MALDI-TOF MS
Gram-stain plus MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) for a rapid diagnosis of urinary tract infection.
... and MALDI-TOF MS mass spectrometry (MS) on urine samples to anticipate clinically useful ...for MALDI-TOF ...to MALDI-TOF ...of MALDI-TOF MS ...
7
Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection
... Using a yellow tip a tiny sample (about 10 6 yeasts per sample) was transferred directly from a single colony and spotted onto the 48 well flex target plate (FlexiMass, Shi- madzu Biotech, UK). Aliquots of 0.5 mL of 25% ...
8
Differentiation of Bacillus pumilus and Bacillus safensis using MALDI-TOF-MS.
... by MALDI-TOF-MS using the Andromas database ...the MS analysis, suggested the presence of a series of ions at m/z 3620, 5297, 6617 and 7237, which were specific for this species, when compared ...
10
MALDI-TOF MS as a tool for the identification of Vibrio alginolyticus from Perna perna mussels (Linnaeus, 1758)
... 75%. MALDI-TOF MS and DNA sequence-based species classifications are comparable (Alatoom et ...of MALDI-TOF MS-based Aeromonas species classifications by gyrB sequence analysis; ...
7
Aplicação de MALDI-TOF MS na caracterização de microalgas da família Selenastraceae (Chlorophyta)
... The morphological variability in the Selenastraceae family has been a barrier to a good establishment of well-defined taxonomic groups. Several studies with molecular compounds have already demonstrated its importance in ...
48
Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation.
... (MALDI-TOF MS) is able to identify bacterial species via determination of highly specific pro- tein mass-spectra profiles within minutes and has revolutionized the identification of bacteria ...[6]. ...
6
HPLC-UV, MALDI-TOF-MS and ESI-MS/MS analysis of the mechlorethamine DNA crosslink at a cytosine-cytosine mismatch pair.
... used MALDI-TOF-MS for determination of the molecular weight of the crosslinked duplex, followed by enzymatic digestion to obtain a fragment containing the specific crosslinked nucleosides for ...
9
Evaluation of MALDI-TOF MS in the microbiology laboratory
... The Biotyper system was conceived and marketed exclusively by Bruker Daltonics and is capable of analyzing specimens including bacterial, mycobacterial, and fungal samples in addition to samples recovered directly from ...
7
A utilização da espectrometria de massa MALDI-TOF na identificação de microrganismos no controle de qualidade farmacêutico
... número de estudos de componentes das superfícies das células bacterianas foi analisado e também uma ampla variedade de sistemas de solventes foram utilizados para extrair o material proteico a partir da superfície de ...
41
J. Braz. Chem. Soc. vol.26 número3
... (MALDI-TOF MS) followed by statistical analysis revealed that these strains could be clustered into three different groups, consistent with their enzymatic activity ...the MALDI-TOF ...
8
Identification of altered plasma proteins by proteomic study in valvular heart diseases and the potential clinical significance.
... 2-DE is currently a widely used analytical method for proteomic studies. It allows the separation of a mixture of proteins by intrinsic charges in the first dimension and by relative molecular masses in the second ...
9
MALDI-TOF mass spectrometry detection of pathogens in vectors: the Borrelia crocidurae/Ornithodoros sonrai paradigm.
... the MALDI-TOF-MS profiling, the 14 Borrelia strains included in the database were further blindly analyzed by MALDI-TOF-MS as described ...
6
Detection of SPM-1-Producing Pseudomonas aeruginosa and Class D beta-Lactamase-Producing Acinetobacter baumannii Isolates by Use of Liquid Chromatography-Mass Spectrometry and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
... and MALDI-TOF MS assays were performed by incubating fresh bacterial inoculums from Mueller-Hinton agar plates (Oxoid, Basingstoke, United Kingdom) in a buffer-adjusted solution (20 mM Tris-HCl, pH ...
4
Identification and Antibiotic-Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends
... describe MALDI-TOF MS as a possible alternative technology for AST, [58] by directly analyzing the enzymatic reaction at the molecular level (Figure ...through MALDI-TOF ...
18
UNIVERSIDADE FEDERAL DE SÃO CARLOS CENTRO DE CIÊNCIAS EXATAS E DE TECNOLOGIA DEPARTAMENTO DE QUÍMICA PROGRAMA DE PÓS-GRADUAÇÃO EM QUÍMICA “INVESTIGAÇÃO DO POTENCIAL ANTIMICROBIANO DE Casearia sylvestris SOBRE MICRORGANISMO ENDOSSIMBIONTE DE Diabrotica spe
... por MALDI-TOF/MS, e após a obtenção de agrupamentos estatísticos alguns microrganismos foram identificadas através do seqüenciamento de rDNA região ...
195
Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
... using MALDI-TOF MS ...the MALDI-TOF MS-based identification of clinically relevant moulds in the routine laboratory ...for MALDI-TOF MS-based identification ...
9
Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
... by MALDI MSI in longitudinal and transversal orientations in mature barley and at seven time points during germination (Fig 1, S4 ...(FT-ICR) MS imaging at Bruker Daltonics in Bremen, ...and ...
25
Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry.
... hypothesis, MS peptidomic analyses were employed on a subset of the study samples used for DSC ...analysis. MALDI-TOF MS based peptidomic analyses of serum, plasma and urine has previously ...
12
J. Braz. Chem. Soc. vol.23 número12
... Por meio de técnicas cromatográficas, um novo peptídeo aniônico, denominado otacidina, foi isolado das secreções cutâneas de três anuros brasileiros Osteocephalus taurinus. O sequenciamento de novo dos aminoácidos foi ...
4
Identificação direta de microrganismos causadores de mastite por espectrometria de...
... Após está preparação as amostras foram submetidas ao protocolo de lise bacteriana para análise por MALDI-TOF MS descrito por Lartigue et al. (2009) e Barreiro et al. (2010). O pellet de bactérias ...
88