﭙﺳﺍﻪﺘﺴﻫ) DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

ﻪﻧﻮﻤﻧﺮﻫﺭﺩDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺪﺻﺭﺩﺕﺭﻮﺻﻪﺑﻭﻩﺪﺷﻲﺑﺎﻳﺯﺭﺍﻪﻧﻮﻤﻧ .(1ﻞﻜﺷ) ﺩﻮﺷﻰﻣﺵﺭﺍﺰﮔ

Acridin orange staining

ﺭﺩ،ﺍﻮﻫﺽﺮﻌﻣﺭﺩﻥﺪﺷﻚﺸﺧﺯﺍﺲﭘﺎﻫﺮﻴﻤﺳﺍﺵﻭﺭﻦﻳﺍﺭﺩ

ﺯﺍﺭﻭﺩ (1 : 3 ﺖﺒﺴﻧﺎﺑﻚﻴﺘﺳﺍﺪﻴﺳﺍﻭﻝﻮﻧﺎﺘﻣ) ﻯﻮﻧﺭﺎﻛﻮﻴﺗﺎﺴﻜﻴﻓﻝﻮﻠﺤﻣ

ﻝﻮﻠﺤﻣﺎﺑﺲﭙﺳ،ﻩﺪﺷﺲﻜﻴﻓﺖﻋﺎﺳ 2 ﺕﺪﻣﻪﺑﻪﺟﺭﺩ 4 ﻯﺎﻣﺩﺭﺩﻭﺭﻮﻧ

ﺭﺩﻩﺪﺷﻖﻴﻗﺭﺪﺻﺭﺩ0/1 ﺞﻧﺭﻭﺍﻦﻳﺪﻳﺮﻛﺍﻲﺳﻲﺳ 10) ﺞﻧﺭﻭﺍﻦﻳﺪﻳﺮﻛﺁ

ﻝﻮﻠﺤﻣﺯﺍﻲﺳﻲﺳ 2/5 ﻭﺭﻻﻮﻣ 0/1 ﻚﻴﺘﺳﺍﺪﻴﺳﺍﻲﺳﻲﺳ 40 ،ﺮﻄﻘﻣﺏﺁ .ﺪﻧﻮﺷﻰﻣﻯﺰﻴﻣﺁﮓﻧﺭﻪﻘﻴﻗﺩ 10 ﺎﺗ 7 ﺕﺪﻣﻪﺑ (ﺭﻻﻮﻣ 0/3 Na2HPO4

.ﺪﻳﺩﺮﮔﻲﻣﺖﻧﻮﻣPBSﻪﻠﻴﺳﻭﻪﺑﻭﻩﺪﺷﻩﺩﺍﺩﻮﺷﻭﺖﺴﺷﺎﻫﺪﻳﻼﺳﺍﺲﭙﺳ

200 ﻪﻧﻮﻤﻧﺮﻫﺭﺩﻭﻰﺳﺭﺮﺑﺖﻨﺳﺭﻮﻠﻓپﻮﻜﺳﻭﺮﻜﻴﻣﺮﻳﺯﺭﺩﺎﻫﺪﻳﻼﺳﺍ

ﺰﻣﺮﻗﮓﻧﺭﻪﺑﻯﺍﻪﺘﺷﺭﻚﺗ DNAﻯﺰﻴﻣﺁﮓﻧﺭﻦﻳﺍﺭﺩ .ﺪﺷﺵﺭﺎﻤﺷﻡﺮﭙﺳﺍ

.(23) ﺩﺩﺮﮔﻰﻣﻩﺪﻫﺎﺸﻣﺰﺒﺳﮓﻧﺭﻪﺑﻯﺍﻪﺘﺷﺭﻭﺩﻰﻌﻴﺒﻃ DNAﻭ

ﻱﺭﺎﻣﺁﺰﻴﻟﺎﻧﺁ

11/5 ﺭﺍﺰﻓﺍﻡﺮﻧﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑﺎﻫﻩﺩﺍﺩﺰﻴﻟﺎﻧﺁ،ﺞﻳﺎﺘﻧﻱﺭﻭﺁﻊﻤﺟﺯﺍﺲﭘ

ﻲﮕﺘﺴﺒﻤﻫﺐﻳﺮﺿﻲﺳﺭﺮﺑﺖﻬﺟﻭﺖﻓﺮﮔﻡﺎﺠﻧﺍ t testﻥﻮﻣﺯﺁﻭSPSS

ﻯﺩﻮﺒﻬﺑ ﺪﺻﺭﺩ ﻦﻴﻨﭼﻢﻫ .ﺪﺷﻩﺩﺎﻔﺘﺳﺍ Correlation Coefficient ﺯﺍ

ﻭZetaﺵﻭﺭﻭﺩﺎﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ :ﺪﻳﺩﺮﮔﻪﺒﺳﺎﺤﻣﺮﻳﺯﺕﺭﻮﺻﻪﺑ DGC Control-Zeta/Zeta×100 Control-DGC/DGC×100

ﺎﻫ

ﻪﺘﻓﺎﻳ

ﻯﺭﻭﺭﺎﺑﺰﻛﺮﻣ ﻪﺑ ﻩﺪﻨﻨﻛﻪﻌﺟﺍﺮﻣﺭﻭﺭﺎﺑﺎﻧ ﺩﺮﻓ 63 ﺯﺍﻪﻌﻟﺎﻄﻣﻦﻳﺍ ﺭﺩ

ﻞﻣﺎﺷ ﻰﻣﺮﭙﺳﺍ ﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﻦﻴﮕﻧﺎﻴﻣ .ﺪﺷ ﻩﺩﺎﻔﺘﺳﺍ ﻥﺎﻬﻔﺻﺍ ﻯﺭﻭﺭﺎﺑﺎﻧ ﻭ

ﺪﺻﺭﺩﻭ 49/61 _± 13/18 ﻡﺮﭙﺳﺍﻙﺮﺤﺗ،55/7 _± 20/37 ﻡﺮﭙﺳﺍﺖﻈﻠﻏ

.ﺖﺳﺍ 69/83_±11/24 ﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍ

ﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻦﻴﮕﻧﺎﻴﻣ،1 ﻩﺭﺎﻤﺷﻝﻭﺪﺟﻪﺑﻪﺟﻮﺗﺎﺑ

ﻭ 80/92 _± 6/01 Zeta ﻩﻭﺮﮔ ،86/06 _± 5/33ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﺭﺩ

ﻯﺎﻫﻡﺮﭙﺳﺍ ﻦﻴﮕﻧﺎﻴﻣ ﻩﻭﻼﻋ ﻪﺑ .ﺖﺳﺍ 76/82 _± 12/14 DGC ﻩﻭﺮﮔ

9/29 Zetaﻩﻭﺮﮔ،41/37 _± 11/03 ﻝﺮﺘﻨﻛﻩﻭﺮﮔﺭﺩﺖﺒﺜﻣCMA3

ﺪﺻﺭﺩﻦﻴﮕﻧﺎﻴﻣ .ﺪﺷﺎﺑﻰﻣ 32/49 _± 10/34 DGCﻩﻭﺮﮔﻭ 32/07 _±

ﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩﺖﺒﺜﻣCMA3 ﻭﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍ

ﻪﻛﻪﺘﻓﺎﻳﺶﻫﺎﻛ ﻝﺮﺘﻨﻛﻩﻭﺮﮔ ﻪﺑﺖﺒﺴﻧDGCﻭZeta ﺵﻭﺭﻭﺩﻪﺑ

ﺭﺩﻦﻴﻨﭽﻤﻫ .(p<0/001)ﺖﺳﺍﺭﺍﺩﻰﻨﻌﻣﻯﺭﺎﻣﺁﺮﻈﻧﺯﺍ ﻑﻼﺘﺧﺍﻦﻳﺍ

ﻯﺎﻫﻡﺮﭙﺳﺍ ﺭﺩ،ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩ ﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣﺕﺭﻮﺻ

ﺶﻫﺎﻛZetaﺵﻭﺭﻪﺑﺖﺒﺴﻧ DGCﺵﻭﺭﺭﺩﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍ

.(p<0/005) ﺖﺳﺍﻩﺩﺍﺩﻥﺎﺸﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ ﻯﺎﻫﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ ﻦﻴﮕﻧﺎﻴﻣ 2ﻩﺭﺎﻤﺷ ﻝﻭﺪﺟ

ﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ AO ﻭTUNEL ،SCD ﻚﻴﻨﻜﺗ ﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑ DNA

ﺍﺭﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑﺖﺒﺴﻧZetaﻭ DGCﺵﻭﺭﻭﺩﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩ ﻯﺎﻫﻡﺮﭙﺳﺍ ﺪﺻﺭﺩﻦﻴﮕﻧﺎﻴﻣ .ﺪﻫﺩﻰﻣﻥﺎﺸﻧ

Zeta ﻩﻭﺮﮔ،15/83 _± 8/14 ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﺭﺩ TUNEL ﺵﻭﺭ ﻪﺑ

ﻪﻛﻰﺗﺭﻮﺻﺭﺩ .ﺖﺳﺍ 8/17 _± 4/23 DGCﻩﻭﺮﮔﻭ 5/89 _± 3/92 DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻦﻴﮕﻧﺎﻴﻣ،SCDﺵﻭﺭﺭﺩ

29/21 _± 14/95 Zetaﻩﻭﺮﮔﺭﺩ،50/43 _± 15/58 ﻝﺮﺘﻨﻛﻩﻭﺮﮔﺭﺩ

ﺪﺻﺭﺩ ﻦﻴﮕﻧﺎﻴﻣﻩﻭﻼﻋﻪﺑ .ﺖﺳﺍ 33/58 _± 16/24 DGCﻩﻭﺮﮔﺭﺩ ﻭ

ﻝﺮﺘﻨﻛﻩﻭﺮﮔﺭﺩAOﺵﻭﺭﻪﺑDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍ

DGCﻩﻭﺮﮔﺭﺩ ﻭ 28/85 _± 15/83 Zetaﻩﻭﺮﮔ،49/65 _± 15/1

ﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻦﻴﮕﻧﺎﻴﻣﺞﻳﺎﺘﻧﻦﻳﺍﻪﺑﻪﺟﻮﺗﺎﺑ .ﺖﺳﺍ 32/56 _± 14/38 TUNEL ﻚﻴﻨﻜﺗ ﻪﺳ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ

ﻪﺑﺖﺒﺴﻧZetaﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ AOﻭ،SCD

ﻥﺎﺸﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﻑﻼﺘﺧﺍDGCﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍ

.(p<0/005) ﺪﻫﺩﻰﻣ

ﻯﺭﺍﺩﻰﻨﻌﻣﻁﺎﺒﺗﺭﺍ،ﻰﻣﺮﭙﺳﺍﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘﻥﺎﻴﻣﺯﺍ 3 ﻝﻭﺪﺟﺱﺎﺳﺍﺮﺑ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﺎﺑ (ﻙﺮﺤﺗ ﻭ ﺖﻈﻠﻏ) ﻰﻣﺮﭙﺳﺍ ﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﻦﻴﺑ

ﺰﻴﻧﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﺎﺑ ﻡﺮﭙﺳﺍﺖﻈﻠﻏﻦﻴﺑ ﻪﻄﺑﺍﺭﻦﻴﻨﭼﻢﻫ .ﺩﺭﺍﺪﻧﺩﻮﺟﻭ

ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻥﺍﺰﻴﻣﺎﺑﻡﺮﭙﺳﺍﻙﺮﺤﺗﻪﻛﻰﻟﺎﺣﺭﺩ .ﺖﺴﻴﻧﺭﺍﺩﻰﻨﻌﻣ

.(p=0/001) ﺩﺭﺍﺩﻯﺭﺍﺩﻰﻨﻌﻣﻁﺎﺒﺗﺭﺍ

ﺭﻮﻴﭘ ﻭ ﺎﺗﺯ ﺵﻭﺭ ﻭﺩ ﻪﺑ ﻩﺪﺷ ﻯﺯﺎﺳﺍﺪﺟ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ،ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﻪﺳ ﺭﺩ ﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ ﻭ ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯژﻮﻟﻮﻓﺭﻮﻣ ﺎﺑ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣ :1ﻝﻭﺪﺟ

P-value

(P) ﺭﻮﻴﭘ

(Z) ﺎﺗﺯ

(C) ﻝﺮﺘﻨﻛ

ﺎﻫﺮﺘﻣﺍﺭﺎﭘ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

C-Z: 0/001

C-P: _0/001

Z-P: 0/005

76/82 ± 12/14

80/92 ± 6/01

86/06 ± 5/33

ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

(Diff Quick)

C-Z: 0/001

C-P: 0/001

Z-P: 0/917

32/49 ± 10/34

32/07 ± 9/29

41/37 ± 11/03

CMA3+ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﺭﻮﻴﭘ ﻭ ﺎﺗﺯ ﺵﻭﺭ ﻭﺩ ﻪﺑ ﻩﺪﺷ ﻯﺯﺎﺳﺍﺪﺟ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ،ﻝﺮﺘﻨﻛ ﻩﻭﺮﮔ ﻪﺳ ﺭﺩ DNA ﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣ :2ﻝﻭﺪﺟ

P-value

(P) ﺭﻮﻴﭘ

(Z) ﺎﺗﺯ

(C) ﻝﺮﺘﻨﻛ

ﺎﻫﺮﺘﻣﺍﺭﺎﭘ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

ﺭﺎﻴﻌﻣ ﻑﺍﺮﺤﻧﺍ ± ﻦﻴﮕﻧﺎﻴﻣ

C-Z: 0/001

C-P: _0/001

Z-P: 0/001

8/17 ± 4/23

5/89± 3/92

15/83 ± 8/14

ﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (TUNEL)

C-Z: 0/001

C-P: _0/001

Z-P: 0/001

32/56 ± 14/38

28/85± 15/83

49/65 ± 15/1

ﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (AO)

C-Z: 0/002

C-P: _0/001

Z-P: 0/001

33/58 ±16/24

29/21 ±14/95

50/43 ± 15/58

ﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓ ﻯﺍﺭﺍﺩ ﻯﺎﻬﻣﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (SCD)

DNA ﻥﻮﻴﺳﺎﺘﻧﺎﻤﮔﺍﺮﻓ ﻭ ﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ ﺎﺑ ﻰﻣﺮﭙﺳﺍ ﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ :3ﻝﻭﺪﺟ

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (SCD)

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (AO)

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (TUNEL)

ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

CMA3+

ﻰﻣﺮﭙﺳﺍ ﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

0/053 0/281 0/314 0/135 0/359 0/130 0/404 -0/122

106_/mlﻡﺮﭙﺳﺍﺖﻈﻠﻏ

0/583 0/081 0/466 -0/098 0/305 0/145 0/001** -0/445 ﻡﺮﭙﺳﺍ ﻙﺮﺤﺗ ﺪﺻﺭﺩ

0/001** 0/777 0/001** 0/566 0/001** 0/541 0/001** 0/782

ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

WHO ﻰﻌﻴﺒﻃﺮﻴﻏ

0/561 -0/084 0/410 0/109 0/298 -0/146 0/590 0/077

ﻯﺎﻫﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

Diff Quickﻰﻌﻴﺒﻃﺮﻴﻏ

.ﺖﺳﺍ p<0/01 ﺎﺑ ﺮﺘﻣﺍﺭﺎﭘ ﻭﺩ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ ﻥﺩﻮﺑ ﺭﺍﺩ ﻰﻨﻌﻣ ﺮﮕﻧﺎﻴﺑ ** .ﺖﺳﺍ p<0/05 ﺎﺑ ﺮﺘﻣﺍﺭﺎﭘ ﻭﺩ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ ﻥﺩﻮﺑ ﺭﺍﺩ ﻰﻨﻌﻣ ﺮﮕﻧﺎﻴﺑ *

ﻁﺎﺒﺗﺭﺍ WHO ﻯﺎﻫﺭﺎﻴﻌﻣ ﺱﺎﺳﺍ ﺮﺑ ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯﺎﻫﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﺖﺳﺍﻩﺩﺍﺩﻥﺎﺸﻧDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﺎﺑﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣ

ﭻﻴﻫDiff Quickﺵﻭﺭﻪﺑﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﺎﻣﺍ،(p=0/001) DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻭ ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻥﺍﺰﻴﻣﺎﺑﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﻁﺎﺒﺗﺭﺍ

ﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻥﺍﺰﻴﻣﻦﻴﺑﻁﺎﺒﺗﺭﺍﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩﻩﻭﻼﻋﻪﺑ .ﺪﻫﺩﻰﻤﻧﻥﺎﺸﻧ

ﺭﺍﺩﻰﻨﻌﻣSCDﻭ TUNEL ،AOﻚﻴﻨﻜﺗﻪﺳﺮﻫﺭﺩDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

.(p<0/001 ) ﺖﺳﺍ

DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻦﻴﺑﻁﺎﺒﺗﺭﺍ 4 ﻩﺭﺎﻤﺷﻝﻭﺪﺟﺱﺎﺳﺍﺮﺑﻦﻴﻨﭼﻢﻫ

ﺰﻴﻧ ﺮﮕﻳﺪﻜﻳﻪﺑ ﺖﺒﺴﻧ SCD ﻭ TUNEL ،AO ﻚﻴﻨﻜﺗ ﻪﺳﺮﻫ ﺭﺩ

.(p=0/001) ﺖﺳﺍﺭﺍﺩﻰﻨﻌﻣ

ﻪﻛﺩﺍﺩﻥﺎﺸﻧ 5 ﻩﺭﺎﻤﺷ ﻝﻭﺪﺟﻖﺑﺎﻄﻣﻪﻌﻟﺎﻄﻣﻦﻳﺍﺯﺍﻞﺻﺎﺣﺞﻳﺎﺘﻧ

ﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩDNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺩﻮﺒﻬﺑﺪﺻﺭﺩ

ﺖﺒﺴﻧﺍﺭﻱﺍﻪﻈﺣﻼﻣﻞﺑﺎﻗﺶﻫﺎﻛﻝﺮﺘﻨﻛﻩﻭﺮﮔﺎﺑﻪﺴﻳﺎﻘﻣﺭﺩZetaﺵﻭﺭ .ﺪﻫﺩﻲﻣﻥﺎﺸﻧ،DGCﺵﻭﺭﻪﺑﻩﺪﺷﻱﺯﺎﺳﺍﺪﺟﻱﺎﻫﻡﺮﭙﺳﺍﻪﺑ

ﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺩﻮﺒﻬﺑﺪﺻﺭﺩ :5ﻝﻭﺪﺟ

ﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑﺖﺒﺴﻧﺭﻮﻴﭘﻭﺎﺗﺯﺵﻭﺭﻭﺩﻪﺑﻩﺪﺷ

ﺭﻮﻴﭘ ﺎﺗﺯ

ﺮﺘﻣﺍﺭﺎﭘ

46%

62%

TUNEL

34%

42%

SCD

34%

41%

AOT

ﺚﺤﺑ

ﻩﺩﺮﺑﺭﺎﻛﻪﺑDGCﺵﻭﺭﺭﺩﻪﻛﻰﻧﻮﮔﺎﻧﻮﮔﻯﺭﺎﺠﺗﺩﺍﻮﻣﻥﺎﻴﻣﺯﺍ

ﻚﻤﻛ ﻯﺎﻫﻚﻴﻨﻜﺗ ﺭﺩ ﻝﻮﻤﻌﻣ ﺭﻮﻃ ﻪﺑ Pure Sperm ،ﺩﻮﺷﻰﻣ

.(4) ﺩﺮﻴﮔﻰﻣﺭﺍﺮﻗﻩﺩﺎﻔﺘﺳﺍﺩﺭﻮﻣﻯژﻮﻟﻭﺭﺪﻧﺁﻯﺎﻫﻩﺎﮕﺸﻳﺎﻣﺯﺁﻭﻯﺭﻭﺭﺎﺑ

ﺯﺍﻩﺩﺎﻔﺘﺳﺍﻪﻛﺩﺍﺩﻥﺎﺸﻧ 2000 ﻝﺎﺳﺭﺩﺶﻧﺍﺭﺎﻜﻤﻫﻭﺱﺎﻛﺎﺳﺕﺎﻘﻴﻘﺤﺗ

ﺪﺻﺭﺩﺭﺩﺍﺭﻯﺍﻪﻈﺣﻼﻣﻞﺑﺎﻗﺶﻫﺎﻛ،ﻡﺮﭙﺳﺍﻯﺯﺎﺳﻩﺩﺎﻣﺁﺭﺩPure Sperm

(5) ﺪﻳﺎﻤﻧﻰﻣﺩﺎﺠﻳﺍ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻯﺍﺭﺍﺩﻭﺖﺒﺜﻣCMA3ﻯﺎﻫﻡﺮﭙﺳﺍ

ﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺭﺩ،ﻰﻌﻴﺒﻃﻯژﻮﻟﻮﻓﺭﻮﻣﺎﺑﺎﻫﻡﺮﭙﺳﺍﺖﺒﺴﻧﻦﻴﻨﭼﻢﻫﻭ ﻪﻠﻤﺟﺯﺍﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﺵﻭﺭﺮﻳﺎﺳﺎﺑﻪﺴﻳﺎﻘﻣﺭﺩDGCﺵﻭﺭﻪﺑ

.(5) ﺪﺑﺎﻳﻰﻣﺶﻳﺍﺰﻓﺍﻯﺭﺍﺩﻰﻨﻌﻣﺭﻮﻃﻪﺑSwim-up

2006 ﻝﺎﺳ ﺭﺩ ﺶﻧﺍﺭﺎﻜﻤﻫ ﻭ ﻥﺎﭼ ﻂﺳﻮﺗ ﻪﻛ Zeta ﺵﻭﺭ ﺭﺩ

ﻱﻭﺭ ﻲﻜﻳﺮﺘﻜﻟﺍ ﻞﻴﺴﻧﺎﺘﭘﺱﺎﺳﺍ ﺮﺑﻎﻟﺎﺑﻡﺮﭙﺳﺍ ﺏﺎﺨﺘﻧﺍ ،ﺪﻳﺩﺮﮔ ﻰﻓﺮﻌﻣ ﻥﺎﺸﻧﺶﻧﺍﺭﺎﻜﻤﻫﻭﻥﺎﭼﺕﺎﻌﻟﺎﻄﻣ .(8) ﺩﺮﻴﮔﻰﻣﺕﺭﻮﺻﻡﺮﭙﺳﺍﻱﺎﺸﻏ

ﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﺏﺎﺨﺘﻧﺍﻥﺎﻜﻣﺍ،Zetaﺵﻭﺭﻪﺑﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﻪﻛﺩﺍﺩ

ﻪﻛﻰﻳﺎﺠﻧﺁﺯﺍ .(8) ﺪﻳﺎﻤﻧﻰﻣﻢﻫﺍﺮﻓﺍﺭﺮﺘﺸﻴﺑﻙﺮﺤﺗﻭﺮﺘﻬﺑﻯژﻮﻟﻮﻓﺭﻮﻣ

ﻭ DGCﺵﻭﺭﻭﺩﻰﻳﺍﺭﺎﻛﻖﻴﻗﺩﻪﺴﻳﺎﻘﻣﻭﻰﺳﺭﺮﺑﻪﺑﻯﺍﻪﻌﻟﺎﻄﻣﻥﻮﻨﻛﺎﺗ

ﻪﺘﻓﺮﮕﻧﺕﺭﻮﺻ،ﺩﺯﺍﺩﺮﭙﺑﻰﻌﻴﺒﻃ ﻭﻎﻟﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺭﺩZeta

ﻭﺩﻦﻳﺍﺯﺍﻩﺪﻣﺁﺖﺳﺩﻪﺑﻯﺎﻫﻡﺮﭙﺳﺍﺪﺷﻪﺘﻓﺮﮔﻢﻴﻤﺼﺗﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩ،ﺩﻮﺑ ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛ،ﻯژﻮﻟﻮﻓﺭﻮﻣﻅﺎﺤﻟﺯﺍﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭ ﻥﺍﺰﻴﻣﻝﺮﺘﻨﻛﻩﻭﺮﮔﺎﺑﻪﺴﻳﺎﻘﻣﺯﺍﺲﭘﻭﺩﺮﻴﮔﺭﺍﺮﻗﻰﺑﺎﻳﺯﺭﺍﺩﺭﻮﻣDNA

ﺮﻛﺫﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘﺯﺍﻚﻳﺮﻫﺩﻮﺒﻬﺑﺭﺩﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭﻭﺩﻦﻳﺍﻰﻳﺍﺭﺎﻛ

.ﺩﺩﺮﮔﻦﻴﻴﻌﺗﻩﺪﺷ

ﺪﺻﺭﺩ ﻪﻛ ﺪﻫﺩﻰﻣ ﻥﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ ﺯﺍ ﻩﺪﻣﺁ ﺖﺳﺩ ﻪﺑ ﺞﻳﺎﺘﻧ ﺖﺒﺴﻧ ،ﺪﻧﺍﻩﺪﻳﺩﺮﮔ ﻯﺯﺎﺳﺍﺪﺟ DGC ﺵﻭﺭ ﻪﺑ ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯﺎﻫﻡﺮﭙﺳﺍ

ﻭ (p=0/001) ﺪﺑﺎﻳﻰﻣﺶﻫﺎﻛﻯﺭﺍﺩﻰﻨﻌﻣﺕﺭﻮﺻ ﻪﺑﻝﺮﺘﻨﻛ ﻩﻭﺮﮔﻪﺑ

ﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ (CMA3+)ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻥﺍﺰﻴﻣﻦﻴﻨﭽﻤﻫ

ﻥﺎﺸﻧ ﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛ ﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑ ﺖﺒﺴﻧﺵﻭﺭﻦﻳﺍﻪﺑﻩﺪﺷ ﺶﻧﺍﺭﺎﻜﻤﻫ ﻭ ﺱﺎﻛﺎﺳ ﺕﺎﻌﻟﺎﻄﻣ ﺎﺑ ﺎﻫﻪﺘﻓﺎﻳ ﻦﻳﺍ .(p=0/001) ﺪﻫﺩﻰﻣ

SCD, TUNEL, AO ﻚﻴﻨﻜﺗ ﻪﺳ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺎﺑ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻥﺍﺰﻴﻣ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ :4ﻝﻭﺪﺟ

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (TUNEL)

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (SCD)

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

DNA (AO)

ﻰﻣﺮﭙﺳﺍ ﻯﺎﻫﺮﺘﻣﺍﺭﺎﭘ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

P-value

ﺐﻳﺮﺿ

(r) ﻲﮕﺘﺴﺒﻤﻫ

0/001** 0/630

0/001** 0/725

. 1

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (TUNEL) ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

0/001** 0/889

. 1

0/001** 0/725

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (SCD) ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

. 1

0/001** 0/889

0/001** 0/630

ﻯﺍﺭﺍﺩ ﻯﺎﻫ ﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ

DNA (AO) ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

.ﺖﺳﺍ p<0/01 ﺎﺑ ﺮﺘﻣﺍﺭﺎﭘ ﻭﺩ ﻦﻴﺑ ﻁﺎﺒﺗﺭﺍ ﻥﺩﻮﺑ ﺭﺍﺩ ﻰﻨﻌﻣ ﺮﮕﻧﺎﻴﺑ **

:E ،D ،C ،(DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻥﻭﺪﺑ) ﻂﺳﻮﺘﻣ ﻭ گﺭﺰﺑ ﻪﻟﺎﻫ ﺎﺑ ﻡﺮﭙﺳﺍ :B ،A :Sperm Chromatin Dispersion ﺖﺴﺗ :1ﻞﻜﺷ

ﻩﺪﺷﺍﺪﺟ ﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩﻦﻴﻨﭼﻢﻫ .(5) ﺩﺭﺍﺩﺖﻘﺑﺎﻄﻣ 2000 ﻝﺎﺳﺭﺩ

ﻚﻴﻨﻜﺗﻪﺳﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻥﺍﺰﻴﻣDGCﺵﻭﺭﻪﺑ

ﺶﻫﺎﻛﻚﻴﻨﻜﺗﻪﺳﺮﻫﻭﺪﻳﺩﺮﮔﻰﺑﺎﻳﺯﺭﺍAOﻭSCDﻭ TUNEL

ﻝﺮﺘﻨﻛﻩﻭﺮﮔ ﻪﺑ ﺖﺒﺴﻧDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻥﺍﺰﻴﻣﺭﺩ ﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣ

.(p=0/001) ﺩﺍﺩﻥﺎﺸﻧ

ﻦﻤﺳﻪﻧﻮﻤﻧﻪﻛﺩﻮﻤﻧﻩﺭﺎﺷﺍﻪﺘﻜﻧﻦﻳﺍﻪﺑﻥﺍﻮﺗﻰﻣﺞﻳﺎﺘﻧﻦﻳﺍﻪﻴﺟﻮﺗﺭﺩ ﺮﻳﺯﻯﻮﺘﺤﻣ،ﺖﻴﺳﻮﻜﻟﻯﺩﺍﺪﻌﺗﺮﺑﻩﻭﻼﻋﻭﺪﺷﺎﺑﻰﻣﺍﺭﺍﺩﻰﻳﻻﺎﺑﻰﺘﻴﻧژﻭﺮﺘﻫ ﻦﻤﺳﻯﺯﺎﺳﻩﺩﺎﻣﺁ .ﺖﺳﺍﻰﻌﻴﺒﻃﺮﻴﻏﻭﻰﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍﺯﺍﻯﺍﻪﻋﻮﻤﺠﻣ

.(3) ﺪﻳﺎﻤﻧﻰﻣﻢﻫﺍﺮﻓﺍﺭﻰﺘﻴﻧژﻭﺮﺘﻫﻦﻳﺍﺶﻫﺎﻛﻥﺎﻜﻣﺍDGCﺵﻭﺭﻪﺑ

ﻰﻌﻴﺒﻃﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩﻪﻛﺪﻧﺍﻩﺩﺍﺩﻥﺎﺸﻧﻦﻳﺍﺯﺍﺶﻴﭘﻰﻧﻮﮔﺎﻧﻮﮔﺕﺎﻌﻟﺎﻄﻣ ﻯﺎﻫﻪﻳﻻﺭﺩژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺯﺍﺲﭘﺍﺬﻟ .ﺪﺷﺎﺑﻰﻣﺮﺘﺸﻴﺑﻢﺠﺣﻪﺑﻥﺯﻭﺖﺒﺴﻧ

ﻪﻛﻰﻌﻴﺒﻃ ﻦﻴﺗﺎﻣﻭﺮﻛﺭﺎﺘﺧﺎﺳ ﻯﺍﺭﺍﺩ ﻯﺎﻫﻡﺮﭙﺳﺍ ،ﻡﺮﭙﺳﺍ ﺭﻮﻴﭘﻥﺎﻳﺩﺍﺮﮔ ﻪﻛﺪﻧﻮﺷﻰﻣﻦﻴﺸﻧﻪﺗﺮﺗﻊﻳﺮﺳ،ﺪﻧﺭﺍﺩﻯﺮﺘﺸﻴﺑﻰﻟﺎﮕﭼﻭﻩﺩﻮﺑﺮﺗﻢﻛﺍﺮﺘﻣ

.(3-5) ﺩﺩﺮﮔﻰﻣﻰﻣﺮﭙﺳﺍﻪﻧﻮﻤﻧﻥﺪﺷﻦﮕﻤﻫﻪﺑﺮﺠﻨﻣﺩﻮﺧﺮﻣﺍﻦﻳﺍ

ﻯﺩﻮﺒﻬﺑﻥﺍﺰﻴﻣﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧ 5 ﻝﻭﺪﺟﺯﺍﻩﺪﻣﺁﺖﺳﺩﻪﺑﺞﻳﺎﺘﻧ

ﺭﺩSCDﻭ TUNEL ،AOﺵﻭﺭﻪﺳﻪﺑﻩﺪﺷﻯﺮﻴﮔﻩﺯﺍﺪﻧﺍﻡﺮﭙﺳﺍDNA

ﻭﺩﺮﻫﻪﭼﺮﮔﺍ .ﺖﺳﺍﻩﺩﺮﻛﻞﻤﻋDGCﺯﺍﺮﺘﻬﺑZetaﻯﺯﺎﺳﺍﺪﺟﺵﻭﺭ

.(26) ﺪﻨﺘﺴﻫﻢﻟﺎﺳDNAﺎﺑﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﻪﺑﺭﺩﺎﻗﺵﻭﺭ

ﺪﺻﺭﺩﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧﻪﻌﻟﺎﻄﻣﻦﻳﺍﺯﺍﻩﺪﻣﺁﺖﺳﺩﻪﺑﺞﻳﺎﺘﻧﻦﻴﻨﭼﻢﻫ ﺖﺒﺴﻧﺪﻧﺍﻩﺪﻳﺩﺮﮔﻯﺯﺎﺳﺍﺪﺟZetaﺵﻭﺭﻪﺑﻪﻛﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍ

ﻦﻳﺍ .(p=0/001) ﺪﺑﺎﻳﻰﻣﺶﻫﺎﻛﻯﺭﺍﺩﻰﻨﻌﻣﺕﺭﻮﺻﻪﺑﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑ

.(8) ﺩﺭﺍﺩﺖﻘﺑﺎﻄﻣ 2006 ﻝﺎﺳﺭﺩﺶﻧﺍﺭﺎﻜﻤﻫﻭﻥﺎﭼﺕﺎﻌﻟﺎﻄﻣﺎﺑﺎﻫﻪﺘﻓﺎﻳ

ﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩﺰﻴﻧ(CMA3+)ﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻥﺍﺰﻴﻣ

ﺪﻫﺩﻰﻣﻥﺎﺸﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛﻝﺮﺘﻨﻛﻩﻭﺮﮔﺎﺑﻪﺴﻳﺎﻘﻣﺭﺩﺵﻭﺭﻦﻳﺍ ﻥﺍﺰﻴﻣ Zetaﺵﻭﺭﻪﺑ ﻩﺪﺷﺍﺪﺟ ﻯﺎﻫﻡﺮﭙﺳﺍ ﺭﺩﻦﻴﻨﭼﻢﻫ .(p=0/001)

ﻭSCD ﻭ TUNELﻚﻴﻨﻜﺗ ﻪﺳﺯﺍﻩﺩﺎﻔﺘﺳﺍﺎﺑ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ

ﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛﻚﻴﻨﻜﺗﻪﺳﺮﻫﻭﺪﻳﺩﺮﮔﻰﺑﺎﻳﺯﺭﺍAcridin orange

ﺪﻧﺩﺍﺩﻥﺎﺸﻧ ﻝﺮﺘﻨﻛﻩﻭﺮﮔﻪﺑ ﺖﺒﺴﻧDNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻥﺍﺰﻴﻣ ﺭﺩﺍﺭ

.(p<0/001)

ﻦﻳﺍﻪﺑﻥﺍﻮﺗﻰﻣﻪﻌﻟﺎﻄﻣﻦﻳﺍﺭﺩﻩﺪﻣﺁﺖﺳﺩﻪﺑﻯﺎﻫﻪﺘﻓﺎﻳﻪﻴﺟﻮﺗﺭﺩ ﻥﺎﺸﻧ ﻦﻳﺍﺯﺍ ﺶﻴﭘ ﻥﻮﮔﺎﻧﻮﮔﺕﺎﻘﻴﻘﺤﺗ ﻪﭽﻧﺎﻨﭼﻪﻛ ﺩﻮﻤﻧﻩﺭﺎﺷﺍﻪﺘﻜﻧ ﻯﺎﺸﻏﻰﺟﺭﺎﺧ ﺢﻄﺳ ﺮﺑﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﻮﻟﺎﻴﺳﻥﺪﺷﻪﻓﺎﺿﺍ ،ﺪﻧﺍﻩﺩﺍﺩ ﻭﺎﺸﻏﻦﻴﺑ ﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﻑﻼﺘﺧﺍﻩﺪﻧﺭﻭﺁ ﺩﻮﺟﻭﻪﺑ ﻞﻣﺎﻋﻪﻛﻡﺮﭙﺳﺍ

ﺮﻫﺍﺬﻟ .(9) ﺪﻫﺩﻰﻣﺥﺭﺰﻧژﻮﻴﻣﺮﭙﺳﺍﻪﻠﺣﺮﻣﺭﺩ،ﺪﺷﺎﺑﻰﻣﺝﺭﺎﺧﻂﻴﺤﻣ

ﻞﻣﺎﻜﺗﻡﺪﻋﻪﺑﺮﺠﻨﻣﺖﺳﺍﻦﻜﻤﻣﺰﻧژﻮﻴﻣﺮﭙﺳﺍﺪﻧﻭﺭﺭﺩﻝﻼﺘﺧﺍﻪﻧﻮﮔ ﺰﻴﻧﻦﻴﺗﺎﻣﻭﺮﻛ ﻢﻛﺍﺮﺗﻭﻱﺪﻨﺑﻪﺘﺴﺑﺭﺩ ،ﻩﺪﻳﺩﺮﮔﻡﺮﭙﺳﺍﻲﻳﺎﻤﺳﻼﭘ ﺎﺸﻏ ﻦﻴﺗﺎﻣﻭﺮﻛ ﺢﻴﺤﺻ ﻢﻛﺍﺮﺗﻪﻛ ﻰﻳﺎﺠﻧﺁ ﺯﺍ .(11) ﺪﻳﺎﻤﻧ ﺩﺎﺠﻳﺍ ﻝﻼﺘﺧﺍ

ﺎﺸﻏﻲﺤﻄﺳﻱﺎﻫﻦﻴﺌﺗﻭﺮﭘﻞﻣﺎﻜﺗﺎﺑﺖﺳﺍﻦﻜﻤﻣ،ﻪﻠﺣﺮﻣﻦﻳﺍﺭﺩﻡﺮﭙﺳﺍ ﻦﻳﺍﻩﺪﻨﻨﻛ ﻪﻴﺟﻮﺗﺪﻧﺍﻮﺗﻲﻣﺮﻣﺍ ﻦﻳﺍ ،ﺪﺷﺎﺑ ﻥﺎﻣﺯﻢﻫ ﻡﺮﭙﺳﺍﻲﻳﺎﻤﺳﻼﭘ ﻯﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﺢﻴﺤﺻﻯﺍﻮﺘﺤﻣﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﻪﻛﺪﺷﺎﺑﺐﻠﻄﻣ ﺭﺩﺩﻮﺧﻰﻌﻴﺒﻃﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﺱﺎﺳﺍﺮﺑﻪﻛﻰﻳﺎﻤﺳﻼﭘ ﻯﺎﺸﻏﻰﺤﻄﺳ ﻦﻴﻣﺎﺗﻭﺮﭘﻯﺍﻮﺘﺤﻣﻯﺍﺭﺍﺩﻻﺎﻤﺘﺣﺍﺪﻧﺍﻩﺪﻳﺩﺮﮔﻯﺯﺎﺳﺍﺪﺟZetaﺵﻭﺭ

ﻪﺑﺮﺠﻨﻣﻰﻌﻴﺒﻃﻦﻴﻣﺎﺗﻭﺮﭘﻯﺍﻮﺘﺤﻣﻪﻛﻰﻳﺎﺠﻧﺁﺯﺍ .ﺪﻨﺷﺎﺑﻰﻣﺰﻴﻧﻰﻌﻴﺒﻃ

ﺩﺩﺮﮔﻰﻣDNAﺐﻴﺳﺁ ﺯﺍﻯﺮﻴﮔﻮﻠﺟﻭ ﻦﻴﺗﺎﻣﻭﺮﻛﺢﻴﺤﺻﻯﺪﻨﺑﻪﺘﺴﺑ

ﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﻪﻛﺪﺷﺎﺑﺐﻠﻄﻣﻦﻳﺍﻩﺪﻨﻨﻛﻥﺎﻴﺑﺪﻧﺍﻮﺗﻰﻣﻪﺘﻜﻧﻦﻳﺍ

DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺯﺍﻯﺮﺗﻦﻴﻳﺎﭘﺪﺻﺭﺩﻯﺍﺭﺍﺩZetaﺵﻭﺭﻪﺑﻩﺪﺷ .ﺪﻨﺷﺎﺑﻰﻣﺰﻴﻧ

ﻯﺯﺎﺳﺍﺪﺟﻥﺁﺭﺩﻪﻛﺶﻧﺍﺭﺎﻜﻤﻫﻭﺱﺭﻮﺴﻨﻳﺁﻪﻌﻟﺎﻄﻣﺭﺩﻦﻴﻨﭼﻢﻫ ﻩﺎﮕﺘﺳﺩﻂﺳﻮﺗﺎﺸﻏﺢﻄﺳ ﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﺱﺎﺳﺍﺮﺑﻰﻌﻴﺒﻃ ﻯﺎﻫﻡﺮﭙﺳﺍ ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻥﺍﺰﻴﻣﻪﻛﺪﺷﻩﺩﺍﺩﻥﺎﺸﻧ،ﺖﻓﺮﮔﺕﺭﻮﺻﺯﺭﻮﻓﻭﺮﺘﻜﻟﺍ

ﻩﺪﺷ ﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ TUNEL ﺖﺴﺗﺯﺍ ﻩﺩﺎﻔﺘﺳﺍﺎﺑ DNA

ﺩﻮﺧﻪﺘﻓﺎﻳﻦﻳﺍﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛ ،ﺵﻭﺭﻦﻳﺍﻪﺑ ﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﻥﺎﻴﻣﻯﻮﻗﻁﺎﺒﺗﺭﺍﻚﻳﺩﻮﺟﻭﺮﺑﻯﺮﮕﻳﺩﻞﻴﻟﺩﺪﻧﺍﻮﺗﻰﻣ

.(7) ﺪﺷﺎﺑﻡﺮﭙﺳﺍﻪﺘﺴﻫﺭﺎﺘﺧﺎﺳﻭﺎﺸﻏﺢﻄﺳﺮﺑﺩﻮﺟﻮﻣ

،ﻰﻌﻴﺒﻃﺮﻴﻏ ﻯﺎﻫﻡﺮﭙﺳﺍ ﺪﺻﺭﺩ ﻦﻴﺑ ﻪﻄﺑﺍﺭ ﻰﺳﺭﺮﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳﺍ ﺭﺩ

ﻭ ﺖﻓﺮﮔ ﺕﺭﻮﺻ ﻝﺮﺘﻨﻛﻩﻭﺮﮔﺭﺩ DNA ﺐﻴﺳﺁ ﻭﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ

ﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻦﻴﺑﻯﺭﺍﺩﻰﻨﻌﻣﻭﻢﻴﻘﺘﺴﻣﻁﺎﺒﺗﺭﺍﻪﻛﺩﺍﺩﻥﺎﺸﻧﺎﻫﻰﺳﺭﺮﺑ ﻁﺎﺒﺗﺭﺍﻦﻴﻨﭽﻤﻫ .(p=0/001) ﺩﺭﺍﺩﺩﻮﺟﻭﻦﻴﻣﺎﺗﻭﺮﭘﺩﻮﺒﻤﻛﻭﻰﻌﻴﺒﻃﺮﻴﻏ

ﺭﺍﺩﻰﻨﻌﻣ ﻭ ﻢﻴﻘﺘﺴﻣ ﺰﻴﻧ DNA ﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﻭ ﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ ﻦﻴﺑ

ﻩﺭﺎﺷﺍﻪﺘﻜﻧﻦﻳﺍﻪﺑﻥﺍﻮﺗﻰﻣﺐﻠﻄﻣﻦﻳﺍﻪﻴﺟﻮﺗﺭﺩ .(p=0/001) ﺪﺷﺎﺑﻰﻣ

ﻱﺪﻨﺑﻪﺘﺴﺑﺭﺩﺺﻘﻧ،DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﺩﺎﺠﻳﺍﻞﻠﻋﺯﺍﻲﻜﻳﻪﻛﺩﻮﻤﻧ

ﺰﻧژﻮﻴﻣﺮﭙﺳﺍ ﺯﺍ ﻪﻠﺣﺮﻣ ﻦﻳﺍ ﺭﺩ ﻦﻴﻣﺎﺗﻭﺮﭘ ﺩﻮﺒﻤﻛ ﻭ ﺪﺷﺎﺑﻲﻣ ﻦﻴﺗﺎﻣﻭﺮﻛ

DNAﺐﻴﺳﺁﺩﺎﺠﻳﺍﻭﻦﻴﺗﺎﻣﻭﺮﻛﺢﻴﺤﺻﻢﻛﺍﺮﺗﻡﺪﻋﻪﺑﺮﺠﻨﻣﺪﻧﺍﻮﺗﻲﻣ

ﺶﻘﻧ ﺮﮕﻧﺎﺸﻧﻪﻄﺑﺍﺭ ﻦﻳﺍﺩﻮﺟﻭ .(27) ﺩﺩﺮﮔﻲﻠﺳﺎﻨﺗ ﻩﺎﮕﺘﺳﺩﺮﻴﺴﻣﺭﺩ

ﻪﺘﺴﻫDNAﻪﺑ ﺐﻴﺳﺁﻥﺪﻴﺳﺭﺯﺍﻯﺮﻴﮔﻮﻠﺟ ﺖﻬﺟﻦﻴﻣﺎﺗﻭﺮﭘﻰﺘﻇﺎﻔﺣ

.(28-30) ﺪﺷﺎﺑﻰﻣﻡﺮﭙﺳﺍ

ﺎﺑﺵﻭﺭﻭﺩﻦﻳﺍﺯﺍﻩﺪﻣﺁﺖﺳﺩﻪﺑﻯﺎﻫﻡﺮﭙﺳﺍﻰﺳﺭﺮﺑﺞﻳﺎﺘﻧﻪﺴﻳﺎﻘﻣ

ﻯﺯﺎﺳﺍﺪﺟﺭﺩﺵﻭﺭﻭﺩﺮﻫﻪﻛﺪﻫﺩﻰﻣﻥﺎﺸﻧﺮﮕﻳﺪﻜﻳﺎﺑﻭﻝﺮﺘﻨﻛﻩﻭﺮﮔ ﻩﻭﺮﮔﻪﺑﺖﺒﺴﻧﻢﻟﺎﺳDNAﻭﻰﻌﻴﺒﻃﻦﻴﻣﺎﺗﻭﺮﭘﻯﺍﻮﺘﺤﻣﺎﺑ،ﻎﻟﺎﺑﻯﺎﻫﻡﺮﭙﺳﺍ

ﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍﺪﺻﺭﺩﻰﺳﺭﺮﺑﻪﻛﻰﻟﺎﺣﺭﺩ .ﺪﺷﺎﺑﻰﻣﺮﺛﻮﻣﺭﺎﻴﺴﺑﻝﺮﺘﻨﻛ

ﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﻪﺑﺖﺒﺴﻧDGCﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟ DNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓﻭﺪﻫﺩﻰﻣﻥﺎﺸﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛZetaﺵﻭﺭ TUNELﻚﻴﻨﻜﺗﻪﺳﺮﻫﺭﺩ Zetaﺵﻭﺭﻪﺑﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﺭﺩ

ﻩﺪﺷﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍﻪﺑﺖﺒﺴﻧﺍﺭﻯﺭﺍﺩﻰﻨﻌﻣﺶﻫﺎﻛAOﻭSCDﻭ

ﻥﺍﻮﺗﻰﻣﺐﻠﻄﻣﻦﻳﺍﻪﻴﺟﻮﺗﺭﺩ .(p<0/001) ﺪﻨﻫﺩﻰﻣﻥﺎﺸﻧDGCﺵﻭﺭﻪﺑ

ﻯﺍﺭﺍﺩﻻﺎﻤﺘﺣﺍﺮﺘﺸﻴﺑﻰﻟﺎﮕﭼﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﻪﻛﺩﺮﻛﻩﺭﺎﺷﺍﻪﺘﻜﻧﻦﻳﺍﻪﺑ ﺭﺎﺑﻯﺍﺭﺍﺩﻯﺎﻫﻡﺮﭙﺳﺍﻪﺑﺖﺒﺴﻧﻰﻌﻴﺒﻃﺮﻴﻏﻯﺎﻫﻡﺮﭙﺳﺍﺯﺍﻯﺮﺗﻦﻴﻳﺎﭘﺪﺻﺭﺩ ﺪﺷﻩﺭﺎﺷﺍﺰﻴﻧﻦﻳﺍﺯﺍﺶﻴﭘﻪﭽﻧﺎﻨﭼﻪﻛﻰﻟﺎﺣﺭﺩ،ﺪﻨﺷﺎﺑﻰﻣﻰﻌﻴﺒﻃﻰﻜﻳﺮﺘﻜﻟﺍ ﻰﻜﻳﺮﺘﻜﻟﺍﺭﺎﺑﻩﺪﻨﻨﻛﺩﺎﺠﻳﺍﻯﺎﻫﻦﻴﺌﺗﻭﺮﭘﻮﻜﻴﻠﮔﻮﻟﺎﻴﺳﻦﻴﺑﻯﻮﻗﻁﺎﺒﺗﺭﺍﺩﻮﺟﻭ ﻥﺍﺰﻴﻣﻩﺪﻨﻫﺩﻥﺎﺸﻧﺪﻧﺍﻮﺗﻰﻣ،ﻡﺮﭙﺳﺍﻪﺘﺴﻫﻦﻴﺗﺎﻣﻭﺮﻛﺖﻣﻼﺳﻭﺎﺸﻏﺢﻄﺳ ﺵﻭﺭ ﻪﺑﻩﺪﺷ ﻯﺯﺎﺳﺍﺪﺟﻯﺎﻫﻡﺮﭙﺳﺍ ﺭﺩDNAﻥﻮﻴﺳﺎﺘﻨﻤﮔﺍﺮﻓ ﺮﺗﻦﻴﻳﺎﭘ

.ﺪﺷﺎﺑDGCﺵﻭﺭﻪﺑﺖﺒﺴﻧZeta

ﻯﺮﻴﮔ

ﻪﺠﻴﺘﻧ

ﻯﺯﺎﺳﺍﺪﺟﻪﺑﺭﺩﺎﻗﻯﺭﺍﺩﻰﻨﻌﻣﺭﻮﻃﻪﺑDGCﻭ Zetaﺵﻭﺭﻭﺩﺮﻫ

ﺎﺑ .ﺪﺷﺎﺑﻰﻣ ﻰﻌﻴﺒﻃ ﻦﻴﺗﺎﻣﻭﺮﻛ ﺭﺎﺘﺧﺎﺳ ﻭ ﻯژﻮﻟﻮﻓﺭﻮﻣ ﺎﺑ ﻯﺎﻫﻡﺮﭙﺳﺍ

ﻦﻳﺍ ﺯﺍ ﺶﻴﭘ ﻪﻛ ﻲﺗﺎﻌﻟﺎﻄﻣ ﻭ ﺶﻫﻭﮋﭘ ﻦﻳﺍ ﺯﺍ ﻞﺻﺎﺣﺞﻳﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺪﻨﻤﺷﺯﺭﺍ ،ﺖﺳﺍ ﻪﺘﻓﺮﮔ ﻡﺎﺠﻧﺍ DGCﻭ Zeta ﺵﻭﺭ ﻭﺩ ﻥﻮﻣﺍﺮﻴﭘ Centrifugationﺵﻭﺭﻭﺩﺮﻫﺯﺍﻰﺒﻴﻛﺮﺗﺕﺭﻮﺻﻪﺑﻪﻛﺩﻮﺑﺪﻫﺍﻮﺧ ﻰﻌﻴﺒﻃ ﻯﺎﻫﻡﺮﭙﺳﺍﻯﺯﺎﺳﺍﺪﺟﺖﻬﺟ ،Zeta ﻭ Density Gradient .ﺩﺩﺮﮔﻩﺩﺎﻔﺘﺳﺍ

ﺮﻜﺸﺗ

ﻭ

ﺮﻳﺪﻘﺗ

ﻯﺭﻭﺭﺎﺑﺎﻧﻭ ﻯﺭﻭﺭﺎﺑﺰﻛﺮﻣ ﻭﻥﺎﻳﻭﺭﻩﺪﻜﺸﻫﻭﮋﭘ ﻯﺭﺎﻜﻤﻫﺎﺑﻖﻴﻘﺤﺗﻦﻳﺍ ﻕﻮﻓﺰﻛﺍﺮﻣﻞﻨﺳﺮﭘﻭﻦﻴﻟﻮﺌﺴﻣﻪﻴﻠﻛﺯﺍﺍﺬﻟ .ﺖﺳﺍﻪﺘﻓﺮﮔﻡﺎﺠﻧﺍﻥﺎﻬﻔﺻﺍ

ﻩﺪﻜﺸﻫﻭﮋﭘﻂﺳﻮﺗﻖﻴﻘﺤﺗﻦﻳﺍﻪﺟﺩﻮﺑ .ﻢﻳﺭﺍﺩﺍﺭﻰﻧﺍﺩﺭﺪﻗﻭﺮﻜﺸﺗﻝﺎﻤﻛ .ﺪﻳﺩﺮﮔﺖﺧﺍﺩﺮﭘﻥﺎﻳﻭﺭ

References

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