Human embryonic stem cell
Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
... for cell adhesion ...culturing stem cells from both animals (12,19) and humans (20-22), to our knowledge, this is the first description of the use of microcarriers, without coating with mouse fibroblasts or ...
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Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces
... of human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) cells in osteogenic media (OIM) and in non-osteogenic media (SM) Figure 5 highlights the cell growth in osteogenic ...
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Progress in human embryonic stem cell research in the United States between 2001 and 2010.
... on human embryonic stem cell (hESCs) out of concern for the “vast ethical mine fields” associated with the creation of embryos for re- search ...of stem cell and genetic ...
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Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes.
... hESCs (CHA-hES15 cell line) were maintained as previously described [51]. hESCs were differ- entiated into hepatocytes as previously described [52] with some modifications. Briefly, hESCs were cultured for 3 days ...
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Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
... G3 Human Gene Expression 8660K Microarray (Agilent Technologies) and covered with a Gasket 8-plex slide (Agilent Technologies), the slides were hybridized for 17 hr at 65 ...
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Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Improve Recovery after Cervical Spinal Cord Injury
... interrupt axonal tract conduction between the cranial and cau- dal central nervous system. Notable differences in cervical relative to thoracic cord include enlarged neuronal pools, proximity of descending axons to their ...
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Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes.
... this stage, a significant number of fibroblast-like cells were able to attach to plastic and survive in MP media in the absence of feeders or adhesion promoting substrates. After two further passages, we obtained a ...
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Novel Human Embryonic Stem Cell Regulators Identified by Conserved and Distinct CpG Island Methylation State
... reprogrammed human dermal fibroblasts by transfection with episomal plas- mids expressing OCT4, KLF4, SOX2, L-MYC, LIN28 and a short hairpin RNA directed against p53 [32], and added similar plasmids expressing ...
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Albumin-associated lipids regulate human embryonic stem cell self-renewal.
... Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass of human ...any cell type of the adult human body, ...of human ailments, ...
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Nucleosome Organization in Human Embryonic Stem Cells.
... The fundamental repeating unit of eukaryotic chromatin is the nucleosome. Besides being involved in packaging DNA, nucleosome organization plays an important role in transcrip- tional regulation and cellular identity. ...
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Stem cells labeled with superparamagnetic iron oxide nanoparticles in a preclinical model of cerebral ischemia: a systematic review with meta-analysis
... (mouse) embryonic stem cell; FFE, fast field echo; fMSC, fetal mesenchymal stem cell; FOV, field of vision; FSE, fast spin echo; GE, gradient echo ; GFAP, glial fibrillary acidic ...
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Homozygous Inactivating Mutation in NANOS3 in Two Sisters with Primary Ovarian Insufficiency
... of human embryonic stem cell-derived germ cells have shown coexpression of NANOS3 in nuclei with important germ cell factors such as BLIMP1, VASA, and STELLA in the cytoplasm ...germ ...
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Innate immunity in human embryonic stem cells: comparison with adult human endothelial cells.
... of human disease with human embryonic stem cell (hESC)-derived cells is now close to reality, but little is known of their responses to physiological and pathological ...primary ...
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Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells
... hES cell lines of various genomic sources, Royan H5 and 6 (RH5, RH6) and induced their differentiation into erythrocytes ...early human erythropoiesis ...
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Activation of regulatory T cells during inflammatory response is not an exclusive property of stem cells.
... fluid stem cells (AFS) and sacrificed for specimen collection 24 h after LPS ...T cell subpopulations (flow-cytometry) and pulmonary neutrophil influx ...define stem cell specific effects, ...
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Gene expression profile of adult human olfactory bulb and embryonic neural stem cell suggests distinct signaling pathways and epigenetic control.
... neuroectodermal cell fate ...neural stem cell markers, such as Nestin, Prominin1, SOX1, and SOX2 ...in human embryonic NSCs is enriched in molecules known or predicted to be involved in ...
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Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells.
... blood-derived stem cells [8]. Due to the easy expansion, human embryonic stem cells (hESCs) represent an alternative source of VSMCs particularly for old patients having stem cells with ...
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Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media.
... for stem cell-based therapies and fertility ...Three cell lines (CHO, HEK, and C18-4), mouse embryonic stem (mES) cells, and primary cells, such as mouse embryonic fibroblast ...
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Variations of X chromosome inactivation occur in early passages of female human embryonic stem cells.
... Human embryonic stem cells (hESCs) are an invaluable tool for regenerative medicine and a model for early human embryogen- esis ...same cell line ...
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Expression and secretion of human recombinant LIF by genetically modified mammalian cells
... Gene expression in human neural stem cells: effects of leukemia inhibitory factor // J.. Embryonic stem cells capable of differen- tiating into desired cell lines / J.[r] ...
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