coat protein
Molecular identifi cation based on coat protein sequences of the Barley yellow dwarf
... ABSTRACT: Yellow dwarf disease, one of the most important diseases of cereal crops world- wide, is caused by virus species belonging to the Luteoviridae family. Forty-two virus isolates obtained from oat (Avena sativa ...
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Coat protein and RNAs of Cole latent virus are not present within chloroplasts of Chenopodium quinoa-infected cells
... In order to better investigate the association of CoLV with the chloroplasts of infected cells, these organelles were isolated on Percoll gradients and the proteins and RNAs extracted and analyzed. When proteins were ...
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Hibiscus chlorotic ringspot virus coat protein is essential for cell-to-cell and long-distance movement but not for viral RNA replication.
... Hibiscus chlorotic ringspot virus (HCRSV) is a member of the genus Carmovirus in the family Tombusviridae. In order to study its coat protein (CP) functions on virus replication and movement in kenaf ...
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An isoform of eukaryotic initiation factor 4E from Chrysanthemum morifolium interacts with Chrysanthemum virus B coat protein.
... viral coat proteins are involved in the cell-to-cell movement or subcellular localization of viruses ...several coat protein molecules to its 3 9 end, apparently mimicking the function of ...
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Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: Production
... expressed protein, this antibody reacted poorly with native MP and was not suitable for immunoassays (Calegario et ...p29 protein, a structural putative coat protein of the virion (ORF 2, RNA ...
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SEQUENCES OF THE COAT PROTEIN GENE FROM BRAZILIAN ISOLATES OF Papaya ringspot virus
... Bateson et al. (1994) compared the nucleotide sequence of 13 PRSV isolates from different parts of the world, although mostly from Australia, and showed that their cp genes did not diverge more than 12%. Tennant (1996) ...
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Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
... the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern ...complete coat protein gene was amplified by ...complete coat ...
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DETECTION AND COAT PROTEIN GENE CHARACTERIZATION OF AN ISOLATE OF Grapevine virus B FROM CORKY BARK-AFFECTED GRAPEVINES IN SOUTHERN BRAZIL
... Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated ...
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Watermelon transformation with Zucchini yellow mosaic virus coat protein gene and comparison with parental cultivar
... virus coat protein (ZYMV-CP) and neomycin phosphotransferase II (NPT II) genes to the watermelon ‘Crimson Sweet’ (CS) genome, and to compare the transgenic progenies T1 and T2 with the nontransformed ...
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A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification
... full-length coat protein (cp) gene from the Garlic Mite-borne Filamentous Virus (GarMbFV) to the 3 ′-end of the Polyhedrin (polh) gene of the baculovirus Autographa californica multiple nucleopolyhedrovirus ...
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Expression of Grapevine leafroll-associated virus 3 Coat Protein Gene in Escherichia coli and Production of Polyclonal Antibodies
... the coat protein in Escherichia ...The coat protein (CP) gene of GLRaV-3, isolate Pet- 4, from grapevine ...Total protein extracts were obtained by re-suspension in lysis ...
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Differentiation of Citrus tristeza virus (CTV) isolates by cleavase fragment length polymorphism (CFLP) analysis of the major coat protein gene
... The similarity of cloned standards obtained by CFLP analysis is in close relationship with simi- larities computed at the nucleotide level. This re- sulted in very similar dendrograms obtained with both methods. ...
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ANALYSIS OF THE NUCLEOTIDE SEQUENCE OF THE COAT PROTEIN AND 3’- UNTRANSLATED REGION OF TWO BRAZILIAN Potato virus Y ISOLATES
... Two Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA ...
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Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli: Production and Use in Immunodiagnosis
... ASGV coat protein gene was digested with ...maltose-binding protein (MBP), according to manufacturer instructions using T4 DNA ligase, for 2 h at 15 o C ...
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The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast
... of protein-protein interac- tions in the two-hybrid system depends on which protein is fused to which part of the GAL4 transcription ...viral coat protein was ten times higher when the ...
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Development of Virus Resistant Transgenic Papayas Expressing the Coat Protein Gene from a Brazilian Isolate of Papaya ringspot virus
... the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica ...
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SEQUENCE ANALYSIS OF THE CAPSID PROTEIN GENE OF AN ISOLATE OF Apple stem grooving virus, AND ITS SURVEY IN SOUTHERN BRAZIL
... The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted M r of approximately 27 ...the coat protein gene showed ...
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Expressão, purificação e caracterização da proteína capsidial de Cowpea mild mottle virus e suas aplicações na detecção viral
... putative protein products are similar to those of well- characterized species of the genus Carlavirus (King et ...a coat protein and the product of ORF 6 possesses nucleic acid-binding activity ...
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Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus
... the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya ...
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Stability of Citrus tristeza virus protective isolate ‘Pêra IAC’ according to SSCP analysis of old and new lines of three sweet orange varieties
... the coat protein gene, CTV isolates present in plants of the sweet orange cultivars Pêra, Hamlin and Valencia propagated from four budwood sources: 1) old lines, 2) nucellar lines, 3) shoot-tip-grafted ...
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