Cell-free
Cell-to-cell transmission can overcome multiple donor and target cell barriers imposed on cell-free HIV.
... for cell-free HIV were not fast enough to prevent a cell-free mode of spreading under our experimental conditions (Figure 1B and ...the cell- free extracellular virus vulnerable ...
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Elevated levels of cell-free circulating DNA in patients with acute dengue virus infection.
... of cell-free DNA in circulation is short (4–30 min) [38,39] due to the effects of serum nucleases, rapid removal from circulation by the liver and kidneys, and redistribution through organs and tissues ...
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Oral and vaginal epithelial cell lines bind and transfer cell-free infectious HIV-1 to permissive cells but are not productively infected.
... epithelial cell genome and whether productive infection ...vivo cell models, whereby adult epithelium coats HIV-1 particles with human b-defensins 2 and 3, rendering the cell-free virus less ...
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In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network
... For the network geometry with a depth of 100 μm, the results in- dicate that the CFL thickness formed at the confluence apex (CFLM) tend to increase along the network. For instance, the CFLM at CF1 is always the highest ...
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Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction
... cellular immunity in patients with PCM and in epidemiological studies is the DTH reaction with paracoccidioidin (4, 6). he paracoccidioidin that has been classically employed is the Fava-Netto polysaccharide-rich antigen ...
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In vitro Blood Flow and Cell-Free Layer in Hyperbolic Microchannels: Visualizations and Measurements
... Abstract Red blood cells (RBCs) in microchannels has tendency to undergo axial migration due to the parabolic velocity profile, which results in a high shear stress around wall that forces the RBC to move towards the ...
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Particulate Blood Analogues Reproducing the Erythrocytes Cell Free Layer in a Microfluidic Device Containing a Hyperbolic Contraction
... a cell free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen ...
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Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes.
... (cell free) expression has emerged in recent times as a promising alternative option in order to synthesize large quantities of membrane proteins, and the focus of this report was to evaluate the potential ...
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In vitro blood flow and cell-free layer in hyperbolic microchannels: Visualizations and measurements
... A well-known physiological phenomenon happening in microcirculation, named as the Fåhræus-Lindqvist effect, is the tendency of the RBCs to migrate to the centre of the microchannel and consequently leading to the ...
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Atividade antimicrobiana e antibiofilme do sobrenadante de cepas de lactobacillus cell-free sobre isolados de escherichia coli farmacoresistentes
... O desenvolvimento da biomassa bacteriana é a etapa conclusiva na formação dos biofilmes que pode desencadear resistência à ação de antibióticos, portanto, uma interrupção nessa cascata de formação é um processo complexo ...
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AVALIAÇÃO DA ATIVIDADE DE CELL-FREE DNA (cfDNA) ISOLADO DE LINHAGEM TUMORAL PROSTÁTICA EM CÉLULAS NORMAIS RECIPIENTES
... o cell-free DNA circulante (cfDNA) das linhagens celulares da próstata (PC-3 e RWPE-1), bem como avaliar a proliferação celular durante a liberação do mesmo e, analisar a linhagem normal da próstata ...
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Properties of catechol 1,2-dioxygenase in the cell free extract and immobilized extract of Mycobacterium fortuitum
... The immobilization can increase molecular stability of the immobilized enzyme, as a result of reduced thermal inactivation (Constantini et al., 2009; Mateo et al., 2000). Gottschalk and Jaenicke (1991) showed that ...
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Non-thermal radio frequency and static magnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation.
... The growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. ...
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Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach
... Experimental blood flow studies at the microscale level have been extensively active from the beginning of the twenty-first century (Popel & Johnson 2005; Lima et al. 2012). The great interest in this field was in ...
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Mutation detection in tumor-derived cell free DNA anticipates progression in a patient with metastatic colorectal cancer
... Liquid biopsies can be use to detect tumor biomarkers, such as circulating tumor cells, tumor microvesicles and circulating tumor DNA (ctDNA) in plasma and other body fluids. One of the most validated liquid biopsies ...
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Formation of hydroxyl radical from San Joaquin Valley particles extracted in a cell-free surrogate lung fluid
... Abstract. Previous studies have suggested that the ad- verse health effects from ambient particulate matter (PM) are linked to the formation of reactive oxygen species (ROS) by PM in cardiopulmonary tissues. While ...
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Particulate Blood Analogues Reproducing the Erythrocytes Cell-Free Layer in a Microfluidic Device Containing a Hyperbolic Contraction
... . Maruyama et al. [39,40] developed microcapsule suspensions in a Newtonian solvent to evaluate the absolute hemolytic properties of centrifugal blood pumps. Later on Nguyen et al. [41] elaborated similar blood analogues ...
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Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
... Eukaryotic cells have nuclear DNA (nDNA) and addi- tional cytoplasmic mitochondrial DNA (mtDNA). It has been demonstrated that cell-free nucleic acids, i.e., cell-free (ccf) nuclear DNA ...
5
Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods.
... Antioxidant activity of lactic acid bacteria is associated with multiple health-protective ef- fects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant ef- fects of these bacteria in ...
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Cell-free layer measurements ofin vitroblood flow in a microfluidic network: an automatic and manual approach
... Experimental blood flow studies at the microscale level have been extensively active from the beginning of the twenty-first century (Popel & Johnson 2005; Lima et al. 2012). The great interest in this field was in ...
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