intracellular amastigotes
A screen against Leishmania intracellular amastigotes: comparison to a promastigote screen and identification of a host cell-specific hit.
... the intracellular parasite stages, we screened the same set of 909 compounds against both ...the intracellular amastigote screen, defined as inducing 60% parasite growth inhibition at 10 m M, were also ...
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Production of Amastigotes from Metacyclic Trypomastigotes of Trypanosoma cruzi
... cruzi amastigotes are known to differentiate from trypomastigotes and metacyclic trypomastigotes, it has only been possible to generate amastigotes in vitro from the tissue-culture-derived ...into ...
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Fusão Homotípica e Heterotípica entre Vacúolos Parasitóforos de Leishmania spp
... non-viral intracellular pathogens lodge within cell vesicles known as ‘‘parasitophorous vacuoles’’ (PVs), which exhibit a variety of pathogen-dependent functional and compositional ...and intracellular ...
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In Vitro and in Vivo Activity of an Organic Tellurium Compound on Leishmania (Leishmania) chagasi
... leishmanicidal activity of RF07 against intracellular amastigotes, whereas the drug displayed 10-fold less toxicity to macrophages. Although similar leishmanicidal effect was observed with Glucan- time, ...
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Unlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal
... Although there are reports of the traditional use of different halophytic species as anti-parasitic and/or anti-helminthic agents [9] , to the best of our knowledge there is no scienti fic information regarding the ...
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Miltefosine and antimonial drug susceptibility of Leishmania Viannia species and populations in regions of high transmission in Colombia.
... for intracellular amastigotes of 245 clinical strains of the most prevalent Leishmania Viannia species in Colombia to miltefosine (HePC) and/or meglumine antimoniate (Sb V ); 163, (80%) were evaluated for ...
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1,4-Diamino-2-butanone, a putrescine analogue, promotes redox imbalance in Trypanosoma cruzi and mammalian cells
... of intracellular parasites ...of intracellular amastigotes may be related to the impairment of nutrients sus- tained by the pool of thiols, as observed here when LLC-MK2 cells were treated with DAB ...
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Efficacy of sertraline against Trypanosoma cruzi: an in vitro and in silico study
... placed every 24 h. Then, untreated and treated infected cardiac cell cultures were fixed and stained with Giemsa solution, and the mean number of infected host cells and mean number of parasites per infected cell were ...
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A Role for Extracellular Amastigotes in the Immunopathology of Chagas Disease
... some intracellular amastigotes by the time the trypomastigote-specific CTL in- duced death by ...few intracellular trypomastigotes should suffice to ensure long term survival of ...released ...
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Kharon1 null mutants of Leishmania mexicana are avirulent in mice and exhibit a cytokinesis defect within macrophages.
... Δkh1 intracellular amastigotes and confirm a cytokinesis defect Previous ultrastructural studies in one of our laboratories ...on intracellular amastigotes described a putative parasite ...
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Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
... but upon treatment with melittin, a strong inhibition of these metabolites was observed. These observations corroborates Kwon et al. [48] and Moon et al. [49], who demonstrated the capacity of melittin to inhibit NO ...
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Diterpenoids from Azorella compacta (Umbelliferae) active on Trypanosoma cruzi
... Our work shows that azorellanol (Y-2) and mulin-11,3- dien-20-oic acid (Y-5) have trypanocidal effects against all developing stages of T. cruzi. These appears to be an interesting attribute of these class of compounds, ...
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In vitro evaluation of new terpenoid derivatives against Leishmania infantum and Leishmania braziliensis
... The activity of five (1-5) abietane phenol derivatives against Leishmania infantum and Leishmania braziliensis was studied using promastigotes and axenic and intracellular amastigotes. Infectivity and ...
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Investigation into in vitro anti-leishmanial combinations of calcium channel blockers and current anti-leishmanial drugs
... the intracellular amastigote ...and intracellular amastigotes) can provide additional information about the distinct action of the drug combinations in addition to that obtained from ordinary ...
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Detection of intracellular Helicobacter pylori in
... detect intracellular inclusion by direct examination using optical microscopy of the yeast samples, obtained from the oral mucosa of new- borns, in only one of these samples (corresponding to ...
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Cell-Responsive Nanogels for Anticancer Drug Delivery
... the intracellular reductive environment, which could rapidly be internalized by CAL-72 cells, resulting in higher Dox intracellular accumulation, and a notable cell death when compared with free ...
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On the Computing Potential of Intracellular Vesicles.
... With regards to achieving vesicle synchronisation, as slime mould calcium transport is heavily linked to shuttle streaming [24]—the rhythmic bi-directional flow of cytoplasm insti- gated by muscle protein contraction and ...
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Rev. Soc. Bras. Med. Trop. vol.50 número2
... FIGURE 3 - Leishmania infantum amastigotes detected inside the nodules by immunohistochemical analysis in spleen of naturally infected dog (400x). Acknowledgments[r] ...
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J. Braz. Chem. Soc. vol.28 número12
... the amastigotes of Trypanosoma cruzi by evaluating the ultrastructural alterations, membrane permeability, mitochondrial membrane potential and cell volume, since the majority of compounds displayed promising ...
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Rev. Saúde Pública vol.45 número4
... Las biopsias de los ratones inoculados con homo- genizados infectivos (tripomastigotes de T. cruzi) fueron analizadas por microscopia de luz, observán- dose que todas las cepas tenían un comportamiento histopatológico ...
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