Internal transcribed spacer
Comparison of the sequences of the internal transcribed Spacer regions and PbGP43 genes of Paracoccidioides brasiliensis from patients and armadillos (Dasypus novemcinctus)
... Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were compara- ble with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal ...
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Genetic Analyses of the Internal Transcribed Spacer Sequences Suggest Introgression and Duplication in the Medicinal Mushroom Agaricus subrufescens.
... The internal transcribed spacer (ITS) region of the nuclear ribosomal RNA gene cluster is widely used in fungal taxonomy and phylogeographic ...
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Ribosomal internal transcribed spacer of Prototheca wickerhamii has characteristic structure useful for identification and genotyping.
... ribosomal internal transcribed spacer (ITS) were different in a species-dependent manner, suggesting that the variation might be useful for differentiation of Prototheca ...
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Comparison of the sequences of the internal transcribed Spacer regions and PbGP43 genes of Paracoccidioides brasiliensis from patients and armadillos (Dasypus novemcinctus)
... Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were compara- ble with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal ...
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Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)
... ond internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) from 16 species of the genus Culex and one of Lutzia were used to assess their genomic variability and to verify their applicability in ...
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Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi
... The internal transcribed spacer (ITS) of the rDNA re- gion of 26 isolates belonging to 8 genera of ectomy- corrhizal fungi showed extensive length polymorphism (Figure 1), PCR amplification with ...
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The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy - A critical review
... Among the molecular markers commonly used for mosquito taxonomy, the internal transcribed spacer 2 (ITS2) of the ribosomal DNA is useful for distinguishing among closely-related species. Here we ...
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Intragenomic Profiling Using Multicopy Genes: The rDNA Internal Transcribed Spacer Sequences of the Freshwater Sponge Ephydatia fluviatilis.
... of internal transcribed spacer sequences found within every rDNA repeat unit by implementing direct sequencing ...the internal transcribed spacers of the freshwater sponge Ephydatia ...
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Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes
... Based on sequence derived from cloning of ITS2 (Li & Wilkerson, unpublished data, Table I) we designed a se- ries of six ITS2 internal primers that can be used for iden- tification of the four species in the ...
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Classification of dermatophytes by a multilocus phylogenetic approach based on tef-1α, beta tubulin and its genes
... DNA, Internal transcribed spacer (ITS) , Beta tubulin gene (BT2) and the nucleotide sequence of translation elongation factor 1-α (Tef-1α) were used as tools to identify phylogenetically these four ...
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ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the
... DNA internal transcribed spacer 2 (ITS2) has been shown to be a useful genetic marker for species identification and phylogenetic reconstruction in the genus of freshwater snails Biomphalaria ...
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Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies
... Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS ...
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Omar S Carvalho+ , Paula CM Cardoso, Pollanah M Lira, Alejandra Rumi, Andrea Roche, Elizabeth Berne, Gertrud Müller, Roberta L Caldeira
... Fig. 1: silver-stained 6% polyacrylamide gel showing restriction fragment length polymorphism profiles obtained after digestion of the rDNA first internal transcribed spacer with DdeI enzyme. Lanes - ...
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Molecular phylogeny of the Myzorhynchella Section of Anopheles (Nyssorhynchus) (Diptera: Culicidae): genetic support for recently described and resurrected species
... Phylogenetic relationships among species of the Myzorhynchella Section of Anopheles ( Nyssorhynchus) were investigated using the nuclear ribosomal DNA second internal transcribed spacer (ITS2), the ...
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Teofânia HDA Vidigal, Roberta Lima Caldeira, Andrew JG Simpson, Omar S Carvalho
... Fig. 7: (AluI) silver stained polycrylamide gel (6% or 8%) showing the restriction fragment length polymorphism profiles obtained following digestion of rDNA internal transcribed spacer with HpaII ...
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Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
... first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon ...
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Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification
... 16S-23S internal transcribed spacer (PCR ribotyping) and tRNA intergenic spacer (tDNA- PCR) were evaluated for their effectiveness in identification of clinical strains of Klebsiella ...
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Caracterização molecular de isolados de nematóides entomopatogênicos, Heterorhabditis...
... and internal transcribed spacer (ITS) sequence data, the strains LPP1, LPP2 and LPP4 were identified as Heterorhabditis indica, whereas LPP7 was considered Heterorhabditis ...
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Patterns of post-glacial genetic differentiation in marginal populations of a marine microalga.
... The phylogenetic analyses (Fig. 2.) showed that Baltic strains of Alexandrium ostenfeldii form a monophyletic group. Within the Baltic clade the ITS sequence did not show high differentiation. Minor nucleotide changes ...
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DNA multigene sequencing of topotypic specimens of the fascioliasis vector Lymnaea diaphana and phylogenetic analysis of the genus Pectinidens (Gastropoda)
... Freshwater lymnaeid snails are crucial in defining transmission and epidemiology of fascioliasis. In South Amer- ica, human endemic areas are related to high altitudes in Andean regions. The species Lymnaea diaphana has, ...
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