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An oxygenase-independent cholesterol catabolic pathway operates under oxic conditions.

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Figure S2. Seven C 19 intermediates, including two acidic metabolites were observed. Four of these intermediates  (1-testosterone, 1-hydroxyandrostan-3,17-dione,  17-hydroxy-1-oxo-2,3-seco-androstan-3-oic acid, and  1,17-dioxo-2,3-seco-androstan-3-oic acid
Figure 4CIII). Their elemental compositions were respectively calculated as C 19 H 28 16 O 4 Na, C 19 H 28 16 O 3 18 ONa, and C 19 H 28 16 O 2 18
Figure 4. The structure elucidation and the investigation of the ring cleavage mechanism of compound 1 (1,17-dioxo-2,3- seco  -androstan-3-oic acid, DSAO)

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